Improvement of Probiotic Survival in Fruit juice and under Gastrointestinal conditions using Pectin-Nanochitin-Nanolignocellulose as a Novel Prebiotic Gastrointestinal-Resistant Matrix
Applied Food Biotechnology,
Vol. 4 No. 3 (2017),
21 June 2017
,
Page 179-191
https://doi.org/10.22037/afb.v4i3.17337
Abstract
Background and Objective: Increasing survivability of probiotics in low pH juices and in gastrointestinal conditions is important for probiotic food industry. Nanofibers can reinforce the structure of entrapment matrices protecting probiotics in harsh conditions. This study investigated pectin-based bionanocomposites improved with nanochitin, nanolignocellulose and bacterial nanocellulose to introduce a prebiotic gastrointestinal-resistant matrix for enhancing the survival of Bacillus coagulans as a probiotic.
Material and Methods: The bionanocomposites with various compositions were designed using mixture design method. These were fabricated based on cross-linking of calcium ions with pectin for entrapment of Bacillus coagulans. The survivability of probiotic was evaluated at 4°C or 25°C over a 5-week storage in peach juice and under simulated gastrointestinal conditions.
Results and Conclusion: The prebiotic score of the pectin-nanochitin-nanolignocellulose (50:25:25% w w-1) was determined as 1.36. The survivability of Bacillus coagulans entrapped within the pectin-nanochitin-nanolignocellulose matrix was ~65% under gastrointestinal treatment. The surface structure of the matrix was relatively smooth coherent, compact and wrinkled due to the three-dimensional arrangement of the nanofibers of chitin and lignocellulose incorporated within pectin. The highest survivability of the entrapped bacteria was ~68% compared to the survivability of the free cell (~53%) at the end of 5-week storage period. After 21 day storage in the juice, the survivability of the entrapped bacteria treated under sequential digestion was ~58% as compared to that of the free cell (~43%). The present findings proposed a promising prebiotic matrix to protect probiotics in low pH fruit juice and the gastrointestinal tract.
Conflict of interest: The authors declare no conflict of interest.
- ▪ Bacillus coagulans ▪ Nanochitin ▪ Nanolignocellulose ▪ Pectin ▪ Synbiotic juice
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References
Mollakhalili Meybodi N, Mortazavian AM, Sohrabvandi s, G da Cruz A, Mohammadi R. Probiotic supplements and food products: Comparison for different targets. Appl Food Biotechnol. 2017; 4(3): 123-132. doi: 10.22037/afb.v4i3-.16420
Eksiri M, Nateghi L, Rahmani A. Production of probiotic drink using Pussy willow and Echium amoenum extracts. Appl Food Biotechnol. 2017; 4(3): 155-165. doi: 10.22037/afb.v4i3-.16433
Malganji S, Sohrabvandi S, Jahadi M, Nematollahi A, Sarmadi B. Effect of refrigerated storage on sensory properties and viability of probiotic in grape drink. Appl Food Biotechnol. 2016; 3(1): 59-63. doi: 10.22037/afb.v3i1.10544
Champagne CP, Gardner NJ, Roy D. Challenges in the addition of probiotic cultures to foods. Crit Rev Food Sci Nutr. 2005; 45(1): 61-84. doi: 10.1080/10408690590900144
Prado FC, Parada JL, Pandey A, Soccol CR. Trends in nondairy probiotic beverages. Food Res Intl. 2008; 41(2): 111-123. doi: 10.1016/j.foodres.2007.10.010
Weinbreck F, Bodnár I, Marco M. Can encapsulation lengthen the shelf-life of probiotic bacteria in dry products?.Int J Food Microbiol. 2010; 136(3): 364-367. doi: 10.1016/j.ijfood-micro.2009.11.004
Foroutan NS, Tabandeh F, Khodabandeh M, Mojgani N, Maghsoudi A, Moradi M. Isolation and identification of an indigenous probiotic Lactobacillus Strain: Its encapsulation with natural branched polysaccharids to improve bacterial viability. Appl Food Biotechnol. 2017; 4(3): 133-142. doi: 10.22037/afb.v4i3.16471
Riaz QUA, Masud T. Recent Trends and applications of encapsulating materials for probiotic stability. Crit Rev Food Sci Nutr. 2013; 53(3): 231-244. doi: 10.1080/10408398.2010-.524953
