Adeyemo, A. O., Aborode, A. T., Bello, M. A., Obianuju, A. F., Hasan, M. M., Kehinde, D. O., Hossain, M. S., Bardhan, M., Imisioluwa, J. O. and A. A. Akintola, (2022). "Malaria vaccine: the lasting solution to malaria burden in Africa." Annals of Medicine and Surgery, 79: 104031. DOI: https://doi.org/10.1016/j.amsu.2022.104031.

Angenon, G., Van Montagu, M. and A. Depicker, (1990). "Analysis of the stop codon context in plant nuclear genes." FEBS Letters, 271(1-2): 144-146. DOI: https://doi.org/10.1016/0014-5793(90)80392-V.

Antony, H. A. and S. C. Parija, (2016). "Antimalarial drug resistance: an overview." Tropical Parasitology, 6(1): 30-41. DOI: https://doi.org/10.4103/2229-5070.175081.

Araujo, J. S. C., de Souza, B. C., Costa Junior, D. B., Oliveira, L. d. M., Santana, I. B., Duarte, A. A., Lacerda, P. S., dos Santos Junior, M. C. and F. H. A. Leite, (2018). "Identification of new promising Plasmodium falciparum superoxide dismutase allosteric inhibitors through hierarchical pharmacophore-based virtual screening and molecular dynamics." Journal of Molecular Modeling, 24: 1-17. DOI: https://doi.org/10.1007/s00894-018-3746-0.

Baek, G., Saeed, M. and H.-K. Choi, (2021). "Duckweeds: their utilization, metabolites and cultivation." Applied Biological Chemistry, 64: 1-15. DOI: https://doi.org/10.1186/s13765-021-00644-z.

Bertran, K., Thomas, C., Guo, X., Bublot, M., Pritchard, N., Regan, J.T., Cox, K.M., Gasdaska, J.R., Dickey, L.F., Kapczynski, D.R. and D.E. Swayne, (2015). "Expression of H5 hemagglutinin vaccine antigen in common duckweed (Lemna minor) protects against H5N1 high pathogenicity avian influenza virus challenge in immunized chickens." Vaccine, 33(30): 3456-3462. DOI: https://doi.org/10.1016/j.vaccine.2015.05.076.

Birkett, A.J. (2016). "Status of vaccine research and development of vaccines for malaria." Vaccine, 34(26): 2915-2920. DOI: https://doi.org/10.1016/j.vaccine.2015.12.074.

Chu, Y., Yu, D., Li, Y., Huang, K., Shen, Y., Cong, L., Zhang, J. and M. Wang, (2024). "A 5′ UTR language model for decoding untranslated regions of mRNA and function predictions." Nature Machine Intelligence, 6(4): 449-460. DOI: https://doi.org/10.1038/s42256-024-00823-9.

Cid, R. and J. Bolívar, (2021). "Platforms for production of protein-based vaccines: from classical to next-generation strategies." Biomolecules, 11(8): 1072. DOI: https://doi.org/10.3390/biom11081072.

Cohen, J., Nussenzweig, V., Vekemans, J. and A. Leach, (2010). "From the circumsporozoite protein to the RTS, S/AS candidate vaccine." Human Vaccines, 6(1): 90-96. DOI: https://doi.org/10.4161/hv.6.1.9677.

Crompton, P.D., Moebius, J., Portugal, S., Waisberg, M., Hart, G., Garver, L.S., Miller, L.H., Barillas-Mury, C. and S.K. Pierce, (2014). "Malaria immunity in man and mosquito: insights into unsolved mysteries of a deadly infectious disease." Annual Review of Immunology, 32(1): 157-187. DOI: https://doi.org/10.1146/annurev-immunol-032713-120220.

Denecke, J., De Rycke, R. and J. Botterman, (1992). "Plant and mammalian sorting signals for protein retention in the endoplasmic reticulum contain a conserved epitope." The EMBO journal, 11(6): 2345-2355. DOI: https://doi.org/10.1002/j.1460-2075.1992.tb05294.x.

