Purple noise-induced changes in the expression of SLC26A4 gene in cochlear tissue of rat
Journal of Behdasht dar Arseh (i.e., Health in the Field),
Vol. 8 No. 4 (1399),
12 July 2021
,
Page 1-11
https://doi.org/10.22037/jhf.v8i4.33987
Abstract
Background and Aims: As one of the most important physical detrimental factors, purple noise can be considered similar to sounds that a wide range of people are exposed to in their workplaces. Also, cochlear tissue samples are the best tissue samples to evaluate gene expression and pathologic studies following noise exposure. Therefore, the aim of this study was to investigate the effects of purple noise on the expression of SLC26A4 gene in cochlear tissue.
Methods & material: Totally, 10 male Wistar rats were used in this experimental study. Both N1 and N2 exposure groups were exposed to purple noise with sound pressure level of 115-120 dB and frequency range of 4-20 kHz. Histological tests were performed for pathological studies. Finally, the relative expression of SLC26A4 gene was determined by qRT-PCR technique. All experiments were conducted according to ethical standards of working with laboratory animals.
Results: The results showed that the expression of SLC26A4 gene decreased significantly compared to the normal level in both N1 and N2 groups. The results of cochlear tissue pathology showed that the group exposed to purple noise for 6 days had more mechanical damage 7 days after cessation of exposure.
Conclusion: Significant decreased expression in SLC26A4 gene and permanent damage to the Reissner and Basal lamina membranes in the cochlear tissue and the auditory nerve ganglion leads to strengthening the incidence of advancing sensorineural hearing impairment and increases the likelihood of metastasis in cochlear tissue.
- Purple noise, SLC26A4 expression, Cochlear tissue, Sensorineural hearing impairment
How to Cite
References
- Petit C. From deafness genes to hearing mechanisms: harmony and counterpoint. Trends in Molecular Medicine 2006; 12(2):57-64.
- Mohseni M, Azizi F, Bazazzzadegan N, Dehghani A, Sayfati M, Taghdiri M, Jamali P, et al. Screening of DFNB4 locus in Iranian Families with Hereditary Hearing Impairment. Iranian Journal of Endocrinology and Metabolism 2008; 10(4):395-400 (In Persian).
- Park H, Shaukat S, Liu XZ, Hahn S, Naz S, Ghosh M, et al. Origins and frequencies of SLC26A4 (PDS) mutations in east and south Asians: global implications for the epidemiology of deafness. Journal of Medical Genetics 2003; 40(4):242-8.
- Zhou X-X, Chen S, Xie L, Ji Y-Z, Wu X, Wang W-W, et al. Reduced Connexin26 in the mature cochlea increases susceptibility to noise-induced hearing loss in mice. International journal of Molecular Sciences 2016; 17(3):301-308.
- Sun D, Li X. The effect of noise exposure on the expression of NOS mRNA in the cochlea. Lin chuang er bi yan hou ke za zhi. Journal of Clinical Otorhinolaryngology 2000; 14(8):373-4.
- Pfenninger K, Maylie-Pfenninger M-F, Friedman L, Simkowitz P. Lectin labeling of sprouting neurons: III. Type-specific glycoconjugates on
growth cones of different origin. Developmental Biology 1984; 106(1):97-108.
- Zare S, Hasheminejad N, Elahi Shirvan H, Hasanvand D, Hemmatjo R, Ahmadi S. Assessing Individual and Environmental Sound Pressure Level and Sound Mapping in Iranian Safety Shoes Factory. Romanian Journal of Acoustics and Vibration 2018; 15(1):20-5 (In Persian).
- Chen G-D. Prestin gene expression in the rat cochlea following intense noise exposure. Hearing Research 2006; 222(1-2):54-61.
- Ninomiya H, Ohgami N, Oshino R, Kato M, Ohgami K, Li X, et al. Increased expression level of Hsp70 in the inner ears of mice by exposure to low frequency noise. Hearing Research. 2018; 36(3):49-54.
- Gong T-W, Fairfield DA, Fullarton L, Dolan DF, Altschuler RA, Kohrman DC, et al. Induction of heat shock proteins by hyperthermia and noise overstimulation in Hsf1−/− mice. Journal of the Association for Research in Otolaryngology 2012; 13(1):29-37.
