ارزیابی کارآمدی سامانه نوآورانه هیبریدی فرایند تخمیر تاریکی و سلول الکترولیز میکروبی با هدف تولید بیوهیدروژن و بهبود عملکردی فرایند آبگیری لجنهای اولیه و مازاد بیولوژیکی فاضلاب
فصلنامه علمی پژوهشی بهداشت در عرصه,
دوره 13 شماره 3 (1404),
10 ژوئن 2026
,
صفحه 3-18
https://doi.org/10.22037/jhf.v13i3.51063
چکیده
زمینه و اهداف: تولید بیوهیدروژن از لجن فاضلاب، راهکاری دوگانه برای تأمین انرژی پایدار و مدیریت مؤثر پسماند به شمار میرود. هدف از این پژوهش، امکانسنجی به کارگیری روش ترکیبی فرایند تخمیر تاریکی و سلول الکترولیز میکروبی با هدف افزایش بازده تولید هیدروژن و ارتقای همزمان کارآمدی مرحله آبگیری لجن فاضلاب شهری است.
مواد و روشها: نمونههای لجن اولیه و لجن مازاد بیولوژیکی از تصفیهخانه جنوب تهران (واحدهای 1 تا 4) برداشت شدند و مورد پیش تصفیه حرارتی قرار گرفتند. سپس به سامانههای فرایند تخمیر تاریکی و ترکیبی فرایند تخمیر تاریکی و سلول الکترولیز میکروبی تک مرحلهای منتقل شدند. بازده تولید بیوهیدروژن و کارایی فرایند آبگیری بر اساس پارامترهای مقدار pH، اسیدهای چرب فرار، میزان تولید هیدروژن، مقاومت ویژه فیلتراسیون، زمان مکش موئینهای و ویژگیهای ساختار مواد پلیمری برون سلولی بررسی شد. این پژوهش با پایبندی کامل به اصول و ملاحظات اخلاقی در تمام مراحل انجام شد.
یافتهها: سامانه ترکیبی موجب افزایش قابل توجه بازده تولید هیدروژن در pH برابر 6، در لجن اولیه 38/13 و در لجن مازاد بیولوژیکی 19/66 میلیلیتر بر گرم جامدات فرار نسبت به فرایند تخمیر تاریکی گردید. همچنین، این سامانه سبب تخریب لجن و آزادسازی آب محبوس گردید؛ بهطوری که کارایی آبگیری پذیری لجن خروجی از آن ارتقا یافت.
نتیجهگیری: به کارگیری سامانه ترکیبی به همراه پیش تصفیه حرارتی، ضمن افزایش بازده تولید بیوهیدروژن، عملکرد فرآیند آبگیری لجن فاضلاب شهری را بهبود میبخشد و میتواند به عنوان راهکاری کارآمد برای مدیریت بهینه لجن فاضلاب و تأمین انرژی پاک مطرح شود.
- تصفیه لجن
- فرایند تخمیر تاریکی
- سلول الکترولیز میکروبی
- تولید بیوهیدروژن
- آبگیریپذیری
ارجاع به مقاله
مراجع
1-Gomonov K, Permana CT, Handoko CT. The growing demand for hydrogen: сurrent trends, sectoral analysis, and future projections. Unconventional Resources 2025; 6:100176. Doi: 10.1016/j.uncres.2025.100176.
2- Zhang Q, Jiao Y, He C, Ruan R, Hu J, Ren J, et al. Biological fermentation pilot-scale systems and evaluation for commercial viability towards sustainable biohydrogen production. Nature communications 2024; 15(1):4539. Doi: 10.1038/s41467-024-48790-4.
3- Cheng D, Ngo HH, Guo W, Chang SW, Nguyen DD, Zhang S, et al. Impact factors and novel strategies for improving biohydrogen production in microbial electrolysis cells. Bioresource Technology 2022; 346:126588. Doi: 10.1016/j.biortech.2021.126588.
