Assessing Dentinal Microcracks in Molar Root Canals Following Preparation with OneShape, 2Shape, and OneCurve Rotary Files: An In Vitro Micro-CT Study
Journal of Dental School,
Vol. 43 No. 2 (2025),
30 April 2025
,
Page 88-94
https://doi.org/10.22037/jds.v43i2.46398
Abstract
Objective(s): Microcrack formation during endodontic treatment is a major concern because it can compromise the success of the procedure. Although rotary nickel-titanium files have become the standard of care for canal shaping, there is some concern that they may be more likely to cause microcracks than other types of files. This study evaluated the incidence of microcracks in mandibular and maxillary molars after using three different rotary file systems. Methods: This in vitro experimental study investigated 51 canals in 30 periodontally compromised mandibular and maxillary molars. Prior to root canal instrumentation, micro-computed tomography (micro-CT) imaging was conducted. The canals were subsequently categorized into three groups (N=17), prepared using the OneShape, 2Shape, and OneCurve rotary file systems; and re-evaluated using micro-CT. Statistical analysis was performed using the Shapiro-Wilk and Levene's tests. Intergroup comparisons were conducted using ANOVA with Tukey's honestly significant difference (HSD) post-hoc tests. Categorical data were analyzed using chi-square tests at p<0.05. Results: There was a statistically significant difference in the mean number of microcracks after canal preparation among the groups (p = 0.02). A statistically significant increase in crack formation was observed in the OneShape group compared to both the OneCurve group (p < 0.001) and the 2Shape group (p < 0.001).
Conclusion: During canal preparation, rotary files can lead to the development of dentinal microcracks. OneShape rotary files caused a higher incidence of microcracks than the 2Shape and OneCurve files.
- Root Canal Preparation
- Root canal therapy
- Tooth Fractures
How to Cite
References
Tonelli SQ, de Toubes KS, Pereira AM, de Oliveira Miranda AC, Fernandes LL, Nunes E, Silveira FF. Dentinal microcracks induced by endodontic procedures: A scientometric and bibliometric analysis. J Conserv Dent. 2022;25(1):78-87. doi: 10.4103/jcd.jcd_469_21
Panda A, Shah K, Budakoti V, Dere K, Virda M, Jani J. Evaluation of microcrack formation during root canal preparation using hand, rotary files and self-adjusting file in primary teeth: An in vitro study. J Dent Res Dent Clin Dent Prospects. 2021;15(1):35-41. doi: 10.34172/joddd.2021.007.
Mohamed R, Majid S, Faiza A. Root Microcracks Formation during Root Canal Instrumentation Using Reciprocating and Rotary Files. J Contemp Dent Pract. 2021;22(3):259-263.
Iosif L, Dimitriu B, Niţoi DF, Amza O. Endodontic Dentistry: analysis of Dentinal Stress and Strain Development during Shaping of Curved Root Canals. Healthcare (Basel). 2023;11(22):2918. doi: 10.3390/healthcare11222918
Haueisen H, Gärtner K, Kaiser L, Trohorsch D, Heidemann D. Vertical root fracture: prevalence, etiology, and diagnosis. Quintessence Int. 2013;44(7):467-74. doi: 10.3290/j.qi.a29715
Yoldas O, Yilmaz S, Atakan G, Kuden C, Kasan Z. Dentinal microcrack formation during root canal preparations by different NiTi rotary instruments and the self-adjusting file. J Endod. 2012;38(2):232-5. doi:10.1016/j.joen.2011.10.011
Ceyhanli KT, Erdilek N, Tatar I, Celik D. Comparison of ProTaper, RaCe and Safesider instruments in the induction of dentinal microcracks: a micro-CT study. Int Endod J. 2016;49(7):684-9.
Wang M, Gao Y, Deng Q, Gao Y, Song D, Huang D. Assessment of the coronal root canal morphology of permanent maxillary first molars using digital 3D-reconstruction technology based on micro-computed tomography data. J Dent Sci. 2023 ;18(2):586-593. doi: 10.1016/j.jds.2022.08.011
Cassimiro M, Romeiro K, Gominho L, de Almeida A, Costa L, Albuquerque D. Occurence of dentinal defects after root canal preparation with R-phase, M-Wire and Gold Wire instruments: a micro-CT analysis. BMC oral health. 2017;17(1):93. doi: 10.1186/s12903-017-0387-0
De-Deus G, Belladonna FG, Souza EM, Silva EJ, Neves Ade A, Alves H, et al. Micro-computed Tomographic Assessment on the Effect of ProTaper Next and Twisted File Adaptive Systems on Dentinal Cracks. J Endod. 2015;41(7):1116-9. doi: 10.1016/j.joen.2015.02.012
Hargreaves KM, Cohen S. Cohen's pathways of the pulp. Elsevier; 2021, Jan 1.p: 865
Valle AD, Dotto L, Morgental RD, Pereira-Cenci T, Pereira GK, Sarkis-Onofre R. Influence of root canal preparation on formation of dentinal microcracks: a systematic review. Braz Dent J. 2020; 31:201-20. doi: 10.1590/0103-6440202002652
Bürklein S, Benten S, Schäfer E. Shaping ability of different single-file systems in severely curved root canals of extracted teeth. Int Endod J. 2013;46(6):590-7. doi: 10.1111/iej.12037
Staffoli S, Grande NM, Plotino G, Özyürek T, Gündoğar M, Fortunato L, et al. Influence of environmental temperature, heat-treatment and design on the cyclic fatigue resistance of three generations of a single-file nickel-titanium rotary instrument. Odontology. 2019;107(3):301-7. doi: 10.1007/s10266-018-0399-5