Ying D, Schwander S, Weerakkody R, Sanguansri L, Gantenbein-Demarchi C, Augustin MA. Microencapsulated Lactobacillus rhamnosus GG in whey protein and resistant starch matrices: Probiotic survival in fruit juice. J Funct Foods. 2013; 5(1): 98-105. doi: 10.1016/j.jff.2012.08.009
Gandomi H, Abbaszadeh S, Misaghi A, Bokaie S, Noori N. Effect of chitosan-alginate encapsulation with inulin on survival of Lactobacillus rhamnosus GG during apple juice storage and under simulated gastrointestinal conditions. Food Sci Technol. 2016; 69: 365-371. doi: 10.1016/j.lwt.2016.-01.064
Rodrigues D, Sousa S, Gomes AM, Pintado MM, Silva JP, Costa P, et al. Storage stability of Lactobacillus paracasei as free cells or encapsulated in alginate-based microcapsules in low pH fruit juices. Food Bioprocess Tech. 2012; 5(7): 2748-2757. doi: 10.1007/s11947-011-0581-z
Coghetto CC, Flores SH, Brinques GB, Ayub MAZ. Viability and alternative uses of a dried powder, microencapsulated Lactobacillus plantarum without the use of cold chain or dairy products. Food Sci Technol. 2016; 71: 54-59. doi:10.10-16/j.lwt.2016.03.020
Doherty S, Auty M, Stanton C, Ross R, Fitzgerald G, Brodkorb A. Application of whey protein micro-bead coatings for enhanced strength and probiotic protection during fruit juice storage and gastric incubation. J Microencapsul. 2012; 29(8): 713-728. doi: 10.3109/02652048.2011.638994
Khorasani AC, Shojaosadati SA. Bacterial nanocellulosepectin bionanocomposites as prebiotics against drying and gastrointestinal condition. Int J Biol Macromolec. 2016; 83:9-18. doi: 10.1016/j.ijbiomac.2015.11.041
Bogusławska-Tryk M, Szymeczko R, Piotrowska A, Burlikowska K, Śliżewska K. Ileal and cecal microbial population and short-chain fatty acid profile in broiler chickens fed diets supplemented with lignocellulose. Pak Vet J . 2015;35(2): 212-216.
Okamoto Y, Nose M, Miyatake K, Sekine J, Oura R, Shigemasa Y, et al. Physical changes of chitin and chitosan in canine gastrointestinal tract. Carbohydr Polym. 2001; 44(3):211-215. doi: 10.1016/S0144-8617(00)00229-0
Khorasani AC, Shojaosadati SA. Pectin-non-starch nanofibers biocomposites as novel gastrointestinal-resistant prebiotics. Int J Biol Macromolec. 2017; 94, Part A: 131-144. doi: 10.10-16/j.ijbiomac.2016.10.011
May CD. Industrial pectins: Sources, production and applications. Carbohydr Polym. 1990; 12(1): 79-99. doi:10.1016/0144-8617(90)90105-2
Blaiotta G, La Gatta B, Di Capua M, Di Luccia A, Coppola R, Aponte M. Effect of chestnut extract and chestnut fiber on viability of potential probiotic Lactobacillus strains under gastrointestinal tract conditions. Food Microbiol. 2013; 36(2):161-169. doi: 10.1016/j.fm.2013.05.002
Huebner J, Wehling RL, Hutkins RW. Functional activity of commercial prebiotics. Int Dairy J. 2007; 17(7): 770-775. doi:10.1016/j.idairyj.2006.10.006
Faye T, Tamburello A, Vegarud G, Skeie S. Survival of lactic acid bacteria from fermented milks in an in vitro digestion model exploiting sequential incubation in human gastric and duodenum juice. J Dairy Sci. 2012; 95(2): 558-566. doi:10.3168/jds.2011-4705
Bedani R, Vieira ADS, Rossi EA, Saad SMI. Tropical fruit pulps decreased probiotic survival to in vitro gastrointestinal stress in synbiotic soy yoghurt with okara during storage. Food Sci Technol. 2014; 55(2): 436-443. doi:10.1016/j.lwt.20-13.10.015
Acosta-Estrada BA, Gutiérrez-Uribe JA, Serna-Saldívar SO. Bound phenolics in foods, a review. Food Chem. 2014; 152: 46-55. doi: 10.1016/j.foodchem.2013.11.093
Conde E, Gullón P, Moure A, Domínguez H, Parajó JC. Fractionation of industrial solids containing barley husks in aqueous media. Food Bio Prod Process. 2009; 87(3): 208-214. doi: 10.1016/j.fbp.2009.03.007
Narayanan D, Jayakumar R, Chennazhi K. Versatile carboxymethyl chitin and chitosan nanomaterials: a review. Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2014; 6(6):574-598. doi: 10.1002/wnan.1301
Gibson GR. Fiber and effects on probiotics (the prebiotic concept). Clin Nutr Suppl. 2004; 1(2): 25-31. doi: 10-.1016/j.clnu.2004.09.005
Ding W, Shah NP. Survival of free and microencapsulated probiotic bacteria in orange and apple juices. Int Food Res J.2008; 15(2): 219-232.