4- Yang G, Wang J. Enhancing biohydrogen production from disintegrated sewage sludge by combined sodium citrate-thermal pretreatment. Journal of Cleaner Production 2021; 312:127756. Doi: 10. 1016/ j. jclepro. 2021. 127756.
5- Wei Y, Zhao H, Qi X, Yang T, Zhang J, Chen W, et al. Direct interspecies electron transfer stimulated by coupling of modified anaerobic granular sludge with microbial electrolysis cell for biogas production enhancement. Applied Energy 2023; 341:121100. Doi: 10. 1016/ j. apenergy. 2023.121100.
6- Qin J, Zhu Z, Chen Z, Wang X, Zhang Y, Chen H. Synthesis, characterization and application of dewatered municipal sludge-based creamsite and its phosphorus adsorption characteristics. Journal of Cleaner Production 2023; 391:136216. Doi: 10. 1016/ j. jclepro. 2023. 136216.
7- Li XH, Liang DW, Bai YX, Fan YT, Hou HW. Enhanced H2 production from corn stalk by integrating dark fermentation and single chamber microbial electrolysis cells with double anode arrangement. International Journal of Hydrogen Energy 2014; 39(17):8977-82.
8- Ahmad A, Rambabu K, Hasan SW, Show PL, Banat F. Biohydrogen production through dark fermentation: Recent trends and advances in transition to a circular bioeconomy. International Journal of Hydrogen Energy 2024; 52:335-57.
9- Akbay HEG, Deniz F. Enhancement of biohydrogen production efficiency in dark co-fermentation of sewage sludge and fruit processing wastes through ultrasonic, alkali, acid, and thermal pretreatment. International Journal of Hydrogen Energy 2025; 131:70-81.
10- Zhou P, Li D, Zhang C, Ping Q, Wang L, Li Y. Comparison of different sewage sludge pretreatment technologies for improving sludge solubilization and anaerobic digestion efficiency: A comprehensive review. Science of the Total Environment 2024; 921:171175. Doi: 10.1016/j.scitotenv.2024.171175.
11- Mitraka GC, Kontogiannopoulos KN, Batsioula M, Banias GF, Zouboulis AI, Kougias PG. A comprehensive review on pretreatment methods for enhanced biogas production from sewage sludge. Energies 2022; 15(18):6536.
12- Usman M, Kavitha S, Kannah Y, Yogalakshmi K, Sivashanmugam P, Bhatnagar A, Kumar G. A critical review on limitations and enhancement strategies associated with biohydrogen production. International Journal of Hydrogen Energy 2021; 46(31):16565-90.
13- Zhang Y, Xu S, Cui M, Wong JW. Effects of different thermal pretreatments on the biodegradability and bioaccessibility of sewage sludge. Waste Management 2019; 94:68-76.
14- Marone A, Ayala Campos OR, Trably E, Carmona Martínez AA, Moscoviz R, Latrille E, et al. Coupling dark fermentation and microbial electrolysis to enhance bio-hydrogen production from agro-industrial wastewaters and by-products in a bio-refinery framework. International Journal of Hydrogen Energy 2017; 42(3):1609-21.
15- Posadas-Hernández M, García-Rojas JL, Khamkure S, García-Sánchez L, Gutierrez-Macías T, Morales-Morales C, et al. Enhanced biohydrogen production in a membraneless single-chamber microbial electrolysis cell during high-strength wastewater treatment: effect of electrode materials and configurations. International Journal of Hydrogen Energy 2023; 48(2):495-513.
16- Cao B, Zhang T, Zhang W, Wang D. Enhanced technology based for sewage sludge deep dewatering: A critical review. Water Research 2021; 189:116650. Doi: 10. 1016/ j. watres. 2020.116650.
17- Masihi H, Gholikandi GB. Employing Electrochemical-Fenton process for conditioning and dewatering of anaerobically digested sludge: A novel approach. Water Research 2018; 144:373-82. Doi: 10. 1016/ j. watres. 2018.07.054.
