Multidrug Resistance Patterns among Clinical Isolates of Acinetobacter Baumannii
Archives of Advances in Biosciences,
Vol. 16 No. 1 (2025),
2 March 2025
,
Page 1-8
https://doi.org/10.22037/aab.v16i1.50782
Abstract
Background and Aim: Acinetobacter baumannii is a major cause of hospital‑acquired infections and exhibits increasing multidrug resistance (MDR). This study aimed to determine antibiotic resistance patterns and the prevalence of MDR and XDR phenotypes among clinical isolates from a tertiary‑care hospital in Tehran.
Methods: Clinical isolates were collected over 12 months. Identification was performed using standard biochemical tests and PCR (blaOXA‑51). Antimicrobial susceptibility testing was conducted using the CLSI‑standardized Kirby–Bauer disk diffusion method. MDR and XDR were defined according to international criteria.
Results: High resistance was observed to carbapenems and cephalosporins. Colistin remained the most effective
e agent. Overall, 92% of isolates were MDR and 47% were XDR.
Conclusion: MDR and XDR A. baumannii strains were widely prevalent, indicating an urgent need for antibiotic stewardship and routine surveillance.
- Acinetobacter baumannii
- multidrug resistance
- carbapenem resistance
- hospital acquired infection
How to Cite
References
1. Foglia F, Ambrosino A, Bashir S, Finamore E, Zannella C, Donnarumma G, De Filippis A, Galdiero M. Prevalence of Acinetobacter baumannii Multidrug Resistance in University Hospital Environment. Antibiotics. 2025 May 10;14(5):490.(PMID)
2. Ghamari M, Jabalameli F, Afhami S, Halimi S, Emaneini M, Beigverdi R. Acinetobacter baumannii infection in critically ill patients with COVID-19 from Tehran, Iran: the prevalence, antimicrobial resistance patterns and molecular characteristics of isolates. Frontiers in Cellular and Infection Microbiology. 2025 Jan 30;14:1511122.(LINK)
3. Borcan AM, Rotaru E, Caravia LG, Filipescu MC, Simoiu M. Trends in Antimicrobial Resistance of Acinetobacter baumannii and Pseudomonas aeruginosa from Bloodstream Infections: An Eight-Year Study in a Romanian Tertiary Hospital. Pharmaceuticals. 2025 Jun 24;18(7):948.(LINK)
4. Emamgholitabar Malekshah F, Gholami M, Pahlavanian M, Goli HR. Investigating the prevalence of class 1, 2, and 3 integrons in carbapenem-resistant Acinetobacter baumannii isolated from burn wound infections. Scientific Reports. 2025 Aug 29;15(1):31857. (LINK)
5. Sánchez-Urtaza S, Alfonso-Alarcón L, Arazo del Pino R, Burgwinkel T, Ocampo-Sosa A, Gonzalez R, Xanthopoulou K, Higgins PG, Alkorta I, Gallego L. Genomic characterization of multidrug-resistant clinical Acinetobacter baumannii isolates from a hospital in Paraguay. Frontiers in Cellular and Infection Microbiology. 2025 Jul 31;15:1620479.(LINK)
6. Kebriaee AN, Behzadi P, Mohammadian T, Imani Fooladi AA, Hosseini MS. Distribution of antimicrobial resistance genes and virulence genes in different genotypes of Acinetobacter baumannii. BMC Infectious Diseases. 2025 Oct 2;25(1):1230.(PMID)
7. Goncalves EK, Penwell WF, Fiester SE. Combating the Growing Threat of Acinetobacter baumannii Resistance. Antibiotics. 2025 Jul 9;14(7):694.(LINK)
8. Javadi K, Ghaemian P, Baziboron M, Pournajaf A. Investigating the Link Between Biofilm Formation and Antibiotic Resistance in Clinical Isolates of Acinetobacter baumannii. International Journal of Microbiology. 2025;2025(1):1009049.(PMID)
