Antimicrobial effects of Zingiber officinale extracts against multi-drug resistant Acinetobacter baumannii clinical isolates recovered from hospitalized patients in ICU
Archives of Advances in Biosciences,
Vol. 9 No. 3 (2018),
3 July 2018
,
Page 42-48
https://doi.org/10.22037/jps.v9i3.18624
Abstract
Zingiber officinale as an herbal medicinal plant is used for its potential antimicrobial activity against different microbial pathogens. Multi-drug resistant Acinetobacter baumannii as an important nosocomial pathogen especially in intensive care units is responsible for a wide range of serious infections in humans. The purpose of this study was to investigate the antimicrobial activity of Z. officinale extracts on growth of A. baumannii strains isolated from hospitalized patients in intensive care units in Tehran, Iran. During a 9 month study, 50 multi-drug resistant A. baumannii isolates were recovered from patients in ICU. The Kirby-Bauer disk diffusion method was used to determine resistance patterns of Multi-drug resistant A. baumannii isolates to antimicrobial agents. Micro-broth dilution method was used to determine the antimicrobial activity of methanol, acetone, and chloroform extracts of Z. officinale against multi-drug resistant A. baumannii isolates. The results of susceptibility testing showed that all the isolates were resistant to ceftriaxone, ciprofloxacin, ceftazidime, cefotaxime, cefepime and piperacillin. Resistance to colistin was found to be low (4%) and exhibited good antibacterial activity against tested isolates. This study’s findings revealed that methanol, acetone, and chloroform extracts of Z. officinale have anti-bacterial activity against tested bacterial isolates. Based on the results, the chloroform extracted fraction showed the highest level of activity at a minimal inhibitory concentration of 25 mg/ml on multi-drug resistant A. baumannii (64%). The minimal inhibitory concentration of ginger extract was as low as 3.2 mg/ml. The present study indicated that Z. officinale extracts, at various concentrations could be used as an antibacterial agent for treatment of patients in ICUs.
- Acinetobacter baumannii
- Ginger
- Antibacterial susceptibility
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References
Miller CS, Epstein JB, Hall EH, Sirois D. Changing oral care needs in the United States: the continuing need for oral medicine. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology. 2001;91(1):34-44.
Haas CN, Rose JB, Gerba CP. Quantitative microbial risk assessment: John Wiley & Sons; 1999.
Peleg AY, Seifert H, Paterson DL. Acinetobacter baumannii: emergence of a successful pathogen. Clinical microbiology reviews. 2008;21(3):538-82.
Dijkshoorn L, Nemec A, Seifert H. An increasing threat in hospitals: multidrug-resistant Acinetobacter baumannii. Nature Reviews Microbiology. 2007;5(12):939-51.
Livermore DM. Minimising antibiotic resistance. The Lancet infectious diseases. 2005;5(7):450-9.
Goudarzi M, Azimi H. Dissemination of Classes 1, 2, and 3 Integrons in Acinetobacter baumannii Strains Recovered from Intensive Care Units Using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism. Jundishapur Journal of Microbiology. 2017;10(5).
Sarma B, Acharya C, Joshi S. Pseudomonads: a versatile bacterial group exhibiting dual resistance to metals and antibiotics. African Journal of Microbiology Research. 2010;4(25):2828-35.
Wagner H, Ulrich-Merzenich G. Synergy research: approaching a new generation of phytopharmaceuticals. Phytomedicine. 2009;16(2):97-110.
Fabricant DS, Farnsworth NR. The value of plants used in traditional medicine for drug discovery. Environmental health perspectives. 2001;109(Suppl 1):69.
Verma VC, Gond SK, Kumar A, Mishra A, Kharwar RN, Gange AC. Endophytic actinomycetes from Azadirachta indica A. Juss.: isolation, diversity, and anti-microbial activity. Microbial ecology. 2009;57(4):749-56.
Gurib-Fakim A. Medicinal plants: traditions of yesterday and drugs of tomorrow. Molecular aspects of Medicine. 2006;27(1):1-93.
Kalantar H, Sabetkasaei M, Shahriari A, Hoseini MHM, Mansouri S, Kalantar M, et al. The Effect of Rapamycin on Oxidative Stress in MCF-7 and MDA MB-231 Human Breast Cancer Cell Lines. Jundishapur Journal of Natural Pharmaceutical Products. 2016;11(3).
Goudarzi M, Kalantar M, Kalantari H. Protective effect of hydroalcoholic extract of Lavandula officinalis L. On gentamicin induced nephrotoxicity in rats. Toxicology Letters. 2016(258):284-5.
Elgayyar M, Draughon F, Golden D, Mount J. Antimicrobial activity of essential oils from plants against selected pathogenic and saprophytic microorganisms. Journal of food protection. 2001;64(7):1019-24.
