Valorization of Pineapple Peels through Single Cell Protein Production Using Saccharomyces cerevisiae NCDC 364
Applied Food Biotechnology,
Vol. 6 No. 4 (2019),
24 September 2019
,
Page 255-263
https://doi.org/10.22037/afb.v6i4.25906
Abstract
Background and objective: Pineapple peels contain significant quantities of carbohydrates, which can be used as cheap raw materials for production of commercially important products through fermentation. The aim of this study was to use this feed stock for the cultivation of Saccharomyces cerevisiae NCDC 364 and its use as single cell protein.
Material and methods: The single cell protein was produced using discarded pineapple peels and Saccharomyces cerevisiae NCDC 364. Optimization of bioprocess variables (temperature, pH, incubation period, carbon source and nitrogen source) affecting single cell protein production was carried out using classical "one factor at a time" approach. The harvested cells from optimized media were screened for amino acid content using high-performance thin-layer chromatography.
Results and conclusion: The Saccharomyces cerevisiae NCDC 364 produced maximum single cell protein in pineapple peel based media, compared to non-optimized media. The "one factor at a time" approach showed that the maximum biomass production was achieved at optimized levels of temperature of 25ºC, pH of 5, incubation period of 120 h, carbon source of 1% sucrose and nitrogen source of 0.5% beef extract. The amino acid profiling of the harvested biomass using high-performance thin-layer chromatography analysis revealed that tryptophan included a comparatively higher concentration of 6.52%, followed by threonine (3.25%). Results of this study suggest that easily available raw materials such as fruit peels offer cost-effective substrates for production of commercially important microbial proteins for alarming global issues linked to protein malnutrition.
Conflict of interest: The authors declare no conflict of interest.
- ▪ Amino acid profiling ▪ Saccharomyces cerevisiae ▪ Single cell protein ▪ Pineapple peels
How to Cite
References
Chee JY, Lakshmanan M, Jeepery IF, Hairudin NHM, Sudesh K. The Potential Application of Cupriavidus necator as Polyhydroxyalkanoates Producer and Single Cell Protein: A Review on Scientific, Cultural and Religious Perspectives. Applied Food Biotechnology. 2019; 6(1): 19-34.
DOI: https://doi.org/10.22037/afb.v6i1.22234
Golaghaiee S, Ardestani F, Ghorbani HR. Microbial protein production from candida tropicalis ATCC13803 in a submerged batch fermentation process. Applied Food Biotechnology. 2017; 4(1): 35-42.
DOI: https://doi.org/10.22037/afb.v4i1.13698
Schaible UE, Kaufmann SH. Malnutrition and infection: complex mechanisms and global impacts. PLos Med. 2007; 4(5) : 40806-0812
DOI: 10.1371/journal.pmed.0040115
Ekubo AA, Abowei JFN. Review of Some Water Quality Management Principles Inculture Fisheries. Res J Appl Sci Eng Technol. 2011; 3(12): 1342-1357
Williams C. Kwashiorkor: A nutritional disease of children associated with a maize diet. Lancet. 1953; 226: 1151-1152
Amadou I, Kamara MT, Tidjani A, Foh MBK, Guo-Wei L. Physicochemical and nutritional analysis of fermented soyabean protein meal by Lactobacillus plantarum Lp6. World J Dairy and Food Sci. 2010; 5(2): 114-118
Kuijer OCH, Wielenga DK. A comparison of the environmental impact of meat and meat alternatives and the attractiveness of the alternatives to the consumers. Food and Agriculture Organization of the United Nations. http://agris.fao.org/agris-search/search.do?recordID=NL1999004941 (1999)
Asad MJ, Asghar M, Yaqub M, Shahzad K. Production of single cell protein from delignified corn cob Arachniotus species. Pak J Agr Sci. 2000; 37, 3-4
Parsons AB, Lopez A, Givoni IE, Williams DE, Gray CA, Porter J, Chua G, Sopko, R, Brost RL, Ho CH, Wang J, Ketela T, Brenner C, Brill JA, Fernandez G E, Lorenz TC, Payne GS, Ishihara S, Ohya Y, Andrews B, Hughes TR, Frey BJ, Graham TR, Andersen RJ, Boone C. Exploring the mode-of-action of bioactive compounds by chemical-genetic profiling in yeast. Cell. 2006; 126(3):611-625
DOI: 10.1016/j.cell.2006.06.040
Intriago P, Krauss E, Barniol R.The use of yeast and fungi as probiotics in Penaeus vannamei larviculture. Aquaculture. World Aquaculture Society, Baton Rouge. 263, (1998)
Sikka KC, Singh R, Gupta DP, Duggal SK. Comparative nutritive value of fish protein concentrate (FPC) from different species of fishes. J Agric Food Chem. 1979; 27(5) : 946-949
DOI: 10.1021/jf60225a032
Vasconcelos JMT, Rodrigues JM.L, Orvalho SCP, Alves SS, Mendes RL, Reis A. Effect of contaminants on mass transfer coefficients in bubble column and airlift contactors. Chem Eng Sci, 2003; 58(8):1431-1440
doi.org/10.1016/S0009-2509(02)00675-9
Rodriguez N, Garlick P. Introduction to Protein Summit 2007: Exploring the impact of high quality protein on optimal health. Am J Clin Nutr. 2008; 87(5): 1551S-1553S