18- Zeng Q, Huang H, Tan Y, Chen G, Hao T. Emerging electrochemistry-based process for sludge treatment and resources recovery: A review. Water Research 2022; 209:117939. Doi: 10.1016/j.watres.2021.117939.
20- Yang G, Wang J. Enhancing biohydrogen production from waste activated sludge disintegrated by sodium citrate. Fuel 2019; 258:116177. Doi: 10. 1016/ j. fuel. 2019. 116177.
20- Oosterhuis M, Ringoot D, Hendriks A, Roeleveld P. Thermal hydrolysis of waste activated sludge at Hengelo wastewater treatment plant, the Netherlands. Water Science and Technology 2014; 70(1):1-7.
21-Syaichurrozi I, Hidayatullah MA, Nurullah A, Suhendi E, Kustiningsih I, Susanti DY, et al. Enhanced biohydrogen production from palm oil mill effluent using single-stage process of dark fermentation and microbial electrolysis cell at various initial pHs. Renewable Energy 2025; 249:123161. Doi: 10. 1016/ j. renene. 2025.123161.
22- Baird RB, Eaton AD, Rice EW, eds. Standard methods for the examination of water and wastewater. 23rd ed. Washington DC: American Public Health Association 2017.
23- Sanchis-Perucho P, Torres KMM, Ferrer J, Robles A. Evaluating the potential of off-line methodologies to determine sludge filterability from different municipal wastewater treatment systems. Chemical Engineering Journal 2023; 468:143537. Doi: 10.1016/j.cej.2023.143537.
24- Sadat Hosseini SA, Badalians Gholikandi G. Performance improvement of thickening and dewatering processes of biological excess sludge and anaerobic digested sludge of municipal wastewater using acid-thermally modified Nano-Montmorillonite. Journal of Health in the Field 2025; 12(4):51-63 (In Persian).
25- Oshiki M, Yamaguchi G, Takahashi K, Okabe S, Kawano S, Nakagawa J, et al. Thermophilic dark fermentation for hydrogen and volatile fatty acids production from breadcrumbs. Chemical Engineering Journal 2024; 501:157633. Doi: 10. 1016/ j. cej. 2024. 157633.
26- Alvarez AJ, Fuentes KL, Arias CA, Chaparro TR. Production of hydrogen from beverage wastewater by dark fermentation in an internal circulation reactor: Effect on pH and hydraulic retention time. Energy Conversion and Management: X 2022; 15:100232. Doi: 10.1016/j.ecmx.2022.100232.
27- Sun X, Wang Z, Wu W, Zhou S, Li J, Yan B, et al. Dark fermentation of biomass for enhanced hydrogen production: A review of pretreatment strategies, microbial enhancement, and process regulation. International Journal of Hydrogen Energy 2025; 175:151546. Doi: 10.1016/j.ijhydene.2025.151546.
28- Tang T, Chen Y, Liu M, Du Y, Tan Y. Effect of pH on the performance of hydrogen production by dark fermentation coupled denitrification. Environmental Research 2022; 208:112663. Doi: 10. 1016/ j. envres. 2021. 112663.
29- Li X, Guo L, Liu Y, Wang Y, She Z, Gao M, Zhao Y. Effect of salinity and pH on dark fermentation with thermophilic bacteria pretreated swine wastewater. Journal of Environmental Management 2020; 271:111023.
30- Ziara RM, Miller DN, Subbiah J, Dvorak BI. Lactate wastewater dark fermentation: the effect of temperature and initial pH on biohydrogen production and microbial community. International Journal of Hydrogen Energy 2019; 44(2):661-73.
31- Aranda-Jaramillo B, García-Depraect O, Aguilar-Juárez O, Archundia-Amador M, León-Becerril E. Continuous hydrogen production from cheese whey in a single-stage lactate-driven dark fermentation reactor. Journal of the Taiwan Institute of Chemical Engineers 2025: 106307. Doi: 10. 1016 / j. jtice.2025.106307.
