Archives of Advances in Biosciences

Abstract

     The aim of this study was to investigate the in vitro developmental competence of isolated pre-antral follicles derived from vitrified ovaries in the presence of coenzyme Q₁₀ (CoQ₁₀). Mice pre-antral follicles derived from fresh and vitrified-warmed ovarian tissues were in vitro cultured individually in α-MEM medium supplemented with or without CoQ₁₀, followed by adding human Chorionic Gonadotropin (hCG) to induce ovulation. The follicle development parameters and ovulated oocyte maturationwere assessed.The diameter and development of pre-antral follicles and oocyte maturation rates were significantly higher in CoQ₁₀ pretreatment groups of both vitrified and fresh samples compared to the respective CoQ₁₀free conditions groups. CoQ₁₀ improves the in vitrodevelopment of pre-antral follicles derived from fresh and vitrified –warmed ovaries.