Assessing the Cytotoxicity of Materials Used as Bonding Agents in Orthodontic Fixed Retainers
Journal of Dental School,
Vol. 41 No. 3 (2023),
21 Farvardin 2024
,
Page 88-91
https://doi.org/10.22037/jds.v41i3.43627
Abstract
Objectives Fixed orthodontic retainers are pivotal in determining the success of treatment outcomes. However, the bonding materials utilized in their construction are not completely inert. These materials may release bisphenol A (BPA), a compound associated with various health risks. This study aimed to evaluate the cytotoxicity of potential bonding agents used in fixed orthodontic retainers.
Methods In this experimental study, human gingival fibroblasts were cultured and subjected to various dental materials. These materials included acrylic resin (representing removable retainers), Transbond LR, and Enlight LV (which are common adhesives for fixed retainers), as well as Gradia Direct, Direct Flo, and Herculite XRV (commonly used in restorative composites). A control group was also exposed to normal saline. Discs measuring 0.5×1×1 mm were prepared from these materials. The viability of the fibroblasts was assessed using the MTT assay, and a statistical analysis (one-way analysis of variance) was conducted to compare the groups at a significance level of 0.05.
Results The results of statistical analysis indicated no significant difference between the groups in terms of cytotoxicity for human gingival fibroblasts (p= 0.71).
Conclusion None of the tested materials negatively impacted the viability of human gingival fibroblasts. These results provide valuable clinical insights, reassuring orthodontic practitioners about the safety profile of these commonly used materials in the context of fixed orthodontic retention and restorative procedures.
- Fixed orthodontic appliances
- Orthodontic
- Cytotoxic agent
- Bonding
- Dental
How to Cite
References
Alassiry AM. Orthodontic retainers: A contemporary overview. J Contemp Dent Pract.. 2019; 20: 857-62.
Proffit WR, Fields HW, Larson B, Sarver DM. Contemporary orthodontics. Philadelphia: Elsevier. 2019; Chap18: 579-99.
Segner D, Heinrici B. Bonded retainers–clinical reliability. J Orofac Orthop. 2000; 61: 352-8.
Marinelli G, Inchingolo A, Inchingolo A, Malcangi G, Limongelli L, Montenegro V, et al. White spot lesions in orthodontics: prevention and treatment. A descriptive review. J Biol Regul Homeost Agents. 2021; 35: 227-40.
Chantarawaratit P-o, Yanisarapan T. Exposure to the oral environment enhances the corrosion of metal orthodontic appliances caused by fluoride-containing products: Cytotoxicity, metal ion release, and surface roughness. Am J Orthod Dentofacial Orthop. 2021; 160: 101-12.
Kloukos D, Sifakakis I, Voutsa D, Doulis I, Eliades G, Katsaros C, et al. BPA qualtitative and quantitative assessment associated with orthodontic bonding in vivo. Dent Mater. 2015; 31: 887-94.
Malkiewicz K, Turlo J, Marciniuk-Kluska A, Grzech-Lesniak K, Gasior M, Kluska M. Release of bisphenol A and its derivatives from orthodontic adhesive systems available on the European market as a potential health risk factor. Ann Agric Environ Med. 2015; 22.
Eliades T. Bisphenol A and orthodontics: An update of evidence-based measures to minimize exposure for the orthodontic team and patients. Am J Orthod Dentofacial Orthop. 2017; 152: 435-41.
Halimi A, Benyahia H, Bahije L, Adli H, Azeroual M-F, Zaoui F. A systematic study of the release of bisphenol A by orthodontic materials and its biological effects. Int Orthod. 2016; 14: 399-417.
Becher R, Wellendorf H, Sakhi AK, Samuelsen JT, Thomsen C, Bølling AK, et al. Presence and leaching of bisphenol a (BPA) from dental materials. Acta Biomater Odontol Scand. 2018; 4: 56-62.
Sabour A, El Helou M, Roger-Leroi V, Bauer C. Release and toxicity of bisphenol-A (BPA) contained in orthodontic adhesives: A systematic review. Int Orthod. 2021; 19: 1-14.
Jung YS, Ro ST, Kang SW, Lee H, Lee JS, Chae YK, et al. Bisphenol A release from commercially available 3-dimensionally printed resins and human cell apoptosis to bisphenol A: an in-vitro study. J Clin Pediatr Dent. 2023; 47: 89-95.
Sigaroodi F, Shafaei H, Karimipour M, Dolatkhah MA, Delazar A. Aloe vera/collagen mixture induces integrin α1β1 and PECAM-1 genes expression in human adipose-derived stem cells. Adv Pharm Bull. 2019; 9: 662.
Iliadi A, Koletsi D, Papageorgiou SN, Eliades T. Safety considerations for thermoplastic-type appliances used as orthodontic aligners or retainers. A systematic review and meta-analysis of clinical and in-vitro research. Materials. 2020; 13: 1843.
Bationo R, Rouamba A, Diarra A, Beugré‐Kouassi MLA, Beugré JB, Jordana F. Cytotoxicity evaluation of dental and orthodontic light‐cured composite resins. Clin Exp Dent Res. 2021; 7: 40-8.
Eliades T, Voutsa D, Sifakakis I, Makou M, Katsaros C. Release of bisphenol-A from a light-cured adhesive bonded to lingual fixed retainers. Am J Orthod Dentofacial Orthop. 2011; 139: 192-5.
Moreira MR, Matos LG, de Souza ID, Brigante TAV, Queiroz MEC, Romano FL, et al. Bisphenol A release from orthodontic adhesives measured in vitro and in vivo with gas chromatography. Am J Orthod Dentofacial Orthop. 2017; 151: 477-83.
Jedeon K, Loiodice S, Marciano C, Vinel A, Canivenc Lavier MC, Berdal A, et al. Estrogen and bisphenol A affect male rat enamel formation and promote ameloblast proliferation. Endocrinology. 2014; 155: 3365-75.
Jedeon K, De la Dure-Molla M, Brookes SJ, Loiodice S, Marciano C, Kirkham J, et al. Enamel defects reflect perinatal exposure to bisphenol A. Am J Pathol. 2013; 183: 108-18.
Schmalz GH, Widbiller M. Biocompatibility of Amalgam vs Composite-A Review. Oral Health Prev Dent. 2022; 20: 149-56.
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