Association of rs2910164, rs57095329 polymorphisms in miR146a and rs11614913 polymorphisms in miR196a2 with susceptibility to type 2 diabetes
Archives of Advances in Biosciences,
Vol. 16 No. 1 (2025),
2 March 2025
,
Page 1-8
https://doi.org/10.22037/aab.v16i1.50901
Abstract
Background and Aim: Studies have confirmed the significance of microRNAs (miRNAs) in various biological processes. Various miRNAs have been studied in diabetes mellitus, among them miR-146a and miR-196a2 play an important role in the development of this disease. This research aims to examine the frequency of rs2910164, rs57095329 polymorphisms in miR-146a and rs11614913 in miR-196a2, T2DM patient comparing to control group.
Methods: One hundred patients with T2DM and 100 healthy individuals as normal control group were included in this study. Polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) was exploited for genotyping of rs2910164C>G and rs11614913C>T and amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) was used for rs57095329A>G. Genotypes frequencies were evaluated with chi square test.
Results: We found that the GG genotype rs2910164 is significantly associated with T2DM susceptibility (OR = 12.1, 95% CI = 1.36-7.9, P = 0.003). Another investigation showed a significant association between the TT genotype of rs11614913 polymorphism and T2DM susceptibility (OR = 4.36, 95% CI = 1.13-5.84, P = 0.006). The GG genotype of rs57095329 showed a significant difference between T2DM and controls (OR = 8.37, 95% CI = 3.07 - 22.82, P < 0.0001).
Conclusion: To sum up, the study found that three genetic variants, rs2910164C>G, rs11614913C>T, and rs57095329A>G, were associated with an increased risk of type 2 diabetes mellitus (T2DM) in the Iranian population. These findings suggest that these variants may be new risk factors in the development of T2DM.
- type 2 diabetes
- miR196a2
- miR146a
- polymorphism
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References
6. References
1. Sakran N, Graham Y, Pintar T, Yang W, Kassir R, Willigendael EM, et al. The many faces of diabetes. Is there a need for re-classification? a narrative review. BMC Endocrine Disorders. 2022 Jan;22(1):9. (DOI: 10.1186/s12902-021-00927-y) (PMID)
2. Camaya I, Donnelly S, O'Brien B. Targeting the PI3K/Akt signaling pathway in pancreatic β-cells to enhance their survival and function: an emerging therapeutic strategy for type 1 diabetes. J Diabetes. 2022 Apr;14(4):247-60. (DOI: 10.1111/1753-0407.13252) (PMID)
3. Sun H, Saeedi P, Karuranga S, Pinkepank M, Ogurtsova K, Duncan BB, et al. IDF diabetes atlas: global, regional and country-level diabetes prevalence estimates for 2021 and projections for 2045. Diabetes Research and Clinical Practice. 2022 Jan;183:109119. (DOI: 10.1016/j.diabres.2021.109119) (PMID)
4. Vasbinder A, Anderson E, Shadid H, Berlin H, Pan M, Azam TU, et al. Inflammation, hyperglycemia, and adverse outcomes in individuals with diabetes mellitus hospitalized for COVID-19. Diabetes Care. 2022 Mar;45(3):692-700. (DOI: 10.2337/dc21-2102) (PMID)
5. Chan JCN, Lim LL, Wareham NJ, Shaw JE, Orchard TJ, Zhang P, et al. The Lancet Commission on diabetes: using data to transform diabetes care and patient lives. Lancet. 2021 Dec;396(10267):2019-82. (DOI: 10.1016/S0140-6736(20)32374-6) (PMID)
6. Alipour M, Malihi R, Hosseini SA, Abbasnezhad A, Ghavami A, Shahmohammadi HA, et al. The effects of catechins on related risk factors with type 2 diabetes: a review. Progress in Nutrition. 2018 Jan;20(1):12-20. (DOI:10.23751/pn.v20i1.6228)
7. Fuchsberger C, Flannick J, Teslovich TM, Mahajan A, Agarwala V, Gaulton KJ, et al. The genetic architecture of type 2 diabetes. Nature. 2016 Aug;536(7614):41-7. (DOI: 10.1038/nature18642) (PMID)