Dhanda, S.K., Vir, P. and G.P. Raghava, (2013). "Designing of interferon-gamma inducing MHC class-II binders." Biology Direct, 8: 1-15. DOI: https://doi.org/10.1186/1745-6150-8-30.

Duffy, P.E. and J.P. Gorres, (2020). "Malaria vaccines since 2000: progress, priorities, products." npj Vaccines, 5(1): 48. DOI: https://doi.org/10.1038/s41541-020-0196-3.

Duffy, P.E., Sahu, T., Akue, A., Milman, N. and C. Anderson, (2012). "Pre-erythrocytic malaria vaccines: identifying the targets." Expert Review of Vaccines, 11(10): 1261-1280. DOI: https://doi.org/10.1586/erv.12.92.

EL‐Manzalawy, Y., Dobbs, D. and V. Honavar, (2008). "Predicting linear B‐cell epitopes using string kernels." Journal of Molecular Recognition, 21(4): 243-255. DOI: https://doi.org/10.1002/jmr.893.

Esquenazi, J. (2023). Evaluation of the humoral immune responses to plasmodium vivax circumsporozoite protein (CSP)-based pre-erythrocytic vaccine candidates, Master's thesis, Global Communicable Disease, College of Public Health University of South Florida. https://digitalcommons.usf.edu/etd/10111.

Feng, B., Fang, Y., Xu, Z., Xiang, C., Zhou, C., Jiang, F., Wang, T. and H. Zhao, (2017). "Development of a new marker system for identification of Spirodela polyrhiza and Landoltia punctata." International Journal of Genomics, 2017(1): 5196763. DOI: https://doi.org/10.1155/2017/5196763.

Firsov, A., Tarasenko, I., Mitiouchkina, T., Ismailova, N., Shaloiko, L., Vainstein, A. and S. Dolgov, (2015). "High-yield expression of M2e peptide of avian influenza virus H5N1 in transgenic duckweed plants." Molecular Biotechnology, 57: 653-661. DOI: https://doi.org/10.1007/s12033-015-9855-4.

Garnier, J., J.-F. Gibrat and B. Robson (1996). "[32] GOR method for predicting protein secondary structure from amino acid sequence." In: Doolittle, R.F. (eds), Methods in Enzymology, Elsevier. V. 266, pp. 540-553. DOI: https://doi.org/10.1016/S0076-6879(96)66034-0.

Gasdaska, J.R., Spencer, D. and L. Dickey, (2003). "Advantages of therapeutic protein production in the aquatic plant Lemna." BioProcessing Journal, 2: 49-56. DOI: https://doi.org/10.12665/J22.Gasdaska.

Gasteiger, E., Hoogland, C., Gattiker, A., Duvaud, S.E., Wilkins, M.R., Appel, R.D. and A. Bairoch, (2005). "Protein identification and analysis tools on the ExPASy server." In: Walker, J.M. (eds), The proteomics protocols handbook, Protocols Handbooks. Humana Press, pp.571-607.. DOI: https://doi.org/10.1385/1-59259-890-0:571.

Gimenez, A.M., Lima, L.C., Françoso, K.S., Denapoli, P.M., Panatieri, R., Bargieri, D.Y., Thiberge, J.M., Andolina, C., Nosten, F., Renia, L. and R.S. Nussenzweig, (2017). "Vaccine containing the three allelic variants of the Plasmodium vivax circumsporozoite antigen induces protection in mice after challenge with a transgenic rodent malaria parasite." Frontiers in Immunology, 8: 1275. DOI: https://doi.org/10.3389/fimmu.2017.01275.

Heenatigala, P. and H. Hou, (2024). "Duckweed, an Efficient Green Bio-Factory for the Production of Recombinant Proteins." In: Kole, C., Chaurasia, A., Hefferon, K.L., Panigrahi, J. (eds), Applications of Plant Molecular Farming. Concepts and Strategies in Plant Sciences. pp.613-630. Springer, Singapore. DOI: https://doi.org/10.1007/978-981-97-0176-6_22.

