The Effects of Trans-anethole on the Hypothalamic CGRP and CRH Gene Expression in Rat Model of Stress
Archives of Advances in Biosciences,
Vol. 14 No. 1 (2023),
19 February 2023
,
Page 1-7
https://doi.org/10.22037/aab.v14i1.41158
Abstract
Introduction: Stress is defined as a physiological response to environmental conditions which could cause changes in the level of neuropeptides in the central nervous system. Trans- anethole is the secondary active compound with anti-stress and antioxidant properties. This research investigates the effects of trans-anethole on the hypothalamic CRH and CGRP gene expression in stress model rats.
Materials and Methods: Twenty male rats weighing 200-220 g were used. Animals were divided into four groups (n=5). The intact control or stress groups received saline. Two stress groups received trans-anethole (150 mg/kg or 250 mg/kg, IP). Thirty minutes following the injection of drugs, animals were subjected to acute immobilization stress for two hours. Then, behavioral tests were performed. The hypothalamic samples were removed. CRH and CGRP gene expression was measured using RT-PCR.
Results: The mRNA levels of CGRP and CRH significantly increased in the stress group compared to those of the control. In rats receiving 150 mg/kg or 250 mg/kg of trans-anethole, the mRNA level of CGRP and CRH decreased significantly compared to that of the stress group. Also, injection of 150 mg/kg or 250 mg/kg of trans-anethole significantly improved the stressful behaviors compared to what happened in the stress group.
Conclusion: Trans-anethole may be considered as a potential anti-stress factor due to its inhibitory effects on the activity of hypothalamic stress pathways such as CRH and CGRP.
- Stress
- Trans anethole
How to Cite
References
Williams JL, Everett JM, D’Cunha NM, Sergi D, Georgousopoulou EN, Keegan RJ, et al. The effects of green tea amino acid L-theanine consumption on the ability to manage stress and anxiety levels: A systematic review. Plant Foods Hum Nutr. 2020; 75:12-23. [DOI:10.1007/s11130-019-00771-5] [PMID]
Chrousos, G. Stress and disorders of the stress system. Nature reviews endocrinology 2009; 5(7):374-81. [DOI:10.1038/nrendo.2009.106] [PMID]
Cool J, Zappetti D. The physiology of stress. Medical Student Well-Being: An Essential Guide; 2019. https://link.springer.com/chapter/10.1007/978-3-030-16558-1_1
Yule LR, Garelja ML, Hendrikse ER, Gingell JJ, Poyner DR, Harris PW, et al. A potent fluorescent calcitonin gene‐related peptide analogue enables visualization of receptor internalization. Peptide Sci. 2019; 111(6):e24126. [DOI:10.1002/pep2.24126]
Singh Y, Gupta G, Shrivastava B, Dahiya R, Tiwari J, Ashwathanarayana M, et al. Calcitonin gene‐related peptide (CGRP): A novel target for Alzheimer's disease. CNS Neurosci Ther. 2017; 23(6):457-61. [DOI:10.1111/cns.12696] [PMID] [PMCID]
Vegas O, Poligone B, Blackcloud P, Gilmore ES, VanBuskirk J, Ritchlin CT, et al. Chronic social stress Ameliorates psoriasiform dermatitis through upregulation of the Hypothalamic-Pituitary-Adrenal axis. Brain Behav Immun. 2018; 68:238-47. [DOI:10.1016/j.bbi.2017.10.022] [PMID] [PMCID]
Wei P, Keller C, Li L. Neuropeptides in gut-brain axis and their influence on host immunity and stress. Comput Struct Biotechnol J. 2020; 18:843-51. [DOI:10.1016/j.csbj.2020.02.018] [PMID] [PMCID]
Jiang Z, Tong Q. Hypothalamic CRH neurons: a crossroad between stress and metabolism. Curr Opin Endocr Metab Res. 2022; 26:100384. [DOI:10.1016/j.coemr.2022.100384]
Al-Snafi AE. The chemical constituents and pharmacological effects of Foeniculum vulgare-A review. IOSR J Pharm. 2018; 8(5):81-96. https://www.researchgate.net/publication/325809622_The_chemical_constituents_and_pharmacological_effects_of_Foeniculum_vulgare-A_review
