The Trends in Peptide and Protein Sciences (Trends Pept. Protein Sci.) is a peer-reviewed, online-only (previously print-online), scientific journal owned by Protein Technology Research Center, Shahid Beheshti University of Medical Sciences and documents in all important aspects of the research in peptides and proteins focusing on analytics and impurities, bioinformatics, biopharmaceuticals and vaccines, biotechnology, chemical synthesis, conformational analysis, design and  development of protein therapeutics, determination of structure, enzymology, folding and sequencing,  formulation and stability, function, genetics,  immunology, kinetics, modeling, molecular biology, pharmacokinetics and pharmacodynamics of therapeutic proteins and antibodies, pharmacology,  protein engineering and development, protein-protein interaction, proteomics, purification/expression/production, simulation, thermodynamics and  hydrodynamics and protein biomarkers. The aim of this Journal is to publish high quality original research articles, reviews, short communications and letters and to provide a medium for scientists and researchers to share their findings from the area of peptides and proteins. The Trends in Peptide and Protein Sciences is published in collaboration with Iranian Association of Pharmaceutical Scientists.

The Trends in Peptide and Protein Sciences has been granted the Scientific-Research Rank  by the Commission of Medical Sciences Journals of Ministry of Health, Treatment and Medical Education of I.R. Iran.

 

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Journal Info

Publisher:

Protein Technology Research Center, Shahid Beheshti University of Medical Sciences

Journal Name:

Trends in Peptide and Protein Sciences (TPPS)

Journal Abbreviation:

Trends Pept. Protein Sci.

eISSN:

2538-2446

Chairperson:

Reza Aboofazeli; PhD

Editor-in-Chief:

Bahram Kazemi; PhD

Managing Editor:

Maryam Tabarzad; PhD

Email:

TEL:

File:WhatsApp.svg - Wikipedia WhatsApp:

tipps@sbmu.ac.ir

+98 21 88648124 (8 a. m. to 4 p. m. Tehran, GMT+3.30)

+98 9380414297

linkedin.com/in/journal-trends-in-peptide-and-protein-sciences-314817216

Call for Papers: Electronic Volume 7 (2022)

We are pleased to invite your manuscript submissions to the electronic volume 7 (2022) of Trends in Peptide and Protein Sciences (TPPS). Based on the new TPPS publication policies, articles will be continuously published online only from 2018 ....

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Indexing in Indexcopernicus (ICI Journals Master List)

We would like to inform that the journal „Trends in Peptide and Protein Sciences (ISSN: 2538-2535)” has passed the evaluation process positively and is indexed in the ICI Journals Master List database for 2019 . Based on the information submitted in the evaluation and the analysis of the issues of the journal from 2019, Index Copernicus Experts calculated TPPS Index Copernicus Value (ICV) for 2019. ICV 2019 = 74.91

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Expression of the Mouse HSP27 Chaperone in CHO-K1 Cells for the Enhancement of Viable Cell Density in Batch Culture

Mohammad Reza Amini, Azam Rahimpour, Reyhaneh Hoseinpoor, Masoumeh Rajabibazl

Trends in Peptide and Protein Sciences, Vol. 7 (2022), , Page 1-5 (e2)

Chinese hamster ovary (CHO) cells are extremely vulnerable to cell viability loss in culture despite the availability of different nutrients supplementation strategies. As a result, extending the culture lifetime can profoundly increase recombinant protein expression. Overexpression of HSP27 and its anti-apoptotic effects have been shown in human cell lines in previous studies. In the current study, mouse HSP27 (mHSP27) was cloned in pcDNA 3.1 hygro expression vector and was expressed in CHO-K1 cells to assess its impacts on cell viability and growth. Expression of mHSP27 in CHO-K1 cells was confirmed using RT-PCR. A 3-fold enhancement in peak viable cell density of mHSP27 transfected clones was observed, and culture viability loss was delayed by 2 days compared to un-transfected cells. In future studies, the resulting mHSP27 CHO-K1 cells could be employed as a novel host system for the transient and stable expression of therapeutic recombinant proteins.


HIGHLIGHTS


  • Cell engineering is an effective strategy to cope with apoptosis in CHO cells.

  • HSP27 is involved in mammalian cell apoptosis.

  • Expression of the mouse HSP27 increased the viability and cell density of CHO-K1 cells.

Preparation and In Vitro Characterization of Crocin-loaded Casein Hydrogels

Fatemeh Mehryab, Fatemeh Taghizadeh, Azadeh Haeri

Trends in Peptide and Protein Sciences, Vol. 7 (2022), , Page 1-9 (e3)

Crocin, the main active constituent of saffron, has many important biological activities. Due to its anti-inflammatory properties, crocin can be potentially effective in different pathological conditions including oral ulcers. Novel drug delivery systems such as hydrogels have been used to increase the stability of crocin and provide a controlled release of this compound. Casein is the main protein of milk that possesses suitable properties for the fabrication of hydrogels. In this paper, casein-based hydrogels with different casein to crocin weight ratios were synthesized using the acid-gelation method. The prepared crocin-loaded hydrogels were characterized regarding their rheological behavior, drug content, swelling ratio, surface morphology, thermal stability, and in vitro release profile. The structure of casein hydrogels was characterized using Fourier transform infrared and X-ray diffraction. All formulations exhibited a pseudoplastic rheological behavior and there was no statistically significant difference in viscosity among them. Hydrogel with casein to crocin weight ratio of 10:1 had larger pores and demonstrated a higher swelling percentage and suitable thermal stability. All casein-based hydrogels demonstrated a slow release of crocin over 24 hours and the hydrogel with lower casein to crocin weight ratio had an increased release rate. Taken together, casein-based hydrogels were found to be effective carriers to provide a controlled release system for crocin delivery.


HIGHLIGHTS


  • Casein-based hydrogels were developed for delivery of crocin.

  • Casein-based hydrogels provided a controlled in vitro release profile for crocin.

  • Hydrogel with a lower casein ratio exhibited a higher release rate of crocin.

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