Huang, F., Feng, X.Y., Zhou, S.S., Tang, L.H. and Z.G. Xia, (2022). "Establishing and applying an adaptive strategy and approach to eliminating malaria: practice and lessons learnt from China from 2011 to 2020." Emerging microbes & infections, 11(1): 314-325. DOI: https://doi.org/10.1080/22221751.2022.2026740.

Ikai, A. (1980). "Thermostability and aliphatic index of globular proteins." The Journal of Biochemistry, 88(6): 1895-1898. DOI: https://doi.org/10.1093/oxfordjournals.jbchem.a133168.

Ippolito, M.M., Moser, K.A., Kabuya, J.B.B., Cunningham, C. and J.J. Juliano, (2021). "Antimalarial drug resistance and implications for the WHO global technical strategy." Current Epidemiology Reports, 8: 46-62. DOI: https://doi.org/10.1007/s40471-021-00266-5.

Jaiswal, M., Zahra, S. and S. Kumar. (2020). "Bioinformatics tools for epitope prediction." In: Singh, S. (eds), Systems and Synthetic Immunology . Springer, Singapore. pp. 103-124. DOI: https://doi.org/10.1007/978-981-15-3350-1_4.

Jazayeri, S.D., Lim, H.X., Shameli, K., Yeap, S.K. and C.L. Poh, (2021). "Nano and microparticles as potential oral vaccine carriers and adjuvants against infectious diseases." Frontiers in pharmacology, 12: 682286. DOI: https://doi.org/10.3389/fphar.2021.682286.

Kalita, M.P., Basumatary, T.K., Medhi, S. and R.H. Begum, (2023). "Toll-like receptor 2-mediated downstream cytokine levels as determinant of malaria pathogenesis." Indian Journal of Medical Research, 157(4): 304-310. DOI: https://doi.org/10.4103/ijmr.IJMR_1180_19.

Kang, J.Y., Nan, X., Jin, M.S., Youn, S.J., Ryu, Y.H., Mah, S., Han, S.H., Lee, H., Paik, S.G. and J.O. Lee, (2009). "Recognition of lipopeptide patterns by Toll-like receptor 2-Toll-like receptor 6 heterodimer." Immunity, 31(6): 873-884. DOI: https://doi.org/10.1016/j.immuni.2009.09.018.

Khvatkov, P., Firsov, A., Mitiouchkina, T., Chernobrovkina, M. and S. Dolgov, (2021). "Duckweeds for the production of therapeutic proteins." In: Malik, S. (eds), Exploring plant cells for the production of compounds of interest, Springer, Cham. pp. 125-144. DOI: https://doi.org/10.1007/978-3-030-58271-5_5.

Kim, L., Martinez, C.J., Hodgson, K.A., Trager, G.R., Brandl, J.R., Sandefer, E.P., Doll, W.J., Liebowitz, D. and S.N. Tucker, (2016). "Systemic and mucosal immune responses following oral adenoviral delivery of influenza vaccine to the human intestine by radio controlled capsule." Scientific Reports, 6(1): 37295. DOI: https://doi.org/10.1038/srep37295.

Ko, S.M., Sun, H.J., Oh, M.J., Song, I.J., Kim, M.J., Sin, H.S., Goh, C.H., Kim, Y.W., Lim, P.O., Lee, H.Y. and S.W. Kim, (2011). "Expression of the protective antigen for PEDV in transgenic duckweed, Lemna minor." Horticulture, Environment, and Biotechnology, 52 (2011): 511-515. DOI: https://doi.org/10.1007/s13580-011-0007-x.

Kogan, F. (2020). "Malaria burden." In: Kogan, F. (eds), Remote Sensing for Malaria: Monitoring and Predicting Malaria from Operational Satellites. Springer Remote Sensing/Photogrammetry. Springer, Cham. pp. 15-41. DOI: https://doi.org/10.1007/978-3-030-46020-4_2.

Kozak, M. (1989). "The scanning model for translation: an update." The Journal of cell biology 108(2): 229-241. DOI: https://doi.org/10.1083/jcb.108.2.229.

Kozlov, O.N., Mitiouchkina, T.Y., Tarasenko, I.V., Shaloiko, L.A., Firsov, A.P. and S.V. Dolgov, (2019). "Agrobacterium-mediated transformation of Lemna minor L. with Hirudin and β-glucuronidase genes." Applied Biochemistry and Microbiology, 55: 805-815. DOI: https://doi.org/10.1134/S0003683819080076.

