Research/Original Articles

Estimation of human absorbed dose of 99mTc-MAA using MIRD method based on animal data and comparison with MCNP simulation code

Masoumeh Naserpour, Saeed Mohammadi, Seyed Pezhman Shirmardi

Archives of Advances in Biosciences, Vol. 12 No. 1 (2021), 23 February 2021 , Page 1-6

Introduction: 99mTc-Macro Aggregated-Albumin (99mTc-MAA) has been evaluated as a useful perfusion study agent. In this study, the human absorbed dose of 99mTc-MAA was estimated with MIRD and MCNP methods based on animal biodistribution data and finally compared with ICRP publication data.

Materials and Methods: In this study, for investigating the biodistribution of 99mTc-MAA, after radiolabeling of MAA with Technetium-99m, it was injected to mice via the tail vein. After 1-120 min post injection, the mice were sacrificed and some of their tissues dissected and counted for calculating the percentage of the injected dose per gram (% ID/g) and the absorbed dose. Then, the obtained data was converted to equivalent data in human for each tissue.

Results: Dose prediction shows that the highest absorbed dose is observed in the lungs (MIRD: 6.8E-2 mGy/MBq, MCNP: 6.32E-2 mGy/MBq). There is good agreement between the results obtained from MIRD and MCNP simulation for lungs.

Conclusion: According to the present results and comparison with ICRP publication data, animal dissection model and simulation MCNP code can be useful tools for internally-absorbed dose estimation of pulmonary radiopharmaceuticals.

Evaluation of Protective and Immunomodulatory Effects of Hydroalcoholic Extract of Scrophularia striata on Silver Nanoparticle-Induced Toxicity in Male Rats

Masoud Shamohammadi, Mehrdad Pooyanmehr, Ali Maleki, Samad Alimohammadi

Archives of Advances in Biosciences, Vol. 12 No. 1 (2021), 23 February 2021 , Page 7-17

Introduction: Recently, silver nanoparticles (Ag-NPs) have found extensive and raising biomedical applications. Ag-NPs may lead to increased rate of toxicity on human health and environment. Because of the high antioxidant potential of the Scrophularia striata, the aim of the present study was to investigate the protective influence of Scrophularia striata against Ag-NPs-induced toxicity.

Materials and Methods: Thirty male Wistar rats were randomly divided into 5 groups (n=6 for each group). Group 1 was normal control rats. Group 2 received only Ag-NPs (200 ppm). In groups 3 to 5, the rats were pretreated with different concentrations (20, 60 and 180 mg/kg) of the Scrophularia striata extract, respectively and then were treated with Ag-NPs to induce toxicity. Animals were treated once daily by gavage over a period of 30 days. At the end of the treatment period, blood samples were collected and serum IgG, IgM, C3, C4, and CRP levels were determined. Data were statistically analyzed through one-way ANOVA, followed by Tukey’s post hoc test.

Results: Oral administration of Ag-NPs evoked a significant increase in the serum IgG, IgM, C3, C4, and CRP levels, compared with those in the control group (P<0.05). These changes were ameliorated through treatment with Scrophularia striata extract at different doses as compared with the Ag-NPs-treated group (P<0.05).

Conclusion: The extract was found to be as an effective immunomodulatory agent against Ag-NPs-induced toxicity presumably due to its active compounds with medicinal value.

Cellular Effects of Wound Fluid (Seroma) from Tumor Bed on Human Breast Cancer Cell Lines

Minoo Shahani, Fatemeh Rouhollah, Fereshteh Atabi, Shadi Hajrasouliha, Majid Samsami

Archives of Advances in Biosciences, Vol. 12 No. 1 (2021), 23 February 2021 , Page 18-24

Introduction: Post-lumpectomy wound fluid (seroma) contains many proteins from tumor bed due to physiologic answer to operation and wound healing process. Some cellular tests had been performed on different types of breast cancer (BC) cell lines and normal cell line while treated with seroma.

Materials and Methods: The wound fluid samples were collected from BC patients. The human BC cell lines included MCF-7, MDA-MB-231 as well as normal non-tumorigenic epithelial cell line (MCF-10).

Results: Seroma could inhibit various cancer cells proliferation pattern in comparison with the normal cell. Concerning the cell death, aggressive MDA-MB-231 cells were put into the apoptosis process. Besides, seroma could decrease colony count and size and changed the clone morphology from holoclone to paraclone. Regarding the invasion assay, seroma significantly inhibited cell motility.

Conclusion: By remaining in tumor bed, seroma can induce inhibitory pattern of proliferation, and change the morphology of cancer cell colony and cell motility, consequently leading to positive impact on patients who suffer from   cancer.