Survey of Environmental Bioaerosols in ICU ward of Taleghani Hospital in Tehran by Petri - dish trapping technique and Bioaerosol Sampler in 2013
Irtiqa Imini Pishgiri Masdumiyat (Safety Promotion and Injury Prevention),
Vol. 2 No. 2 (2014),
20 December 2014
,
Page 133-139
https://doi.org/10.22037/meipm.v2i2.7062
Abstract
Backgrounds and Objective:Bio aerosols are particles that have derived from microbes, viruses, fungi, pollen, that they can cause secondary hospital infections. This topic is important for patients with low immunity levels. The purpose of this study is compare two methods of passive and active environmental bio aerosol measurements in ICU Ayatollah Taleghani Hospital, Tehran.
Materials and Methods:This is a field-cross–sectional study; the sampling carried out by active and passive methods at 120 cm height above the floor, on a suitable culturemedium. The medium inboth methods was placed in incubator (37±20c) and after 24 or 48 hours, the number of colonies by microscopy were counted, then bacteria’s density based on cfu / m³ was calculated.
Results:in most cases (6/97%), Bacterial contamination in air of ICU was Gram-negative and growth of Gram-positive bacteria no observed or was very small (37/2%).Compare two passive and active sampling methods in whole time of sampling in different stations ,in view of colony number , Gram-positive bacteria was at A and B levels EU GMP standard (very clean and clean respectively ), and for Gram-negative bacteria often found in the C level EU GMPstandard (medium).
Conclusion:The results showed that the lowest and the highest colony growth are related to Gram-positive and Gram-negative bacteria respectively.Finally, it is suggested discharge the air by exhaust fans and avoided from opening windows.
How to cite this article: Massoudinejad MR, Ghajari A, Hezarkhani N, Aliyari A. Survey of Environmental Bioaerosols in ICU ward of Taleghani Hospital in Tehran by Petri-dish trapping technique and Bioaerosol Sampler in 2013. Irtiqa Imini Pishgiri Masdumiyat (Safety Promotion and Injury Prevention).2014;2(2):133-139.
- bio aerosol, Petri - dish trapping technique, Bio aerosol Sampler, Gram - positive and Gram - negative bacteria
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