Kulshreshtha, A., Sharma, S., Padilla, C.S. and K.K. Mandadi, (2022). "Plant-based expression platforms to produce high-value metabolites and proteins." Frontiers in Plant Science, 13: 1043478. DOI: https://doi.org/10.3389/fpls.2022.1043478.

Kupani, M., Pandey, R.K., Vashisht, S., Singh, S., Prajapati, V.K. and S. Mehrotra, (2023). "Prediction of an immunogenic peptide ensemble and multi-subunit vaccine for Visceral leishmaniasis using bioinformatics approaches." Heliyon, 9(12): e22121. DOI: https://doi.org/10.1016/j.heliyon.2023.e22121.

Kwong, K.W.Y., Xin, Y., Lai, N.C.Y., Sung, J.C.C., Wu, K.C., Hamied, Y.K., Sze, E.T.P. and D.M.K. Lam, (2023). "Oral vaccines: A better future of immunization." Vaccines, 11(7): 1232. DOI: https://doi.org/10.3390/vaccines11071232.

Kyte, J. and R. F. Doolittle, (1982). "A simple method for displaying the hydropathic character of a protein." Journal of Molecular Biology, 157(1): 105-132. DOI: https://doi.org/10.1016/0022-2836(82)90515-0.

Laskowski, R.A., MacArthur, M.W., Moss, D.S. and J.M. Thornton, (1993). "PROCHECK: a program to check the stereochemical quality of protein structures." Journal of Applied Crystallography, 26(2): 283-291. DOI: https://doi.org/10.1107/S0021889892009944.

Li, T.T., Ogino, S. and Z.R. Qian, (2014). "Toll-like receptor signaling in colorectal cancer: carcinogenesis to cancer therapy." World Journal of Gastroenterology, 20(47): 17699. DOI: https://doi.org/10.3748/wjg.v20.i47.17699.

Lill, M. A. and M. L. Danielson, (2011). "Computer-aided drug design platform using PyMOL." Journal of Computer-Aided Molecular Design, 25: 13-19. DOI: https://doi.org/10.1007/s10822-010-9395-8.

Lovell, S.C., Davis, I.W., Arendall III, W.B., De Bakker, P.I., Word, J.M., Prisant, M.G., Richardson, J.S. and D.C. Richardson, (2003). "Structure validation by Cα geometry: ϕ, ψ and Cβ deviation." Proteins: Structure, Function, and Bioinformatics, 50(3): 437-450. DOI: https://doi.org/10.1002/prot.10286.

Midekisa, A., Beyene, B., Mihretie, A., Bayabil, E. and M.C. Wimberly, (2015). "Seasonal associations of climatic drivers and malaria in the highlands of Ethiopia." Parasites & Vectors, 8: 1-11. DOI: https://doi.org/10.1186/s13071-015-0954-7.

Milner, D.A. (2018). "Malaria pathogenesis." Cold Spring Harbor Perspectives in Medicine, 8(1): a025569. DOI: https://doi.org/10.1101/cshperspect.a025569.

Moorthy, V.S., Newman, R.D. and J.M. Okwo-Bele, (2013). "Malaria vaccine technology roadmap." The Lancet, 382(9906): 1700-1701. DOI: http://dx.doi.org/10.1016/S0140-6736(13)62238-2.

Oli, A.N., Obialor, W.O., Ifeanyichukwu, M.O., Odimegwu, D.C., Okoyeh, J.N., Emechebe, G.O., Adejumo, S.A. and G.C. Ibeanu, (2020). "Immunoinformatics and vaccine development: an overview." ImmunoTargets and Therapy, 9: 13-30. DOI: https://doi.org/10.2147/ITT.S241064.

Pritam, M., Singh, G., Swaroop, S., Singh, A.K., Pandey, B. and S.P. Singh, (2020). "A cutting-edge immunoinformatics approach for design of multi-epitope oral vaccine against dreadful human malaria." International Journal of Biological Macromolecules, 158: 159-179. DOI: https://doi.org/10.1016/j.ijbiomac.2020.04.191.

