Asparagus Officinalis: An Herbal Candidate for an Intraoral Healing Mouthwash In-vitro healing effects of Asparagus officinalis
Journal of Dental School, Shahid Beheshti University of Medical Sciences,
Vol. 38 No. 1 (2020),
21 April 2021
,
Page 31-36
https://doi.org/10.22037/jds.v38i1.32529
Abstract
Objectives Asparagus officinalis (A. officinalis) extract has several bioactive ingredients. This study assessed the healing effects of A. officinalis methanolic extract.
Methods In this experimental study, after preparing the methanolic extract of A. officinalis with a concentration of 100 , its bioactive ingredients were determined using high-performance liquid chromatography (HPLC) and then its cytotoxicity was assessed using the methyl thiazolyl tetrazolium (MTT) assay. Five experimental groups with 25 samples were assessed as follows: (I)human gingival fibroblast(HGFs) cultured in high-glucose Dulbecco’s modified Eagle’s medium (DMEM), (II) same as group Ibut with 10 μg/mL methanolic extract of A. officinalis, (III) same as group Ibut with 25μg/mL methanolic extract of A. officinalis, (IV) same as group Ibut with 50 μg/mL methanolic extract of A. officinalis, and (V)same as group Ibut with 100μg/mL methanolic extract of A. officinalis. Cell motility in the control group and group Vwas examined quantitatively using the cell scratch assay at 24 h. We used one-way ANOVA and t-test to analyzethe cytotoxicity of A. officinalis extract and the motility of HGFs, respectively.
Results The MTT assay showed no significant difference in cell viability among the experimental groups (P=0.07). A remarkable cellular wound closure equal to 60.85% was noted after 24 h.
Conclusion The methanolic extract of A. officinalis with a concentration of 100 μg⁄mL showed significant healing effects on an experimental scratch setup of HGFs.
- Wound Healing
- Fibroblasts
- Asparagus Plant
- Herbal Medicine
How to Cite
References
Bjarnsholt T. The role of bacterial biofilms in chronic infections. APMIS Suppl. 2013 May;(136):1-51.
Zaleckiene V, Peciuliene V, Brukiene V, Drukteinis S. Traumatic dental injuries: etiology, prevalence and possible outcomes. Stomatologija. 2014;16(1):7-14.
Politis C, Schoenaers J, Jacobs R, Agbaje JO. Wound healing problems in the mouth. Front. physiol. 2016;7:507.
Porter SR, Mercadente V, Fedele S. Oral manifestations of systemic disease. Bdj Team. 2018;5:18012.
Scully C, Felix D. Oral medicine—Update for the dental practitioner Aphthous and other common ulcers. Br Dent J. 2005 Sep;199(5):259-64.
Bilodeau EA, Lalla RV. Recurrent oral ulceration: Etiology, classification, management, and diagnostic algorithm. Periodontology 2000. 2019;80(1):49-60.
Singla S, Malhotra R, Nd S, Saxena S. Antibacterial efficacy of mouthwash prepared from pomegranate, grape seed and Guava extracts against oral streptococci: An in vivo study. J Clin Pediatr Dent. 2018;42(2):109-13.
Bekut M, Brkić S, Kladar N, Dragović G, Gavarić N, Božin B. Potential of selected Lamiaceae plants in anti (retro) viral therapy. Pharmacol Res. 2018;133:301-14.
Mardani M, Badiee P, Gharibnavaz M, Jassebi A, Jafarian H, Ghassemi F. Comparison of anti-Candida activities of the ancient plants Lawsonia inermis and Ziziphus spina christi with antifungal drugs in Candida species isolated from oral cavity. J Conserv Dent. 2018;21(4):359- 362.
Gomes VTS, Nonato Silva Gomes R, Gomes MS, Joaquim WM, Lago EC, Nicolau RA. Effects of Matricaria recutita (L.) in the treatment of oral mucositis. Sci. World J. 2018; 2018:4392184.