Weerakkody NS, Caffin N, Turner MS, Dykes GA. In vitro antimicrobial activity of less-utilized spice and herb extracts against selected food-borne bacteria. Food Control. 2010;21(10):1408-14.
Goudarzi H, Azad M, Seyedjavadi SS, Azimi H, Chirani AS, Omrani VF, et al. Characterization of integrons and associated gene cassettes in Acinetobacter baumannii strains isolated from intensive care unit in Tehran, Iran. Journal of Acute Disease. 2016;5(5):386-92.
Cowan MM. Plant products as antimicrobial agents. Clinical microbiology reviews. 1999;12(4):564-82.
Stoilova I, Krastanov A, Stoyanova A, Denev P, Gargova S. Antioxidant activity of a ginger extract (Zingiber officinale). Food chemistry. 2007;102(3):764-70.
Wigler I, Grotto I, Caspi D, Yaron M. The effects of Zintona EC (a ginger extract) on symptomatic gonarthritis. Osteoarthritis and cartilage. 2003;11(11):783-9.
Ghayur MN, Gilani AH, Afridi MB, Houghton PJ. Cardiovascular effects of ginger aqueous extract and its phenolic constituents are mediated through multiple pathways. Vascular pharmacology. 2005;43(4):234-41.
Maizura M, Aminah A, Wan Aida W. Total phenolic content and antioxidant activity of kesum (Polygonum minus), ginger (Zingiber officinale) and turmeric (Curcuma longa) extract. International Food Research Journal. 2011;18(2).
Willetts KE, Ekangaki A, Eden JA. Effect of a ginger extract on pregnancy‐induced nausea: A randomised controlled trial. Australian and New Zealand journal of obstetrics and gynaecology. 2003;43(2):139-44.
Habib SHM, Makpol S, Hamid NAA, Das S, Ngah WZW, Yusof YAM. Ginger extract (Zingiber officinale) has anti-cancer and anti-inflammatory effects on ethionine-induced hepatoma rats. Clinics. 2008;63(6):807-13.
Bliddal H, Rosetzsky A, Schlichting P, Weidner M, Andersen L, Ibfelt H-H, et al. A randomized, placebo-controlled, cross-over study of ginger extracts and ibuprofen in osteoarthritis. Osteoarthritis and Cartilage. 2000;8(1):9-12.
Mirsalehian A, Bahador A, Bigverdi R, Goli H. Prevalence of MBL-producing Pseudomonas aeruginosa isolated from burn patients. Tehran University of Medical Sciences. 2011;68(10).
Meradji S, Barguigua A, Zerouali K, Mazouz D, Chettibi H, Elmdaghri N, et al. Epidemiology of carbapenem non-susceptible Pseudomonas aeruginosa isolates in Eastern Algeria. Antimicrobial resistance and infection control. 2015;4(1):27.
Usha M, Ragini S, Naqvi S. Antibacterial activity of acetone and ethanol extracts of Cinnamon (Cinnamomum zeylanicum) and Ajowan (Trachyspermum ammi) on four food spoilage bacteria. Int Res J Biol Sci. 2012;1(4):7-11.
Gull I, Saeed M, Shaukat H, Aslam SM, Samra ZQ, Athar AM. Inhibitory effect of Allium sativum and Zingiber officinale extracts on clinically important drug resistant pathogenic bacteria. Annals of clinical microbiology and antimicrobials. 2012;11(1):8.
Karuppiah P, Rajaram S. Antibacterial effect of Allium sativum cloves and Zingiber officinale rhizomes against multiple-drug resistant clinical pathogens. Asian Pacific journal of tropical biomedicine. 2012;2(8):597-601.
Adeshina G, Jibo S, Agu V, Ehinmidu J. Antibacterial activity of fresh juices of Allium cepa and Zingiber officinale against multidrug resistant bacteria. International Journal of Pharma and Bio Sciences. 2011;2(2):289-94.
Hindi NKK, Al-Mahdi ZKA, Chabuck ZAG. Antibacterial activity of the aquatic extractof fresh, dry powder ginger, apple vinegar extract of fresh ginger and crud oil of ginger (Zingiber officinale) against different types of bacteria in hilla city, Iraq. Prostate. 2014;3:6.
Aghazadeh M, Bialvaei AZ, Aghazadeh M, Kabiri F, Saliani N, Yousefi M, et al. Survey of the antibiofilm and antimicrobial effects of Zingiber officinale (in vitro study). Jundishapur journal of microbiology. 2016;9(2).
Wang HM, Chen CY, Chen HA, Huang WC, Lin WR, Chen TC, et al. Zingiber officinale (ginger) compounds have tetracycline‐resistance modifying effects against clinical extensively drug‐resistant Acinetobacter baumannii. Phytotherapy research. 2010;24(12):1825-30.
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