Ateş DA, Turgay Ö. Antimicrobial activities of various medicinal and commercial plant extracts. Turk J Biol. 2003; 27(3):157-62. https://journals.tubitak.gov.tr/cgi/viewcontent.cgi?article=2183&context=biology
Mahboubi M. Foeniculum vulgare as valuable plant in management of women's health. J Menopausal Med. 2019; 25(1):1-14. [DOI:10.6118/jmm.2019.25.1.1] [PMID] [PMCID]
Moradi J, Abbasipour F, Zaringhalam J, Maleki B, Ziaee N, Khodadoustan A, et al. Anethole, a medicinal plant compound, decreases the production of pro-inflammatory TNF-α and IL-1β in a rat model of LPS-induced periodontitis. Iran J Pharm Res. 2014; 13(4):1319. [PMID] [PMCID]
Kang X, Hong W, Xie K, Tang H, Tang J, Luo S, et al. Ginsenoside Rb1 pretreatment reverses hippocampal changes in BDNF/TrkB mRNA and protein in rats subjected to acute immobilization stress. Drug Des Devel Ther. 2019; 13:2127-34. [DOI:10.2147/DDDT.S201135] [PMID] [PMCID]
Urakawa S, Takamoto K, Hori E, Sakai N, Ono T, Nishijo H. Rearing in enriched environment increases parvalbumin-positive small neurons in the amygdala and decreases anxiety-like behavior of male rats. BMC Neurosci. 2013; 14:1-13. [DOI:10.1186/1471-2202-14-13]
Iñiguez SD, Parise LF, Lobo MK, Flores-Ramirez FJ, Garcia-Carachure I, Warren BL, et al. Upregulation of hippocampal extracellular signal-regulated kinase (ERK)–2 induces antidepressant-like behavior in the rat forced swim test. Behav. Neurosci. 2019; 133(2):225-31. [DOI:10.1037/bne0000303]
Mahmoudi F, Haghighat Gollo K. Influences of serotonin hydrochloride on adiponectin, ghrelin and kiss1 genes expression. Galen Med J. 2020; 9:e1767. [DOI: 10.31661/gmj.v9i0.1767] [PMID] [PMCID]
Miyagawa M, Satou T, Yukimune C, Ishibashi A, Seimiya H, Yamada H, et al. Anxiolytic‐like effect of illicium verum fruit oil, trans‐anethole and related compounds in mice. Phytother Res. 2014; 28(11):1710-2. [DOI:10.1002/ptr.5190] [PMID]
Claes S. Corticotropin‐releasing hormone (CRH) in psychiatry: from stress to psychopathology. Ann Med. 2004; 36(1):50-61. [DOI:10.1080/07853890310017044] [PMID]
Dunčko R, Kiss A, Škultétyová I, Rusnák M, Ježová D. Corticotropin-releasing hormone mRNA levels in response to chronic mild stress rise in male but not in female rats while tyrosine hydroxylase mRNA levels decrease in both sexes. Psychoneuroendocrinology. 2001; 26(1):77-89. [DOI:10.1016/s0306-4530(00)00040-8] [PMID]
Sink KS, Walker DL, Yang Y, Davis M. Calcitonin gene-related peptide in the bed nucleus of the stria terminalis produces an anxiety-like pattern of behavior and increases neural activation in anxiety-related structures. J Neurosci. 2011; 31(5):1802-10. [DOI:10.1523/JNEUROSCI.5274-10.2011] [PMID] [PMCID]
Alireza K, Faeghe H, Siamak S, Negar B. Study of the effect of extract of Thymus vulgaris on anxiety in male rats. J Tradit Complement Med. 2015; 6(3):257-61. [DOI:10.1016/j.jtcme.2015.01.001] [PMID]
Sulakhiya K, Patel VK, Saxena R, Dashore J, Srivastava AK, Rathore M. Effect of Beta vulgaris Linn. leaves extract on anxiety-and depressive-like behavior and oxidative stress in mice after acute restraint stress. Pharmacognosy Res. 2016; 8(1):1. [DOI:10.4103/0974-8490.171100] [PMID] [PMCID]
Samad N, Saleem A. Administration of Allium cepa L. bulb attenuates stress-produced anxiety and depression and improves memory in male mice. Metab Brain Dis. 2018; 33:271-81. [DOI:10.1007/s11011-017-0159-1] [PMID]
Walf AA, Frye CA. Estradiol decreases anxiety behavior and enhances inhibitory avoidance and gestational stress produces opposite effects. Stress. 2007; 10(3):251-60. [DOI:10.1080/00958970701220416] [PMID]