Negi JS, Singh P, Joshi GP, Rawat MS, Bisht VK. Chemical constituents of Asparagus. Pharmacogn. Rev. 2010;4(8):215-20.
Semaming Y, Pannengpetch P, Chattipakorn SC, Chattipakorn N. Pharmacological properties of protocatechuic acid and its potential roles as complementary medicine.Evid Based Complement Alternat Med. 2015;2015:593902.
Kakkar S, Bais S. A review on protocatechuic acid and its pharmacological potential. ISRN Pharmacol. 2014;2014.
Al-Snafi AE. The pharmacological importance of Asparagus officinalis-A review. Pharm Biol. 2015;5(2):93-8.
Wild T, Rahbarnia A, Kellner M, Sobotka L, Eberlein T. Basics in nutrition and wound healing. Nutrition. 2010;26(9):862-6.
Gopalakrishnan A, Ram M, Kumawat S, Tandan S, Kumar D. Quercetin accelerated cutaneous wound healing in rats by increasing levels of VEGF and TGF-β1. Indian J Exp Biol. 2016;54(3):187-95..
Ehrlich HP, Tarver H, Hunt TK. Effects of vitamin A and glucocorticoids upon inflammation and collagen synthesis. Ann Surg. 1973;177(2):222.
Kato-Noguchi H, Nakamura K, Ohno O, Suenaga K, Okuda N. Asparagus decline: Autotoxicity and autotoxic compounds in asparagus rhizomes. J Plant Physiol. 2017;213:23-29.
Grada A, Otero-Vinas M, Prieto-Castrillo F, Obagi Z, Falanga V. Research techniques made simple: analysis of collective cell migration using the wound healing assay. J Invest. Dermatol. 2017;137(2):e11-e6.
Lee JW, Lee JH, Yu IH, Gorinstein S, Bae JH, Ku YG. Bioactive compounds, antioxidant and binding activities and spear yield of Asparagus officinalis L. Plant Foods Hum Nutr. 2014;69(2):175-81.
Kapoor M, Howard R, Hall I, Appleton I. Effects of epicatechin gallate on wound healing and scar formation in a full thickness incisional wound healing model in rats. Am J Pathol. 2004;165(1):299-307.
Zhou YD, Kim YP, Li XC, Baerson SR, Agarwal AK, Hodges TW, et al. Hypoxia-inducible factor-1 activation by (−)-epicatechin gallate: potential adverse effects of cancer chemoprevention with high-dose green tea extracts. J Nat Prod.2004;67(12):2063-9.
Kakkar S, Bais S. A review on protocatechuic acid and its pharmacological potential. ISRN Pharmacol. 2014 Mar;2014:952943.
Heleno SA, Martins A, Queiroz MJR, Ferreira IC. Bioactivity of phenolic acids: Metabolites versus parent compounds: A review. Food Chem. 2015;173:501-13.
Nicolaus C, Junghanns S, Hartmann A, Murillo R, Ganzera M, Merfort I. In vitro studies to evaluate the wound healing properties of Calendula officinalis extracts. J Ethnopharmacol. 2017;196:94-103.
Nicolaus C, Junghanns S, Hartmann A, Murillo R, Ganzera M, Merfort I. In vitro studies to evaluate the wound healing properties of Calendula officinalis extracts. JEthnopharmacol.2017;196:94-103.
Liu CL, Tam JCW, Sanders AJ, Ko CH, Fung KP, Leung PC, et al. Molecular angiogenic events of a two‐herb wound healing formula involving MAPK and A kt signaling pathways in human vascular endothelial cells. Wound Repair Regen. 2013;21(4):579-87.
Torabi M, Afzali M, Aminizadeh M, Karimiafshar H. Study on the Effect of Essence and Extract of Thymus caramanicus Jalas on the Process of Oral Wound Healing in Rats. Asian j. biomed. pharm. 2015;5(44):1-5.
Nirmala S, Karthiyayini T. Wound healing activity on the leaves of Achillea millefolium L. by excision, incision, and dead space model on adult wistar albino rats. Int Res J Pharm. 2011;2(3):240-5.
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