32- Prakash NS, Maurer P, Horn H, Hille-Reichel A. Valorization of organic carbon in primary sludge via semi-continuous dark fermentation: First step to establish a wastewater biorefinery. Bioresource Technology 2024; 397:130467. Doi: 10. 1016/ j. biortech.2024.130467.
33- Ahmadi H, Jalil A, Khan S, Fabrice N, Zhang C, Yu Z. Optimized biohydrogen production from sewage sludge: Advanced pretreatment strategies in dark fermentation and microbial electrolysis cells. Energy Nexus 2025; 100573. Doi: 10. 1016/ j. nexus. 2025. 100573.
34- Gottardo M, Dosta J, Cavinato C, Crognale S, Tonanzi B, Rossetti S, et al. Boosting butyrate and hydrogen production in acidogenic fermentation of food waste and sewage sludge mixture: A pilot scale demonstration. Journal of Cleaner Production 2023; 404:136919. Doi: 10. 1016 /j. jclepro. 2023. 136919.
35- Kotay SM, Das D. Novel dark fermentation involving bioaugmentation with constructed bacterial consortium for enhanced biohydrogen production from pretreated sewage sludge. International Journal of Hydrogen Energy 2009; 34(17):7489-96.
36- Yu Z, Leng X, Zhao S, Ji J, Zhou T, Khan A, et al. A review on the applications of microbial electrolysis cells in anaerobic digestion. Bioresource Technology 2018; 255:340-48.
37- Li M, Zhang Q, Liu Y, Zhu J, Sun F, Cui MH, et al. Enhancing degradation of organic matter in microbial electrolytic cells coupled with anaerobic digestion (MEC-AD) systems by carbon-based materials. Science of the Total Environment 2023; 900:165805. Doi: 10. 1016/ j. scitotenv. 2023.165805.
38- Garduño Ibarra IR, Tsitouras A, Tanga S, Kinsley C, Baranova E. Electrochemically assisted dark fermentation for enhanced Hydrogen and Butyric Acid production from brewery waste slurry. Bioresource Technology Reports 2025; 102238. Doi: 10. 1016/ j. biteb. 2025. 102238.
39- Cabeza C, van Lier JB, Van Der Steen P. Effects of thermal and enzymatic pre-treatments on the solubilisation of extracellular polymeric substances (EPS) and subsequent anaerobic digestion of microalgae-bacterial biomass. Algal Research 2023; 72:103130. Doi: 10.1016/j.algal.2023.10313040.
40- Zhang J, Li N, Dai X, Tao W, Jenkinson IR, Li Z. Enhanced dewaterability of sludge during anaerobic digestion with thermal hydrolysis pretreatment: New insights through structure evolution. Water Research 2018; 131:177-85. Doi: 10. 1016/ j. watres. 2017. 12. 042.
41- Chen X, Huang J, Zhao J, Hu X, Zhang J, Yu X, et al. Destruction of sludge floc and extracellular polymeric substances structure by natural deep eutectic solvent with thermal treatment assistant for synergistic promotion of dewaterability and protein recovery from waste-activated sludge. Journal of Environmental Chemical Engineering 2025; 13(3):116702. Doi: 10.1016/j.jece.2025.116702.
42- Wei Y, Zhou Y, Wang S, Chen H. Understanding the mechanism of thermal hydrolysis to improve sludge dewaterability based on surface thermodynamics and XDLVO theory. Journal of Water Process Engineering 2024; 68:106514. Doi: 10. 1016/ j. jece. 2025. 116702.
43- Shi X, Zhu L, Li B, Liang J, Li XY. Surfactant-assisted thermal hydrolysis off waste activated sludge for improved dewaterability, organic release, and volatile fatty acid production. Waste Management 2021; 124:339-47.
44- Wu B, Su L, Dai X, Chai X. Development of montmorillonite-supported nano CaO2 for enhanced dewatering of waste-activated sludge by synergistic effects of filtration aid and peroxidation. Chemical Engineering Journal 2017; 307:418-26.
- چکیده مشاهده شده: 49 بار