Pimentel TC, Madrona GS, Garcia S, Prudencio SH. Probiotic viability, physicochemical characteristics and acceptability during refrigerated storage of clarified apple juice suplemented with Lactobacillus paracasei ssp. paracasei and oligofructose in different package type. Food Sci Technol. 2015;63(1):415-422. doi:10.1016/j.lwt.2015.03.009
Davis C. Enumeration of probiotic strains: review of culturedependent and alternative techniques to quantify viable bacteria. J Microbiol Methods. 2014; 103: 9-17. doi: 10.10-16/j.mimet.2014.04.012
Leopold L, Horst D, Socaciu C. HPLC fingerprint of organic acids in fruit juices. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Agriculture. 2006;62: 288-292. doi: http: doi.org/10.15835/buasvmcnagr:1613
Nazzaro F, Fratianni F, Coppola R, Sada A, Orlando P. Fermentative ability of alginate-prebiotic encapsulated Lactobacillus acidophilus and survival under simulated gastrointestinal conditions. J Funct Foods. 2009; 1(3): 319-323. doi: 10.1016/j.jff.2009.02.001
Cruz AG, Sant’Ana AdS, Macchione MM, Teixeira ÂM, Schmidt FL. Milk drink using whey butter cheese (queijo manteiga) and acerola juice as a potential source of vitamin C. Food Bioprocess Tech . 2009;2(4): 368-373. doi: 10.100-7/s11947-008-0059-9
Shah N, Ding W, Fallourd M, Leyer G. Improving the stability of probiotic bacteria in model fruit juices using vitamins and antioxidants. J Food Sci. 2010;75(5): 278-282. doi: 10.11-11/j.1750-3841.2010.01628.x
Champagne CP, Gardner NJ. Effect of storage in a fruit drink on subsequent survival of probiotic lactobacilli to gastrointestinal stresses. Food Res Int. 2008; 41(5): 539-543. doi:10.1016/j.foodres.2008.03.003
Yu C-Y, Yin B-C, Zhang W, Cheng S-X, Zhang X-Z, Zhuo R-X. Composite microparticle drug delivery systems based on chitosan, alginate and pectin with improved pH-sensitive drug release property. Colloids Surf, B. 2009; 68(2): 245-249. doi: 10.1016/j.colsurfb.2008.10.013
Wu J, Meredith JC. Assembly of chitin nanofibers into porous biomimetic structures via freeze drying. ACS Macro Letters. 2014; 3(2): 185-190. doi: 10.1021/mz400543f
Ratanajiajaroen P, Ohshima M. Synthesis, release ability and bioactivity evaluation of chitin beads incorporated with curcumin for drug delivery applications. J Microencapsul. 2012; 29(6): 549-558. doi: 10.3109/02652048.2012.668954
Hiorth M, Tho I, Sande SA. The formation and permeability of drugs across free pectin and chitosan films prepared by a spraying method. Eur J Pharm Biopharm.. 2003; 56(2): 175-181. doi: 10.1016/S0939-6411(03)00065-1
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