Richards, J. S. and J. G. Beeson, (2009). "The future for blood‐stage vaccines against malaria." Immunology and Cell Biology, 87(5): 377-390. DOI: https://doi.org/10.1038/icb.2009.27.

Sagara, I., Healy, S.A., Assadou, M.H., Gabriel, E.E., Kone, M., Sissoko, K., Tembine, I., Guindo, M.A., Doucoure, M.B., Niaré, K. and A. Dolo, (2018). "Safety and immunogenicity of Pfs25H-EPA/Alhydrogel, a transmission-blocking vaccine against Plasmodium falciparum: a randomised, double-blind, comparator-controlled, dose-escalation study in healthy Malian adults." The Lancet Infectious Diseases, 18(9): 969-982. DOI: https://doi.org/10.1016/S1473-3099(18)30344-X.

Sanami, S., Rafieian-Kopaei, M., Dehkordi, K.A., Pazoki-Toroudi, H., Azadegan-Dehkordi, F., Mobini, G.R., Alizadeh, M., Nezhad, M.S., Ghasemi-Dehnoo, M. and N. Bagheri, (2022). "In silico design of a multi-epitope vaccine against HPV16/18." BMC Bioinformatics, 23(1): 311. DOI: https://doi.org/10.1186/s12859-022-04784-x.

Shabani, S.H., Zakeri, S., Salmanian, A.H., Amani, J., Mehrizi, A.A., Snounou, G., Nosten, F., Andolina, C., Mourtazavi, Y. and N.D. Djadid, (2017). "Biological, immunological and functional properties of two novel multi-variant chimeric recombinant proteins of CSP antigens for vaccine development against Plasmodium vivax infection." Molecular Immunology, 90: 158-171. DOI: https://doi.org/10.1016/j.molimm.2017.06.033Get rights and content.

Shang, T., Fang, C.M., Ong, C.E. and Y. Pan, (2023). "Heterologous expression of recombinant human cytochrome P450 (CYP) in Escherichia coli: N-terminal modification, expression, isolation, purification, and reconstitution." BioTech, 12(1): 17. DOI: https://doi.org/10.3390/biotech12010017.

Shanmugaraj, B., I. Bulaon, C.J. and W. Phoolcharoen, (2020). "Plant molecular farming: A viable platform for recombinant biopharmaceutical production." Plants, 9(7): 842. DOI: https://doi.org/10.3390/plants9070842.

Shanmugaraj, B., Loganathan, N. and H.M. Chandra, (2024). "Plant system as a versatile and robust platform for the development of vaccines against arboviral infections." Vacunas, 25(4): 492-501. DOI: https://doi.org/10.1016/j.vacune.2024.10.010.

Skwarczynski, M., Chandrudu, S., Rigau-Planella, B., Islam, M.T., Cheong, Y.S., Liu, G., Wang, X., Toth, I. and W.M. Hussein, (2020). "Progress in the development of subunit vaccines against malaria." Vaccines, 8(3): 373. DOI: https://doi.org/10.3390/vaccines8030373.

Smith, K.E., Zhou, M., Flis, P., Jones, D.H., Bishopp, A. and L. Yant, (2024). "The evolution of the duckweed ionome mirrors losses in structural complexity." Annals of Botany, 133(7): 997-1006. DOI: https://doi.org/10.1093/aob/mcae012.

Stanisic, D.I. and M.F. Good, (2023). "Malaria vaccines: progress to date." BioDrugs, 37(6): 737-756. DOI: https://doi.org/10.1007/s40259-023-00623-4.

Sun, Y., Cheng, J.J., Himmel, M.E., Skory, C.D., Adney, W.S., Thomas, S.R., Tisserat, B., Nishimura, Y. and Y.T. Yamamoto, (2007). "Expression and characterization of Acidothermus cellulolyticus E1 endoglucanase in transgenic duckweed Lemna minor 8627." Bioresource Technology, 98(15): 2866-2872. DOI: https://doi.org/10.1016/j.biortech.2006.09.055.