9. Yamada AY, de Souza AR, Madalosso G, de Assis DB, França FA, Santos MB, Campos KR, Sacchi CT, Tiba-Casas MR, Carvalho E, Camargo CH. Emergence of carbapenem-resistant Acinetobacter baumannii clonal complex 2 in multiple hospitals in São Paulo state, Brazil. Antimicrobial Agents and Chemotherapy. 2025 Sep 3;69(9):e01865-24. (PMID)
10. Najafi-Olya Z, Heydarifard Z, Looha MA, Ahmadi AS, Yarhamadi N, Safaei M. Antibiotic resistance and bacterial co-infections in COVID-19 patients in Iran: a systematic review and meta-analysis of hospitalized and non-hospitalized cases. BMC infectious diseases. 2025 Sep 29;25(1):1197.(PMID)
11. Shahari AS, Palanisamy NK, Mohd Nor F. Genetic profiling of multidrug-resistant Acinetobacter baumannii from a tertiary care center in Malaysia. Microbiology Spectrum. 2025 Feb 4;13(2):e00872-24. (PMID)
12. Hematian A, Karami-Zarandi M, Rahdar HA, Heidari H, Kazemian H. Investigation of colistin heteroresistance in multidrug-resistant Acinetobacter baumannii clinical isolates. New Microbes and New Infections. 2025 Aug 29:101633.(PMID)
13. Hafiz TA, Alghamdi SS, Mubaraki MA, Alghamdi SS, Alothaybi A, Aldawood E, Alotaibi F. A two-year retrospective study of multidrug-resistant Acinetobacter baumannii respiratory infections in critically Ill patients: Clinical and microbiological findings. Journal of Infection and Public Health. 2023 Mar 1;16(3):313-9.(PMID)
14. Elshamy AA, Kamal SK, Mahmoud MT, Elhasany AM, Shady AA, Mohamed SA, Abd-Elmaaboud HA, El-Awady NE, Mohamed RA, El-Mirghany SA, El-Hady SW. Recent insights on phage therapy against multidrug-resistant Acinetobacter baumannii. AMB Express. 2025 Dec;15(1):1-6.(PMID)
15. Mottaghiyan Z, Esmaeili D, Ahmadi MH, Niakan. Development of a Multiplex PCR Assay for the Detection of Extended-Spectrum Beta-Lactamase Genes in Acinetobacter Baumannii Isolates in Tehran City, Iran. Indian Journal of Microbiology. 2024 Sep;64(3):910-6.(PMID)
16. Amini MS, Baseri Salehi M, Bahador N. Evaluating the antibacterial effect of meropenem-loaded chitosan/sodium tripolyphosphate (TPP) nanoparticles on Acinetobacter baumannii isolated from hospitalized patients. BMC Infectious Diseases. 2024 Jun 24;24(1):631.(PMID)
17. Sezavar M, Soheili V, Mahnia Z, Moghaddam AB, Fazli B. Perspectives on the fighting against Acinetobacter baumannii via plants as promising weapons against multidrug-resistant bacteria: A Systematic Review. Journal of Herbal Medicine. 2025 Apr 29:101025.(LINK)
18. Asmare Z, Tamrat E, Erkihun M, Endalamaw K, Alelign D, Getie M, Sisay A, Gashaw Y, Reta MA. Antimicrobial resistance pattern of Acinetobacter baumannii clinical isolate in Ethiopia. A systematic review and meta-analysis. BMC Infectious Diseases. 2025 Apr 12;25(1):518. (PMID)
19. Khoshnood S, Sadeghifard N, Mahdian N, Heidary M, Mahdian S, Mohammadi M, Maleki A, Haddadi MH. Antimicrobial resistance and biofilm formation capacity among Acinetobacter baumannii strains isolated from patients with burns and ventilator‐associated pneumonia. Journal of clinical laboratory analysis. 2023 Jan;37(1):e24814.(LINK)
20. Kyriakidis I, Vasileiou E, Pana ZD, Tragiannidis A. Acinetobacter baumannii antibiotic resistance mechanisms. Pathogens. 2021 Mar 19;10(3):373.(LINK)
21. Lee CR, Lee JH, Park M, Park KS, Bae IK, Kim YB, Cha CJ, Jeong BC, Lee SH. Biology of Acinetobacter baumannii: pathogenesis, antibiotic resistance mechanisms, and prospective treatment options. Frontiers in cellular and infection microbiology. 2017 Mar 13;7:55. (LINK)
