Purification and Properties of Thermostable Fucoidanase Produced by Recently Isolated Terrestrial Aspergillus flavus FS018 Characteristics of fucoidanase extracted from Aspergillus flavus FS018
Trends in Peptide and Protein Sciences,
Vol. 5 (2020),
4 March 2020
In this study fucoidanase produced by terrestrial Apsergillus flavus FS018 was purified and characterized. The pure fucoidanase enzyme was found to have an optimum activity of 20.8U/mL at 55 ºC and optimum activity of 17.2U/mL at pH 5.0. Furthermore, the fucoidanase retained 96% of its activity after 8 hours of incubation at 55 ºC. Metal ions such Mg2+ and Ca2+ ions were found to slightly enhance the activity of this enzyme while Na+, K+ had inhibitory effect on the activity. The enzyme was found to be active towards fucoidan consisting of α-1→4 and α-1→3 glycoside bonds in the main chains and also galactofucans group. Estimation of the kinetic parameters of the enzyme revealed that Km and Vmax to be 1.9 mM and 0.38 mg/min, respectively when fucoidan from Sargassum vulgare was used as substrate. SDS-PAGE analysis of the purified enzyme revealed that it’s a monomeric enzyme molecule with an estimated molecular weight of 70 kDa.
- Fucoidanase from Aspergillus flavus FS018 was purified and characterized.
- Molecular weight of the enzyme was estimated to be 70kDa.
- Enzyme was active towards fucoidan consisting of α-1→4 and α-1→3 glycoside bonds in the main chains and also galactofucans group.
- Aspergillus flavus
How to Cite
Ahmed, S., Riaz, S., Jamil, A., (2009). ″Molecular cloning of fungal xylanases: An overview.″ Applied Microbiology and Biotechnology, 84: 19–35.
Berka, R.M., Grigoriev, I.V., Otillar, R., Salamov, A., Grimwood, J., Reid, I., Ishmael, N., John, T., Darmond, C., Moisan, M.C. and B. Henrissat, (2011). ″Comparative genomic analysis of the thermophilic biomass-degrading fungi, Myceliophthora thermophila and Thielavia terrestris.″ Nature Biotechnology, 29; 922–927.
Chang, Y., Xue, C., Tang, Q., Li, D., Wu, X. and J. Wang, (2010). ″Isolation and characterization of a sea cucumber fucoidan utilizing marine bacterium.″ Letters in Applied Microbiology, 50: 301-307.
Cho, M.L., Lee, B.Y. and S.G. You, (2011). ″Relationship between oversulfation and conformation of low and high molecular weight fucoidans and evaluation of their in-vitro anticancer activity.″ Molecules, 16: 291-297.
Garuba, E.O. and A.A. Onilude, (2018). ″Immobilization of thermostable exo-inulinase from mutant thermophilic Aspergillus tamarii -U4 using kaolin clay and its application in inulin hydrolysis.″ Journal of Genetic Engineering and Biotechnology, 16(2): 341-346
Gomaa, M., Fawzy, M.A., Hifney, A.F. and K.M. Abdel-Gawad, (2018). ″Optimization of enzymatic saccharification of fucoidan and alginate from brown seaweed using fucoidanase and alginate lyase from the marine fungus Dendryphiella arenaria.″ Journal of Applied Phycology, 31:1955–1965.
Gurpilhares, D.B., Moreira, T.R, da Luz Bueno J., Cinelli, L.P., Mazzola, P.G., Pessoa, A. and L.D. Sette, (2016). ″Algae’s sulfated polysaccharides modifications: potential use of microbial enzymes.″ Process Biochemistry, 51, 989–998.
Khanh H.H.N., Dieu, V.T. Thinh, T.P.D. and P.T. San, (2019). ″Catalytic conditions of fucoidanase from Vasticardium flavum.″ Vietnam Journal of Science and Technology, 57 (1): 28-37
Kim, W.J., Park, J.W., Park, J.K, Choi, D.J. and Y.I. Park, (2015). ″Purification and characterization of a fucoidanase (FNase S) from a marine bacterium Sphingomonas paucimobilis PF-1.″ Marine Drugs, 13: 4398-4417.
Kusaykin, M.I., Silchenko, A.S., Zakharenko, A.M. and T.N. Zvyagintseva, (2016). ″Fucoidanases.″ Glycobiology, 26: 3–12.