Thingujam, D., Pajerowska-Mukhtar, K.M. and M.S. Mukhtar, (2024). "Duckweed: Beyond an Efficient Plant Model System." Biomolecules, 14(6): 628. DOI: https://doi.org/10.3390/biom14060628.

Tripp, R.A. (2021). "Advances in vaccine development." Vaccines, 9(9): 1036. DOI: https://doi.org/10.3390/vaccines9091036.

Tsoumani, M.E., Voyiatzaki, C. and A. Efstathiou, (2023). "Malaria vaccines: from the past towards the mRNA vaccine era." Vaccines, 11(9): 1452. DOI: https://doi.org/10.3390/vaccines11091452.

Tusé, D., Tu, T. and K.A. McDonald, (2014). "Manufacturing economics of plant‐made biologics: case studies in therapeutic and industrial enzymes." BioMed Research International, 2014(1): 256135. DOI: https://doi.org/10.1155/2014/256135.

van Bergenhenegouwen, J., Plantinga, T.S., Joosten, L.A., Netea, M.G., Folkerts, G., Kraneveld, A.D., Garssen, J. and A.P. Vos, (2013). "TLR2 & Co: a critical analysis of the complex interactions between TLR2 and coreceptors." Journal of Leukocyte Biology, 94(5): 885-902. DOI: https://doi.org/10.1189/jlb.0113003.

Vu, A., Calzadilla, A., Gidfar, S., Calderon-Candelario, R. and M. Mirsaeidi, (2017). "Toll-like receptors in mycobacterial infection." European Journal of Pharmacology, 808: 1-7. DOI: https://doi.org/10.1016/j.ejphar.2016.10.018.

Walker, I.S. and S.J. Rogerson, (2023). "Pathogenicity and virulence of malaria: Sticky problems and tricky solutions." Virulence, 14(1): 2150456. DOI: https://doi.org/10.1080/21505594.2022.2150456.

Wang, P., Sidney, J., Kim, Y., Sette, A., Lund, O., Nielsen, M. and B. Peters, (2010). "Peptide binding predictions for HLA DR, DP and DQ molecules." BMC Bioinformatics, 11: 568. DOI: https://doi.org/10.1186/1471-2105-11-568.

Wiederstein, M. and M.J. Sippl, (2007). "ProSA-web: interactive web service for the recognition of errors in three-dimensional structures of proteins." Nucleic Acids Research, 35(suppl_2): W407-W410. DOI: https://doi.org/10.1093/nar/gkm290.

World Health Organization (2022). "Global report on infection prevention and control." Geneva, World Health Organization. Retrieved from: https://iris.who.int/handle/10665/354489.

Yadava, A., Hall, C.E., Sullivan, J.S., Nace, D., Williams, T., Collins, W.E., Ockenhouse, C.F. and J.W. Barnwell, (2014). "Protective efficacy of a Plasmodium vivax circumsporozoite protein-based vaccine in Aotus nancymaae is associated with antibodies to the repeat region." PLoS Neglected Tropical Diseases, 8(10): e3268. DOI: https://doi.org/10.1371/journal.pntd.0003268.

Yang, G.L., Feng, D., Liu, Y.T., Lv, S.M., Zheng, M.M. and A.J. Tan, (2021). "Research progress of a potential bioreactor: duckweed." Biomolecules, 11(1): 93. DOI: https://doi.org/10.3390/biom11010093.

Yang, J., Yan, R., Roy, A., Xu, D., Poisson, J. and Y. Zhang, (2015). "The I-TASSER Suite: protein structure and function prediction." Nature Methods, 12(1): 7-8. DOI: https://doi.org/10.1038/nmeth.3213.

Yang, J. and Y. Zhang, (2015). "I-TASSER server: new development for protein structure and function predictions." Nucleic Acids Research, 43(W1): W174-W181. DOI: https://doi.org/10.1093/nar/gkv342.

Zhang, Y. (2008). "I-TASSER server for protein 3D structure prediction." BMC bioinformatics 9: 1-8. DOI: https://doi.org/10.1186/1471-2105-9-40.