Ni X, Nicholson RC, King BR, Chan EC, Read MA, Smith R. Estrogen represses whereas the estrogen-antagonist ICI 182780 stimulates placental CRH gene expression. J Clin Endocrinol Metab. 2002; 87(8):3774-8. [DOI:10.1210/jcem.87.8.8745] [PMID]
Goncharova ND. The HPA axis under stress and aging: individual vulnerability is associated with behavioral patterns and exposure time. Bioessays. 2020; 42(9):e2000007. [DOI:10.1002/bies.202000007] [PMID]
García-León MÁ, Pérez-Mármol JM, Gonzalez-Pérez R, del Carmen García-Ríos M, Peralta-Ramírez MI. Relationship between resilience and stress: Perceived stress, stressful life events, HPA axis response during a stressful task and hair cortisol. Physiol Behav. 2019; 202:87-93. [DOI:10.1016/j.physbeh.2019.02.001] [PMID]
Van Stegeren AH, Wolf OT, Everaerd W, Rombouts SA. Interaction of endogenous cortisol and noradrenaline in the human amygdala. Prog Brain Res. 2008; 167:263-8. [DOI:10.1016/S0079-6123(07)67020-4] [PMID]
Thompson BL, Erickson K, Schulkin J, Rosen JB. Corticosterone facilitates retention of contextually conditioned fear and increases CRH mRNA expression in the amygdala. Behav Brain Res. 2004; 149(2):209-15. [DOI:10.1016/s0166-4328(03)00216-x] [PMID]
Chaves T, Fazekas CL, Horváth K, Correia P, Szabó A, Török B, et al. Stress adaptation and the brainstem with focus on corticotropin-releasing hormone. Int J Mol Sci. 2021; 22(16):9090. [DOI:10.3390/ijms22169090] [PMID] [PMCID]
Carboni L, El Khoury A, Beiderbeck DI, Neumann ID, Mathe AA. Neuropeptide Y, calcitonin gene-related peptide, and neurokinin A in brain regions of HAB rats correlate with anxiety-like behaviours. Eur Neuropsychopharmacol. 2022; 57:1-4. [DOI:10.1016/j.euroneuro.2021.12.011] [PMID]
Sahraei H, Ghoshooni H, Salimi SH, Astani AM, Shafaghi B, Falahi M, et al. The effects of fruit essential oil of the Pimpinella anisum on acquisition and expression of morphine induced conditioned place preference in mice. J Ethnopharmacol. 2002; 80(1):43-7. [DOI:10.1016/s0378-8741(02)00012-0] [PMID]
Lee SE, Lee Y, Lee GH. The regulation of glutamic acid decarboxylases in GABA neurotransmission in the brain. Arch Pharm Res. 2019; 42:1031-9. [DOI:10.1007/s12272-019-01196-z] [PMID]
Bourgoin S, Pohl M, Benoliel JJ, Mauborgne A, Collin E, Hamon M, et al. gamma-Aminobutyric acid, through GABAA receptors, inhibits the potassium-stimulated release of calcitonin gene-related peptide- but not that of substance P-like material from rat spinal cord slices. Brain Res. 1992; 583(1-2):344-8. [DOI:10.1016/s0006-8993(10)80048-4] [PMID]
Marsland AL, Walsh C, Lockwood K, John-Henderson NA. The effects of acute psychological stress on circulating and stimulated inflammatory markers: a systematic review and meta-analysis. Brain Behav Immun. 2017; 64:208-19. [DOI:10.1016/j.bbi.2017.01.011] [PMID] [PMCID]
Averbeck B, Reeh PW. Interactions of inflammatory mediators stimulating release of calcitonin gene-related peptide, substance P and prostaglandin E2 from isolated rat skin. Neuropharmacology. 2001; 40(3):416-23. [DOI:10.1016/s0028-3908(00)00171-4] [PMID]
Negahdari FM, Gholamnezhad Z, Noshahr ZS, Keshavarzi Z. A comparison between the effect of trans-anethole and metformin on biochemical parameters of polycystic ovary syndrome in rats. Avicenna J Phytomed. 2021; 11(5):484-93. [DOI:10.22038/AJP.2021.55679.2785] [PMID] [PMCID]
Gharib R, Greige-Gerges H, Fourmentin S, Charcosset C. Hydroxypropyl-ß-cyclodextrin as a membrane protectant during freeze-drying of hydrogenated and non-hydrogenated liposomes and molecule-in-cyclodextrin-in-liposomes: Application to trans-anethole. Food Chem. 2018; 267:67-74. [DOI:10.1016/j.foodchem.2017.10.144] [PMID]
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