DOI: 10.1093/ajcn/87.5.1551S
Gour Suman., Mathur Nupur., Singh Anuradha., Bhatnagar Pradeep. Single cell Protein: A Review. Int J Curr Microbiol App Sci. 2015; 4(9) : 251-262
Srividya AR, Vishnuvarthan VJ, Murugan M, Prajakt Gopal Dahake. Single Cell protein - A Review. Int. J. Pharm. Res. Scholars. 2013; 2: 2472-485
Kobatake M, Rij NJ, Placido MTLA, Uden, NV. Isolation of proteolytic psychrotrophic yeasts from fresh raw seafoods. Lett Appl Microbiol. 1992; 14(2) : 37-42
doi.org/10.1111/j.1472-765X.1992.tb00642.x
APHA, AWWA and WEF. 21st edition. Standard Methods for the Examination of Water and Wastewater, Washington, D.C (2005)
AOAC (Association of Official Analytical Chemists).: Official Methods of Analysis. 18th edition. AOAC edited by K. Helirich. Inc., Arlington, VA, USA (2005)
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193: 265-275
Hedge JE, Hofreiter B.T.In: Carbohydrate Chemistry 17 (Eds Whistler R.L. and Be Miller, J.N.), Academic Press New York (1962)
Miller GL. Use of dinitro salicylic acid reagent for determination of reducing sugar. Anal. Chem. 1959; 31: 426-427
Umesh M, Priyanka K, Thazeem B, Preethi K. Production of Single Cell Protein and Polyhydroxyalkanoate from Carica papaya Waste. Arab J Sci Eng. 42, 2361-2369 (2017)
doi: 10.1007/s13369-017-2519-x
Umesh M, Mani VM, Thazeem B, Preethi K. Statistical Optimization of Process Parameters for Bioplastic (PHA) Production by Bacillus subtilis NCDC0671 Using Orange Peel-Based Medium. Iran J Sci Technol Trans A Sci. 2017; 42(4) : 1947-1954
doi.org/10.1007/s40995-017-0457-9
Phaff HJ, Miller MW, Mrak EM.The Life of Yeasts. 2nd ed. Harvard University Press, London (1996)
Esabi Basaran Kurbanoglu, Omer Faruk Algur. Single cell protein production from ram horn hydrolysate by bacteria. Bioresour Technol. 2002; 85(2):125-129 doi.org/10.1016/S0960-8524(02)00094-9
Basheer Thazeem, Kathirvel Preethi, Mridul Umesh, Subramanian Radhakrishnan. Nutritive Characterization of Delimed Bovine Tannery Fleshings for their Possible Use as a Proteinaceous Aqua Feed Ingredient. Waste Biomass Valor. 2017; 9(8) : 1289-1301
doi: 10.1007/s12649-017-9922-0
Hemalatha R, Anbuselvi S. Physicochemical constituents of pineapple pulp and waste. J Chem Pharm Res. 2013; 5 (2) : 240-242
Huang YL, Chow CJ, Fang YJ. Preparation and physicochemical properties of fiber-rich fraction from pineapple peels as a potential ingredient. J Food Drug Anal. 2011; 19(3) : 318-323
Anupama, Ravindra. Value added food: single cell protein. Biotechnol Adv J Microbiol. 2000; 18(6) : 459-479
doi.org/10.1016/S0734-9750(00)00045-8
Gulab Sing, Anish Kumari, Aparna Mittal, Varsha Goel, AnithaYadav, Neeraj Kumar Aggarwal. Isolation and screening of polyhydroxyalkanoates producing bacteria from pulp, paper and cardboard industry waste water. Int J Biomater. 2013; 1:1-10
dx.doi.org/10.1155/2013/752821
Pranita Raju Uchakalwar, Anita M Chandak. Production of single cell protein from fruits waste by Saccharomyces cerevisiae. Int J Adv Biotech and Res. 2014; 1: 5770 – 776
Maragatham C, Panneerselvam A. Production of single cell protein from yeast using papaya extract medium. Adv Appl Sci Res. 2011; 2: 14 – 18
Chi ZM, Zhao SZ. Optimization of medium and cultivation condition for pullulan production by new pullulan-producing yeast strain. Enzyme Microb Technol. 2003; 33: 206 – 211.
doi.org/10.1016/S0141-0229(03)00119-4
Irfan M, Nazir MI, Nadeem M, Gulsher M, Syed Q, Baig S. Optimization of process parameters for the production of single cell biomass of Candida utilis in solid state fermentation. American-Eurasian Journal of Agricultural and Environmental Sciences. 2011; 10: 264-270.
Ardestani F, Alishahi F. Optimization of single cell protein production by Aspergillus niger using Taguchi approach. Journal of food biosciences and technology. 2015; 5(2): 73-79.
Ritchie F, Bain RA, McQuilken MP. Effects of nutrient status, temperature and pH on mycelial growth, sclerotial production and germination of Rhizoctonia solani from potato. J. Plant Pathol. 2009; 91(3) :589-596
Raimbault M, Alazard D. Cultural method to study fungal growth in solid fermentation. Eur J Appl Microbial Biotechnol. 1980; 9(3) :199-209
DOI: 10.1007/BF00504486
Khare SK, Jha Krishna, Gandhi AP. Glucomylase production by solid state fermentation from soypulp (okara). J Dairy Foods Home Sci. 1998; 17: 85-90
Mahmood Khan Yousufi. Impact of pH on the single cell protein produced on okara-wheat grit substrates using Rhizopus oligosporus and Aspergillus oryzae. IOSR J Environ Sci Toxicol Food Technol. 2012; 1: 32-35
DOI: 10.9790/2402-0123235
Hosam HH. Production of mini food by Aspergillus niger, Rhizopus oryzae and Saccharomyces cerevisiae using orange peel. Rom Biotechnol. Lett. 2013; 18:7924-7946
- Abstract Viewed: 1841 times
- PDF Downloaded: 1228 times