8. Panarelli NC, Yantiss RK. MicroRNA expression in selected carcinomas of the gastrointestinal tract. Pathology Research International. 2011 Feb;2011:124608. (DOI: 10.4061/2011/124608) (PMID)
9. Vasudevan S, Tong Y, Steitz JA. Switching from repression to activation: microRNAs can up-regulate translation. Science. 2007 Dec;318(5858):1931-4. (DOI: 10.1126/science.1149460) (PMID)
10. Chang H, Chang H, Cheng T, Lee GD, Chen X, Qi K. Micro-ribonucleic acid-23a-3p prevents the onset of type 2 diabetes mellitus by suppressing the activation of nucleotide-binding oligomerization-like receptor family pyrin domain containing 3 inflammatory bodies-caused pyroptosis through negatively regulating NIMA-related kinase 7. Journal of Diabetes Investigation. 2021 Mar;12(3):334-45. (DOI: 10.1111/jdi.13396) (PMID)
11. He C, Zhang Q, Sun H, Cai R, Pang W. Role of miRNA and lncRNA in animal fat deposition-a review. Sheng Wu Gong Cheng Xue Bao. 2020 Aug;36(8):1504-14. (DOI: 10.13345/j.cjb.200232) (PMID)
12. Guo R, Yu Y, Zhang Y, Li Y, Chu X, Lu H, et al. Overexpression of miR-297b-5p protects against stearic acid-induced pancreatic β-cell apoptosis by targeting LATS2. American Journal of Physiology-Endocrinology and Metabolism. 2020 Mar;318(3):E430-e9. (DOI: 10.1152/ajpendo.00302.2019) (PMID)
13. Wang SS, Wang C, Chen H. MicroRNAs are critical in regulating smooth muscle cell mineralization and apoptosis during vascular calcification. Journal of Cellular and Molecular Medicine. 2020 Dec;24(23):13564-72. (DOI: 10.1111/jcmm.16005) (PMID)
14. Huang Q, Chen H, Xu F, Liu C, Wang Y, Tang W, et al. Relationship of microRNA locus with type 2 diabetes mellitus: a case–control study. Endocrine Connections. 2021 Nov;10(11):1393-402. (DOI: 10.1530/EC-21-0261) (PMID)
15. Le Quesne J, Caldas C. Micro-RNAs and breast cancer. Molecular Oncology. 2010 Jun;4(3):230-41. (DOI: 10.1016/j.molonc.2010.04.009) (PMID)
16. Vaishya S, Sarwade RD, Seshadri V. MicroRNA, proteins, and metabolites as novel biomarkers for prediabetes, diabetes, and related complications. Frontiers in Endocrinology. 2018 Apr;9:180. (DOI: 10.3389/fendo.2018.00180) (PMID: 29740397)
17. Padilla-Martinez F, Wojciechowska G, Szczerbinski L, Kretowski A. Circulating nucleic acid-based biomarkers of type 2 diabetes. International Journal of Molecular Sciences. 2022 Dec;23(1):295. (DOI: 10.3390/ijms23010295) (PMID: 35008723)
18. Gan WZ, Ramachandran V, Lim CSY, Koh RY. Omics-based biomarkers in the diagnosis of diabetes. Journal of Basic and Clinical Physiology and Pharmacology. 2019 Nov;31(2). (DOI: 10.1515/jbcpp-2019-0120) (PMID)
19. Fichtlscherer S, De Rosa S, Fox H, Schwietz T, Fischer A, Liebetrau C, et al. Circulating microRNAs in patients with coronary artery disease. Circulation Research. 2010 Sep;107(5):677-84. (DOI: 10.1161/CIRCRESAHA.109.215566) (PMID)
20. Ghaffari M, Razi S, Zalpoor H, Nabi-Afjadi M, Mohebichamkhorami F, Zali H. Association of MicroRNA-146a with type 1 and 2 diabetes and their related complications. Journal of Diabetes Research. 2023 Mar;2023:2587104. (DOI: 10.1155/2023/2587104) (PMID)
21. Alipoor B, Ghaedi H, Meshkani R, Torkamandi S, Saffari S, Iranpour M, et al. Association of MiR-146a expression and type 2 diabetes mellitus: a meta-analysis. International Journal of Molecular and Cellular Medicine. 2017 Aug;6(3):156-63. (DOI: 10.22088/acadpub.BUMS.6.3.156) (PMID)
22. Aziz MA, Akter T, Islam MS. Effect of miR-196a2 rs11614913 polymorphism on cancer susceptibility: evidence from an updated meta-analysis. Technology in Cancer Research and Treatment. 2022 Jan-Dec;21:15330338221109798. (DOI: 10.1177/15330338221109798) (PMID)
23. Shankaran ZS, Walter CEJ, Ramachandiran K, Gurramkonda VB, Johnson T. Association of microRNA-146a rs2910164 polymorphism with type II diabetes mellitus in a South Indian population and a meta-analysis. Gene Reports. 2020 Mar;18:100567. (DOI: 10.1016/j.genrep.2019.100567)