22. Rodríguez CH, Balderrama Yarhui N, Nastro M, Nuñez Quezada T, Castro Cañarte G, Magne Ventura R, Ugarte Cuba T, Valenzuela N, Roach F, Mota MI, Burger N. Molecular epidemiology of carbapenem-resistant Acinetobacter baumannii in South America. Journal of Medical Microbiology. 2016 Oct 18;65(10):1088-91.(LINK)
23. Melgarejo-Touchet N, Brítez CM, Busignani S, Falcón M, López E, Laconich M, Blasco R, Arguello R, Kawabata A, Olmedo M, Rojas C. Caracterización molecular de carbapenemasas en bacilos gramnegativos circulantes en hospitales de Paraguay. Primer cuatrimestre 2021. Memorias del Instituto de Investigaciones en Ciencias de la Salud. 2021 Aug 1;19(2):49-58.(LINK)
24. Howard A, O’Donoghue M, Feeney A, Sleator RD. Acinetobacter baumannii: an emerging opportunistic pathogen. Virulence. 2012 May 1;3(3):243-50.(PMID)
25. Lucien MA, Canarie MF, Kilgore PE, Jean-Denis G, Fénélon N, Pierre M, Cerpa M, Joseph GA, Maki G, Zervos MJ, Dely P. Antibiotics and antimicrobial resistance in the COVID-19 era: Perspective from resource-limited settings. International journal of infectious diseases. 2021 Mar 1;104:250-4.(PMID)
26. Weinberg SE, Villedieu A, Bagdasarian N, Karah N, Teare L, Elamin WF. Control and management of multidrug resistant Acinetobacter baumannii: A review of the evidence and proposal of novel approaches. Infection Prevention in Practice. 2020 Sep 1;2(3):100077.(PMID)
27. García-Quintanilla M, Pulido MR, López-Rojas R, Pachón J, McConnell MJ. Emerging therapies for multidrug resistant Acinetobacter baumannii. Trends in microbiology. 2013 Mar 1;21(3):157-63.(PMID)
28. Chen LK, Chang JC, Chu HT, Chen YT, Jiang HL, Wang LS, Teh SH, Yang HH, Chen DS, Li YZ, Chang CC. Preoptimized phage cocktail for use in aerosols against nosocomial transmission of carbapenem-resistant Acinetobacter baumannii: A 3-year prospective intervention study. Ecotoxicology and environmental safety. 2022 May 1;236:113476. (PMID)
29. Li S, Wei B, Xu L, Cong C, Murtaza B, Wang L, Li X, Li J, Xu M, Yin J, Xu Y. A novel Saclayvirus Acinetobacter baumannii phage genomic analysis and effectiveness in preventing pneumonia. Applied Microbiology and Biotechnology. 2024 Dec;108(1):428.(PMID)
30. Wang Z, Yang X, Wang H, Wang S, Fang R, Li X, Xing J, Wu Q, Li Z, Song N. Characterization and efficacy against carbapenem-resistant Acinetobacter baumannii of a novel Friunavirus phage from sewage. Frontiers in Cellular and Infection Microbiology. 2024 Apr 22;14:1382145.(PMID)
31. Banoub NG, Saleh SE, Helal HS, Aboshanab KM. Antibiotics combinations and chitosan nanoparticles for combating multidrug resistance Acinetobacter baumannii. Infection and Drug Resistance. 2021 Aug 20:3327-39. (PMID)
32. Vrancianu CO, Gheorghe I, Czobor IB, Chifiriuc MC. Antibiotic resistance profiles, molecular mechanisms and innovative treatment strategies of Acinetobacter baumannii. Microorganisms. 2020 Jun 21;8(6):935.(PMID)
33. Joshi SG, Litake GM. Acinetobacter baumannii: An emerging pathogenic threat to public health. World Journal of Clinical Infectious Diseases. 2013 Aug 25;3(3):25-36.(LINK)
34. Benaissa E, Maleb A, Elouennass M. Antimicrobial resistance of multidrug-resistant Enterobacterales and Acinetobacter baumannii isolates to colistin in a Moroccan hospital. Microbes and Infectious Diseases. 2024 Aug 1;5(3):1139-46. (LINK)
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