Mechanical Properties of 3D Printed Reinforced Polycaprolactone Composite Scaffolds
Journal of Dental School, Shahid Beheshti University of Medical Sciences,
Vol. 38 No. 1 (2020),
21 April 2021
,
Page 1-6
https://doi.org/10.22037/jds.v38i1.33348
Abstract
Objectives This paper describes the fabrication of a new porous 3D-printed scaffold composed of polycaprolactone (PCL) and polyether-ether ketone (PEEK) micro-particles for bone tissue engineering (BTE) applications.
Methods In order to improve the compatibility of the reinforcing PEEK powder with polycaprolactone, the PEEK powder was surface-modified by an amino-silane coupling agent. After modification, Fourier-transform infrared spectrometry (FTIR) and differential scanning calorimetry (DSC) were used to investigate the chemical reaction between PEEK and silane coupling agent. In order to increase the compressive modulus of the 3D printed PCL scaffold, 10% silane-modified PEEK was incorporated into the PCL polymeric matrix. Scanning electron microscopy (SEM) was used for cell morphology and attachment evaluation.
Results The results indicated that the silane coupling agent was successfully grafted onto the particle surface. The compressive modulus of PCL scaffold increased by incorporating the silane-modified PEEK, despite having higher porosity, compared with the pure PCL scaffolds. Addition of amino-silane had a positive impact on cell response, and that surface modification led to improved particle dispersion.
Conclusion In conclusion, it seems that the incorporation of surface-modified PEEK micro-particles into the PCL porous scaffold could enhance its mechanical properties, and may be applicable for the management of large bone defects.
- Polyetheretherketone
- Polycaprolactone
- Amino-propyl-triethoxysilane
- Tissue Scaffolds
- Printing, Three-Dimensional
How to Cite
References
Gao C. Polymeric Biomaterials for Tissue Regeneration: From Surface/Interface Design to 3D Constructs: Springer; 2016;1-37.
Gomez-Lizarraga KK, Flores-Morales C, Del Prado-Audelo ML, Alvarez-Perez MA, Pina-Barba MC, Escobedo C. Polycaprolactone- and polycaprolactone/ceramic-based 3D-bioplotted porous scaffolds for bone regeneration: A comparative study. Mater Sci Eng C Mater Biol Ap. 2017;79:326-35.
Bai H, Xiu H, Gao J, Deng H, Zhang Q, Yang M, et al. Tailoring impact toughness of poly(L-lactide)/poly(epsilon-caprolactone) (PLLA/PCL) blends by controlling crystallization of PLLA matrix. ACS Appl. Mater. Interfaces. 2012;4(2):897-905.
Thi Hiep N, Chan Khon H, Dai Hai N, Byong-Taek L, Van Toi V, Thanh Hung L. Biocompatibility of PCL/PLGA-BCP porous scaffold for bone tissue engineering applications. J Biomater Sci Polym Ed. 2017;28(9):864-78.
Sun H, Mei L, Song C, Cui X, Wang P. The in vivo degradation, absorption and excretion of PCL-based implant. Biomaterials. 2006;27(9):1735-40.
M K, Devine J.N. PEEK Biomaterials in Trauma, Orthopedic, and Spinal Implants. Biomaterials. 2007;28(32):4845–69.
Pruitt L, Furmanski J. Polymeric biomaterials for load-bearing medical devices. JOM. 2009;61(9):14-20.
Uddin MN, Dhanasekaran PS, Asmatulu R. Mechanical properties of highly porous PEEK bionanocomposites incorporated with carbon and hydroxyapatite nanoparticles for scaffold applications. Prog Biomater 2019;9:211-21.
Vaezi M, Black C, Gibbs DM, Oreffo RO, Brady M, Moshrefi-Torbati M, et al. Characterization of New PEEK/HA Composites with 3D HA Network Fabricated by Extrusion Freeforming. Molecules. 2016;21(6):687.
Raucci MG, Demitri C, Soriente A, Fasolino I, Sannino A, Ambrosio L. Gelatin/nano-hydroxyapatite hydrogel scaffold prepared by sol-gel technology as filler to repair bone defects. J Biomed Mater Res Part A. 2018;106(7):2007-19.
Amemiya Y, Hatakeyama A, Shimamoto N. Aminosilane multilayer formed on a single-crystalline diamond surface with controlled nanoscopic hardness and bioactivity by a wet process. Langmuir. 2008;25(1):203-9.
Toworfe G, Bhattacharyya S, Composto R, Adams CS, Shapiro I, Ducheyne P. Effect of functional end groups of silane self‐assembled monolayer surfaces on apatite formation, fibronectin adsorption and osteoblast cell function. J Tissue Eng Regen Med. 2009;3(1):26-36.