Li, B., Lu, F., Wei, X. and R. Zhao, (2008). ″Fucoidan: structure and Bioactivity.″ Molecules, 13: 1671-1695
Li, B., Rui, X.Z. and J.W. Xin, (2008). ″Anticoagulant activity of fucoidan from Hizikia fusiforme.″ Agro Food Industry Hi-Tech, 19: 22-24.
Manivasagan P. and J. Oh, (2015). ″Production of a Novel Fucoidanase for the Green Synthesis of Gold Nanoparticles by Streptomyces sp. and Its Cytotoxic Effect on HeLa Cells.″ Marine Drugs 13: 6818-6837.
Miller, G.L. (1959). ″Use of dinitrosalicylic acid reagent for determination of reducing sugar.″ Analytical Chemistry, 31: 426-428.
Mourao, .PA.S. (2004). ″Use of sulfated fucans as anticoagulant and antithrombotic agents: future perspectives.″ Current Pharmaceutical Design, 10: 967–981.
Qianqian, W., Shuang, M., Hourong, X., Min, Z. and C. Jingmin, (2011). ″Purification and the secondary structure of fucoidanase from Fusarium sp. LD8.″ Evidence Based Complementary Alternative Medicine, 1–8.
Rodríguez-Jasso, R.M., Aguilar-Gonzalez, C.N., Pastrana, L. and J.A. Teixeira, (2008). ″Identification and evaluation of fungal strains with fucoidan degradation potential.″ Proceedings of the 10th International Chemical and Biological Engineering Conference-CHEMPOR 2008 E. C. Ferreira and M. Mota. Braga, Portugal, Universidade do Minho. 2106–2109
Rodríguez-Jasso, R.M., Solange, I.M., Lorenzo, P., Cristóbal, N.A. and A.T. José, (2010). ″Fucoidan-Degrading Fungal Strains: Screening, Morphometric Evaluation, and Influence of Medium Composition.″ Applied Biochemistry & Biotechnology, 162: 2177–2188.
Sakai, T., Kimura, H. and I. Kato, (2002). ″A marine strain of a flavobacteriaceae utilize brown seaweeds fucoidan.″ Marine Biotechnology, 4: 399-405.
Schaeffer, D.J. and V.S. Krylov, (2000). ″Anti-HIV activity of extracts and compounds from algae and cyanobacteria.″ Ecotoxicology & Environmental Safety, 45: 208–227.
Shiwei, H., Jinhui, W., Jingfeng, W., Shijie, L., Wei, J. and L. Yu, (2017). ″Renoprotective effect of fucoidan from Acaudina molpadioides in streptozotocin/high fat diet-induced type 2 diabetic mice.″ Journal of Functional Foods 31: 123-130.
Shvetsova, S.V., Zhurishkina, E.V., Bobrov, K.S., Ronzhina, N.L., Lapina, I.M., Ivanen, D.R., Gagkaeva, T. Y. and A.A. Kulminskaya, (2014). ″The novel strain Fusarium proliferatum LE1 (RCAM02409) produces a-L-fucosidase and arylsulfatase during the growth on fucoidan.″ Journal of Basic Microbiology, 54: 1 –9
Silchenko, A.S., Kusaykin, M.I., Kurilenko, V.V., Zakharenko, A.M., Isakov, V.V., Zaporozhets, T.S., Gazha, A.K. and T. N. Zvyagintseva, (2013). ″Hydrolysis of fucoidan by fucoidanase isolated from the marine bacterium, Formosa algae.″ Marine Drugs, 11:2413–2430
Wang, D., Yun, E.J., Kim, S., Kim, D.H., Seo, N., An, H.J., Kim, J.H., Cheong, N.Y. and K.H. Kim, (2016). ″Efficacy of acidic pretreatment for the saccharification and fermentation of alginate from brown macroalgae.″ Bioprocess & Biosystem Engineering, 39: 959–966.
Wang, J., Zhang, Q., Zhang, Z. and Z. Li, (2007). ″Antioxidant activity of sulphated polysaccharide fraction from Laminaria japonica.″ International Journal of Biological Macromolecules, 42: 127-132.
Wu, Q., Zhang, M., Wu, K., Liu, B., Cai, J. and R. Pan, (2011). ″Purification and characteristics of fucoidanase obtained from Dendryphiella arenaria TM94.″ Journal of Applied Phycology, 23; 197–203.
- Abstract Viewed: 69 times
- PDF Downloaded: 28 times