- Patel M, Hu Z, Bard J, Jamison J, Cai Q, Hu BH. Transcriptome characterization by RNA-Seq reveals the involvement of the complement components in noise-traumatized rat cochleae. Neuroscience 2013; 24(8):1-16.
- Du F, Yin L, Shi M, Cheng H, Xu X, Liu Z, et al. Involvement of microglial cells in infrasonic noise-induced stress via upregulated expression of corticotrophin releasing hormone type 1 receptor. Neuroscience 2010; 167(3):909-19.
- Han Y, Wang X, Chen J, Sha SH. Noise‐induced cochlear F‐actin depolymerization is mediated via ROCK 2/p‐ERM signaling. Journal of Neurochemistry 2015; 133(5):617-28.
- Gratton MA, Eleftheriadou A, Garcia J, Verduzco E, Martin GK, Lonsbury–Martin BL, et al. Noise-induced changes in gene expression in the cochleae of mice differing in their susceptibility to noise damage. Hearing Research 2011; 277(1-2):211-26.
- Stansfeld SA, Matheson MP. Noise pollution: non-auditory effects on health. British Medical Bulletin 2003; 68(1):243-57.
- Yildirim I, Kilinc M, Okur E, Tolun FI, Kilic MA, Kurutas EB, et al. The effects of noise on hearing and oxidative stress in textile workers. Industrial Health 2007; 45(6):743-9.
- Alagramam KN, Stepanyan R, Jamesdaniel S, Chen DH-C, Davis RR. Noise exposure immediately activates cochlear mitogen-activated protein kinase signaling. Noise & Health 2014; 16(73):400-406.
- Martins FTA, Ramos PZ, Svidnicki MCCM, Castilho AM, Sartorato EL. Optimization of simultaneous screening of the main mutations involved in non-syndromic deafness using the TaqMan® OpenArray™ Genotyping Platform. BMC Medical Genetics 2013; 14(1):112-120.
- Duman D, Tekin M. Autosomal recessive nonsyndromic deafness genes: a review. Frontiers in Bioscience: A Journal and Virtual Library 2012; 17(2):212-218.
- Birkenhäger R, Aschendorff A, Schipper J, Laszig R. Non-syndromic hereditary hearing impairment. Laryngo-Rhino-Otologie 2007; 86(4):299-309.
- Hsu W-C, Wang J-D, Hsu C-J, Lee S-Y, Yeh T-H. Expression of connexin 26 in the lateral wall of the rat cochlea after acoustic trauma. Acta Oto-Laryngologica 2004; 124(4):459-63.
- Zhang MY, Chen C, Xie XJ, XU SL, Guo GZ, Jin W. Damage to hippocampus of rats after being exposed to infrasound. Biomedical and Environmental Sciences 2016; 29(6):435-42.
- Nejat M, Riaz alhosseini Y, Arzhangi S, Bazazzadegan N, Toutounchi M, Najmabad H, et al. Frequency of Conexin 26 gene mutations in autosomal recessive deaf non-syndrome patients in Kermanshah province. Journal of Kermanshah University of Medical Sciences (Behbood) (2002-4); 9(2):32-40 (In Persian).
- Uyguner O, Emiroglu M, Uzumcu A, Hafiz G, Ghanbari A, Baserer N, et al. Frequencies of gap‐and tight‐junction mutations in Turkish families with autosomal‐recessive non‐syndromic hearing loss. Clinical Genetics 2003; 64(1(:65-9.
- Yang W, Vethanayagam RR, Dong Y, Cai Q, Hu BH. Activation of the antigen presentation function of mononuclear phagocyte populations associated with the basilar membrane of the cochlea after acoustic overstimulation. Neuroscience 2015; 30(3):1-15.
- Yang S, Cai Q, Vethanayagam RR, Wang J, Yang W, Hu BH. Immune defense is the primary function associated with the differentially expressed genes in the cochlea following acoustic trauma. Hearing Research 2016; 33(3):283-94.
- Manikandan S, Srikumar R, Parthasarathy NJ, Devi RS. Protective effect of acorus calamus L INN on free radical scavengers and lipid peroxidation in discrete regions of brain against noise stress exposed rat. Biological and Pharmaceutical Bulletin 2005; 28(12):23-30.
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