Investigating the Relationship between the Expression Level of Membrane-Bound Mucin (MUC12) and Clinicopathological Characterization of Colorectal Cancer
Archives of Advances in Biosciences,
Vol. 12 No. 1 (2021),
23 Esfand 2021
,
Page 31-36
https://doi.org/10.22037/aab.v12i1.34371
Abstract
Background: Colorectal cancer (CRC) is one of the most common cancers in the world and has a high mortality rate. It is accepted that dysfunction in the expression of mucins are associated with the occurrence and development of CRC. Therefore, the aim of the present study was to investigate the expression of MUC12 gene in colorectal cancer and their relationship with clinicopathological variables.
Materials and Methods: This research was prospective case-control study. Tumors from CRC patients were collected from the Taleghani Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran. RNA extraction and cDNA synthesis were performed using the corresponding kits. The gene primer was designed and RT-PCR was used to evaluate gene expression. The t-test and ANOVA were used to examine the differences between the different groups. Data analysis was performed using Prism8 software. Data analysis was performed using Prism8 software.
Results: The results of the present study showed that the expression of MUC12 (P=0.0012) gene in patients with colorectal cancer were significantly different from tumor margin samples. There were also associations between the expression of the studied gene and clinicopathological variables such as grade and stage of colorectal cancer tumor as well as the age of the patients. The area under the curves (AUC) for the MUC12 0.953 (95% CI 7565 to 0.9897, P=0.0003) was calculated by ROC analysis.
Conclusion: It can be stated that malignant transformation of colorectal cells is accompanied by changes in the expression of membrane-bound MUC gene (MUC12) in colorectal cancer, which has a biomarker value for the diagnosis of colorectal cancer.
- Muc genes, Colorectal Cancer, Gene expression
How to Cite
References
2. Casali P, Jost L, Reichardt P, Schlemmer M, Blay J-Y, Group EGW. Gastrointestinal stromal tumours: ESMO clinical recommendations for diagnosis, treatment and follow-up. Annals of Oncology. 2009;20(suppl_4):iv64-iv7.
3. Arvelo F, Sojo F,Cotte C. Biology of colorectal cancer. Ecancermedicalscience. 2015; 9.520.
4. Lech G, Słotwiński R, Słodkowski M, Krasnodębski IW. Colorectal cancer tumour markers and biomarkers: Recent therapeutic advances. World journal of gastroenterology. 2016;22(5):1745.
5. Sonzogni A, Bianchi F, Fabbri A, Cossa M, Rossi G, Cavazza A, et al. Pulmonary adenocarcinoma with mucin production modulates phenotype according to common genetic traits: a reappraisal of mucinous adenocarcinoma and colloid adenocarcinoma.The Journal of Pathology:Clinical Reserch.2017;3(2):139-151
6. Li C, Zuo D, Yin L, Lin Y, Li C, Liu T, et al. Prognostic Value of MUC2 Expression in Colorectal Cancer: A Systematic Review and Meta-Analysis. Gastroenterol Res Pract. 2018; 2018:6986870.
7. Shyu M-K, Lin M-C, Shih J-C, Lee C-N, Huang J, Liao C-H, et al. Mucin 15 is expressed in human placenta and suppresses invasion of trophoblast-like cells in vitro. Human reproduction.2007;22(10):2723-2732
8. Kufe D W. Mucins in cancer: function, prognosis and therapy. Nature Reviews Cancer. 2009; 9(12): 874-885.
9. Terry S, Savagner P, Ortiz-Cuaran S, Mahjoubi L, Saintigny P, Thiery J-P, et al. New insights into the role of EMT in tumor immune escape .Molecular Oncology .2017;11(7):824-846
10. McCOOL DJ, Forstner J, Forstner G. Regulated and unregulated pathways for MUC2 mucin secretion in human colonic LS180 adenocarcinoma cells are distinct. Biochemical Journal. 1995;312(1):125-33.
11. Betge J, Schneider NI, Harbaum L, Pollheimer MJ, Lindtner RA, Kornprat P, et al. MUC1, MUC2, MUC5AC, and MUC6 in colorectal cancer: expression profiles and clinical significance. Virchows Archiv. 2016;469(3):255-65.
12. Hollingsworth MA, Swanson BJ. Mucins in cancer: protection and control of the cell surface. Nat Rev Cancer 2004; 4: 45– 60.
13. Packer LM, Williams SJ, Callaghan S, Gotley DC, McGuckin MA. Expression of the cell surface mucin gene family in adenocarcinomas. Int J Oncol 2004; 25: 1119– 26.
14. Cavallaro U, Christofori G. Cell adhesion and signalling by cadherins and Ig‐CAMs in cancer. Nat Rev Cancer 2004; 4: 118– 32.
- Abstract Viewed: 97 times
- PDF Downloaded: 118 times