Nourmohammadi J, Hajibabaei T, Amoabediny G, Jafari SH, Salehi-Nik N. Aminosilane Layer Formation Inside the PDMS Tubes Improves Wettability and Cytocompatibility of Human Endothelial Cells. Trends Biomater. Artif. Organs, 2015; 29(2), 123-31.
Kurniawan A, Gunawan F, Nugraha AT, Ismadji S, Wang MJ. Biocompatibility and drug release behavior of curcumin conjugated gold nanoparticles from aminosilane-functionalized electrospun poly(N-vinyl-2-pyrrolidone) fibers. Int J Pharm. 2017;516(1-2):158-69.
Horwitz JA, Shum KM, Bodle JC, Deng M, Chu CC, Reinhart-King CA. Biological performance of biodegradable amino acid-based poly(ester amide)s: Endothelial cell adhesion and inflammation in vitro. J Biomed Mater Res Part A. 2010;95(2):371-80.
Kurniawan A, Gunawan F, Nugraha AT, Ismadji S, Wang MJ. Biocompatibility and drug release behavior of curcumin conjugated gold nanoparticles from aminosilane-functionalized electrospun poly (N-vinyl-2-pyrrolidone) fibers. Int J Pharm. 2017;516(1-2):158-69.
Zhu Y, Gao C, Liu X, Shen J. Surface modification of polycaprolactone membrane via aminolysis and biomacromolecule immobilization for promoting cytocompatibility of human endothelial cells. Biomacromolecules. 2002;3(6):1312-9.
Arima Y, Iwata H. Effects of surface functional groups on protein adsorption and subsequent cell adhesion using self-assembled monolayers. J. Mater. Chem. 2007;17(38):4079-87.
Glass NR, Tjeung R, Chan P, Yeo LY, Friend JR. Organosilane deposition for microfluidic applications. Biomicrofluidics. 2011;5(3):36501-17.
Salehi M, Farzamfar S, Bastami F, Tajerian R. Fabrication and characterization of electrospun PLLA/collagen nanofibrous scaffold coated with chitosan to sustain release of aloe vera gel for skin tissue engineering. Biomed Eng (Singapore). 2016;28(05):1650035.
Mammeri F, Bourhis EL, Rozes L, Sanchez C. Mechanical properties of hybrid organic–inorganic materials. J Mater Chem. 2005;15(35-36):3787-811.
Amdjadi P, Ghasemi A, Najafi F, Nojehdehian H. Pivotal role of filler/matrix interface in dental composites. Biomed Res. 2017;28(3):1054-65.
Wang Z, Wang C, Li C , Qin Y, Zhong L, Chen B, et al. Analysis of factors influencing bone ingrowth into three dimensional printed porous metal scaffolds: a review, J. Alloys Compd. 2017;71(7): 271-85.
Zhang B, Pei X, Zhou C, Fan Y, Jiang Q , Ronca A, et al. The biomimetic design and 3D printing of customized mechanical properties porous Ti6Al4V scaffold for load-bearing bone reconstruction. Mater Des. 2018; (152): 30-9.
Zheng T, Xi H, Wang Z, Zhang X, Wang Y, Qiao Y, et al. The curing kinetics and mechanical properties of epoxy resin composites reinforced by PEEK microparticles. Polym test. 2020;91(11): 1067-81.
Gervaso F, Sannino A, Peretti M. The biomaterialist's task: scaffold biomaterials and fabrication technologies. Joints. 2013;1 (3):130-7.
Lv Q, Nair L, Laurencin CT. Fabrication, characterization, and in vitro evaluation of poly(lactic acid glycolic acid)/nano-hydroxyapatite composite microsphere-based scaffolds for bone tissue engineering in rotating bioreactors. J Biomed Mater Res A. 2009;91(3):679-91.
Khoshroo K, Jafarzadeh Kashi TS, Moztarzadeh F, Tahriri M, Jazayeri HE, Tayebi L, et al. Development of 3D PCL microsphere/TiO2 nanotube composite scaffolds for bone tissue engineering. Mater Sci Eng C Mater Biol Appl. 2017;70: 586-98.
Ang HY, Toong D, Chow WS, Seisilya W, Wu W, Wong P, et al. Radiopaque Fully Degradable Nanocomposites for Coronary Stents. Sci Rep. 2018; 8:17409.
Tatullo M, Marrelli M, Shakesheff KM, White LJ. Dental pulp stem cells: function, isolation and applications in regenerative medicine. J Tissue Eng Regen Med. 2015; 9(11):1205-16.
Chang HI, Wang Y. Cell responses to surface and architecture of tissue engineering scaffolds. InRegenerative medicine and tissue engineering-cells and biomaterials 2011 Aug 29. InTechOpen.
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