24. Alipoor B, Meshkani R, Ghaedi H, Sharifi Z, Panahi G, Golmohammadi T. Association of miR-146a rs2910164 and miR-149 rs2292832 variants with susceptibility to type 2 diabetes. Clinical Laboratory. 2016 Aug;62(8):1553-61. (DOI: 10.7754/Clin.Lab.2016.160124) (PMID)
25. Ciccacci C, Di Fusco D, Cacciotti L, Morganti R, D'Amato C, Greco C, et al. MicroRNA genetic variations: association with type 2 diabetes. Acta Diabetologica. 2013 Dec;50(6):867-72. (DOI: 10.1007/s00592-013-0469-7) (PMID)
26. Ibrahim AA, Ramadan A, Wahby AA, Hassan M, Soliman HM, Abdel Hamid TA. Micro-RNA 196a2 expression and miR-196a2 (rs11614913) polymorphism in T1DM: a pilot study. Journal of Pediatric Endocrinology and Metabolism. 2019 Oct;32(10):1171-9. (DOI: 10.1515/jpem-2019-0226) (PMID)
27. Wang TT, Chen YJ, Sun LL, Zhang SJ, Zhou ZY, Qiao H. Affection of single-nucleotide polymorphisms in miR-27a, miR-124a, and miR-146a on susceptibility to type 2 diabetes mellitus in Chinese Han people. Chinese Medical Journal. 2015 Feb;128(04):533-9. (DOI: 10.4103/0366-6999.151112) (PMID)
28. Donath MY, Shoelson SE. Type 2 diabetes as an inflammatory disease. Nature Reviews Immunology. 2011 Feb;11(2):98-107. (DOI: 10.1038/nri2925) (PMID)
29. Sankaran PP, Prajeeth RN, Koteshwara P. Association of vascular calcification of breast with carotid intima media thickness. Polish Journal of Radiology. 2019 Aug;84:e335-e9. (DOI: 10.5114/pjr.2019.88064) (PMID)
30. Cheng L, Zhou M, Zhang D, Chen B. Association of miR-146a polymorphism rs2910164 and type 2 diabetes risk: a meta-analysis. Journal of International Medical Research. 2020 Aug;48(8):0300060520931313. (DOI: 10.1177/0300060520931313) (PMID)
31. Li Y, Zhang Y, Li X, Shi L, Tao W, Shi L, et al. Association study of polymorphisms in miRNAs with T2DM in Chinese population. International Journal of Medical Sciences. 2015 Oct;12(11):875-80. (DOI: 10.7150/ijms.12954) (PMID)
32. Yong R, Chen XM, Shen S, Vijayaraj S, Ma Q, Pollock CA, et al. Plumbagin ameliorates diabetic nephropathy via interruption of pathways that include NOX4 signalling. PLOS One. 2013 Aug;8(8):e73428. (DOI: 10.1371/journal.pone.0073428) (PMID)
33. Lu Y, Cao DL, Jiang BC, Yang T, Gao YJ. MicroRNA-146a-5p attenuates neuropathic pain via suppressing TRAF6 signaling in the spinal cord. Brain, Behavior, and Immunity. 2015 Oct;49:119-29. (DOI: 10.1016/j.bbi.2015.04.018) (PMID)
34. Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, et al. A mammalian microRNA expression atlas based on small RNA library sequencing. Cell. 2007 Jun;129(7):1401-14. (DOI: 10.1016/j.cell.2007.04.040) (PMID)
35. Yin Z, Cui Z, Ren Y, Xia L, Li H, Zhou B. MiR-196a2 and lung cancer in Chinese non-smoking females: a genetic association study and expression analysis. Oncotarget. 2017 Aug;8(41):70890-8. (DOI: 10.18632/oncotarget.20174) (PMID)
36. Zhao H, Xu J, Zhao D, Geng M, Ge H, Fu L, et al. Somatic mutation of the SNP rs11614913 and its association with increased MIR 196A2 expression in breast cancer. DNA Cell Biology. 2016 Feb;35(2):81-7. (DOI: 10.1089/dna.2014.2785) (PMID)
37. Ghanbari M, Sedaghat S, de Looper HW, Hofman A, Erkeland SJ, Franco OH, et al. The association of common polymorphisms in miR-196a2 with waist to hip ratio and miR-1908 with serum lipid and glucose. Obesity (Silver Spring). 2015 Feb;23(2):495-503. (DOI: 10.1002/oby.20975) (PMID)
38. Yin Z, Cui Z, Guan P, Li X, Wu W, Ren Y, et al. Interaction between polymorphisms in Pre-MiRNA genes and cooking oil fume exposure on the risk of lung cancer in Chinese non-smoking female population. PLOS One. 2015 Jun;10(6):e0128572. (DOI: 10.1371/journal.pone.0128572) (PMID)
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