Singh R, Dubey S, Singh P, Samant PS, Saha SG. Comparative evaluation of stress distribution against the root canal wall at three different levels using novel NiTi rotary files–A finite element analysis. J Conserv Dent Endod. 2024 Jan;27(1):62-67. doi: 10.4103/JCDE.JCDE_96_23
Alghamdi A, Alsofi L, Balto K. Effects of a Novel NiTi thermomechanical treatment on the geometric features of the prepared root canal system. Materials (Basel). 2020;13(23):5546. doi: 10.3390/ma13235546
Ertuğrul İF. Effect of sodium hypochlorite on the cyclic fatigue resistance: A scanning electron microscopy evaluation. Microsc Res Tech. 2019;82(12):2089-2094. doi: 10.1002/jemt.23381
Saricam E, Kayaoglu G. Comparison of OneShape, 2Shape and One Curve endodontic instruments for debris and irrigant extrusion. Dent Med Probl. 2020 Jul-Sep;57(3):255-259. doi: 10.17219/dmp/119771
Priya NT, Chandrasekhar V, Anita S, Tummala M, Raj TB, Badami V, et al. "Dentinal microcracks after root canal preparation" a comparative evaluation with hand, rotary and reciprocating instrumentation. J Clin Diagn Res. 2014 Dec;8(12):ZC70-2. doi: 10.7860/JCDR/2014/11437.5349
Yang GB, Zhou XD, Zhang H, Wu HK. Shaping ability of progressive versus constant taper instruments in simulated root canals. Int Endod J. 2006 Oct;39(10):791-9. doi: 10.1111/j.1365-2591.2006.01151.x
Yamamura B, Cox TC, Heddaya B, Flake NM, Johnson JD, Paranjpe A. Comparing canal transportation and centering ability of endosequence and vortex rotary files by using micro-computed tomography. J Endod. 2012;38(8):1121-5. doi: 10.1016/j.joen.2012.04.019
Deopujari J, Singh H, Pathak I, Shyamal R, Antony SM, Singh S. Evaluation of Microcracks formed During Root Canal Preparation by Different File Systems Using Micro-computed Tomography: An In Vitro Study. J Contemp Dent Pract. 2022;23(1):74-78.
Dowker SE, Davis GR, Elliott JC. X-ray microtomography: nondestructive three-dimensional imaging for in vitro endodontic studies. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1997;83(4):510-6. doi: 10.1016/s1079-2104(97)90155-4
Bal SS, Pare S, Unnikrishnan A, Shetty D, Kumar HG, Ragher M. Detection of dentinal microcracks in radicular dentin after shaping with XP-endo Shaper, Neoendo flex files, and Hero Shaper using scanning electron microscope: An in vitro study. J Pharm Bioallied Sci. 2020;12(Suppl 1):S259-63. doi: 10.4103/jpbs.JPBS_76_20
Koroth S, Ponnappa KC, Simon EP, Bharath S, Vivek VJ, Raveendran C. Evaluation of root dentinal damage associated with protaper gold, waveone gold, and protaper next during cleaning and shaping procedures: An In vitro analysis. J Pharm Bioallied Sci. 2021;13(Suppl 2):S1098-S1101. doi: 10.4103/jpbs.jpbs_374_21
Pawar AM, Thakur B, Kfir A, Kim HC. Dentinal defects induced by 6 different endodontic files when used for oval root canals: an in vitro comparative study. Restor Dent Endod. 2019;44(3):e31. doi: 10.5395/rde.2019.44.e31
Mariyam R, Khurana NS, Mann JS, Sidhu HS, Sergy A, Mohan M, Khurana N, Mann J, Sidhu HS. Comparative Evaluation of the Incidence of Dentin Microcracks Following Biomechanical Preparation Using Four Different Nickel-Titanium Rotary File Systems: An In Vitro Study. Cureus. 2024 Feb 3;16(2). doi: 10.7759/cureus.53506
Liu R, Hou BX, Wesselink PR, Wu MK, Shemesh H. The incidence of root microcracks caused by 3 different single-file systems versus the ProTaper system. Journal of endodontics. 2013;39(8):1054-6. J Endod. 2013;39(8):1054-6. doi: 10.1016/j.joen.2013.04.013
Fráter M, Jakab A, Braunitzer G, Tóth Z, Nagy K. The potential effect of instrumentation with different nickel titanium rotary systems on dentinal crack formation—An in vitro study. Plos One. 2020 Sep 9;15(9):e0238790. doi: 10.1371/journal.pone.0238790
Capar ID, Ertas H, Ok E, Arslan H, Ertas ET. Comparative study of different novel nickel-titanium rotary systems for root canal preparation in severely curved root canals. J Endod. 2014;40(6):852-6. doi: 10.1016/j.joen.2013.10.010
Arumugam S, Yew HZ, Baharin SA, Qamaruz Zaman J, Muchtar A, Kanagasingam S. Micro‐computed tomography evaluation of dentinal microcracks following canal preparation with thermomechanically heat‐treated engine‐driven files. Aust Endod J. 2021;47(3):520-530. doi: 10.1111/aej.12516
Bayram HM, Bayram E, Ocak M, Uzuner, MB, Geneci F, Celik HH. Micro–computed tomographic evaluation of dentinal microcrack formation after using new heat-treated nickel-titanium systems. Aust Endod J. 2021;47(3):520-530. doi: 10.1111/aej.12516
Aksoy Ç, Keriş EY, Yaman SD, Ocak M, Geneci F, Çelik HH. Evaluation of XP-endo Shaper, Reciproc Blue, and ProTaper Universal NiTi Systems on dentinal microcrack formation using micro–computed tomography. Journal of endodontics. 2019 Mar 1;45(3):338-42. J Endod. 2019 Mar;45(3):338-342. doi: 10.1016/j.joen.2018.12.005
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