Immunohistochemical Analysis of E-Cadherin Expression in Pleomorphic Adenoma and Mucoepidermoid Carcinoma of Salivary Glands
Journal of Dental School,
Vol. 31 No. 2 (2013),
27 April 2013
,
Page 104-109
https://doi.org/10.22037/jds.v31i2.29092
Abstract
Objective: E-cadherin is a classic cadherin that plays a key role in epithelial cell adhesion. This protein is being referred to as the suppressor of proliferation and invasion. Limited studies have investigated E-cadherin expression in salivary gland neoplasms. This study sought to assess the expression of E-cadherin and its possible role in progression and invasion of salivary gland neoplasms.
Methods: In this retrospective cross-sectional study, 15 samples of pleomorphic adenoma (PA) and
9 samples of mucoepidermoid carcinoma (MEC) were immunohistochemically stained for evaluation of E-cadherin expression. Degree of staining was calculated as the percentage of positively stained cell membranes out of a minimum of 1000 neoplastic cells.
Results: In normal salivary gland specimens, intense membrane staining was observed around the acinar mucous and serous cells as well as the ductal cells. Myoepithelial cells were negative. In PA, intense staining was noted along the membrane of attached cells forming the ducts, islands, cellular cords and cellular sheets but the stromal myoepithelial cells were negative. In MEC, epidermoid and intermediate cells showed intense membrane staining. Mucous cells also showed membrane staining. After statistical analysis, the percentage of positive cells was found to be 82.56±11.66 and 67.4±7.24 in MEC and PA, respectively. This difference was not statistically significant (P>0.05).
Conclusion: E-cadherin expression was not a suitable marker for differentiation of PA from MEC. It was only correlated with cell phenotype.
- Cadherin
- E-cadherin
- Immunohistochemistry
- Mucoepidermoid carcinoma
- Pleomorphic adenoma
- Salivary gland neoplasms
How to Cite
References
Dultra FK, Barros AC, Schaer-Barbosa H, Figueiredo AL, Gurgel CA, Ramos EA, et al. Immunohistochemical assessment of CD1a-positive langerhans cells and their relationship with E-cadherin in minor salivary gland tumors. J Oral Pathol Med 2012; 41: 47-53.
Sengüven B, Oygür T. Investigation of interleukin-1 alpha and interleukin-6 expression and interleukin-1 alpha gene polymorphism in keratocystic odontogenic tumors and ameloblastomas. Med Oral Pathol Oral Cir Bucal 2011; 16: 467-472.
Morais Mde L, Azevedo PR, Carvalho CH, Medeiros L, Lajus T, Costa Ade L. Clinicopathological study of salivary gland tumors: an assessment of 303 patients. Cad Saude Publica 2011; 27:1035-1040.
Ito FA, Jorge J, Vargas PA, Lopes MA: Histopathological findings of pleomorphic adenomas of the salivary glands. Med Oral Pathol Oral Cir Bucal 2009; 14: 57-61.
Shieh YS, Chang LC, Chiu KC, Wu CW, Lee HC. Cadherin and catenin expression in mucoepidermoid carcinoma: correlation with histopathologic grade, clinical stage, and patient outcome. J Oral Pathol Med 2003; 32: 297-304.
Economopoulou P, Hanby A, Odell EW. Expression of E-cadherin, cellular differentiation and polarity in epithelial salivary neoplasms. Oral Oncol 2000; 36: 515-518.
Lopes FF, da Costa Miguel MC, Pereira AL, da Cruz MC, de Almeida Freitas R, Pinto LP, et al. Changes in immunoexpression of E-cadherin and beta-catenin in oral squamous cell carcinoma with and without nodal metastasis. Ann Diagn Pathol 2009; 13: 22-29.
Hakim SG, Kosmehl H, Sieg P, Trenkle T, Jacobsen HC, Attila Benedik G, et al. Altered expression of cell- cell adhesion molecules β-catenin/E-cadherin and related Wnt-signaling pathway in sporadic and syndromal keratocystic odontogenic tumors. Clin Oral Investig 2011; 15: 321-328.
Yoo J, Park S, Kang CS, Kang SJ, Kim BK. Expression of E-cadherin and p53 proteins in human soft tissue sarcomas. Arch Pathol Lab Med 2002; 126: 33-38.
Song Y, Oda Y, Hori M, Kuroiwa K, Ono M, Hosoi F et al. N-myc downstream regulated gene-1
⁄ Cap43 may play an important role in malignant progression of prostate cancer, in its close association with E-cadherin. Hum Pathol 2010; 41: 214-222.
Facina G, Lopes-Costa PV, Dos Santos AR, De Vasconcelos-Valença RJ, Pinho-Sobral AL, Ferreira-Filho CP, et al. Immunohistochemical expression of E-cadherin in sclerosing adenosis, ductal carcinoma in situ and invasive ductal carcinoma of the breast. Diagn Cytopathol 2010; 38:235-238.
Gagliardi G, Kandemir O, Liu D, Guida M, Benvestito S, Ruers TG, et al. Changes in E-cadherin immunoreactivity in the adenoma- carcinoma sequence of the large bowel. Virchows Arch 1995; 426: 149-154.
Prabhu S, Kaveri H, Rekha K: Benign; malignant salivary gland tumors:comparison of immunohistochemical expression of e-cadherin. Oral Oncol 2009; 45: 594-599.
Patel RS, Rose B, Bawdon H, Hong A, Lee SC, Fredericks S, et al. Cyclin D1 and p16 expression in pleomorphic adenoma and carcinoma ex pleomorphic adenoma of parotid gland. Histopathology 2007; 51: 691-696.
Smith ME, Pignatelli M. The molecular histology of neoplasia: the role of the cadherin/catenin complex. Histopathology 1997; 31: 107-117.
Andreadis D, Epivatianos A, Mireas G, Nomikos A, Poulopous A, Yiotakis J. Immunohistochemical detection of E-cadherin in certain types of salivary gland tumors. J Laryngol Otol 2006; 120: 298-304.
Furuse C, Cury PR, Altemani A, dos Santos Pinto D Jr, de Araujo NS, de Araujo VC. Beta- catenin and E-cadherin expression in salivary gland tumors. Int J Surg Pathol 2006; 14: 212-217.
Tsubochi H, Suzuki T, Suzuki S, Ohashi Y, Ishibashi S, Moriya T, et al. Immunohistochemical study of basaloid squamous cell carcinoma, adenoid cystic and mucoepidermoid carcinoma in the upper aerodigestive tract. Anticancer Res 2000; 20: 1205- 1211.
Zhang ZY, Wu YQ, Zhang WG, Tian Z, Cao J. The expression of E-cadherin-catenin complex in adenoid cystic carcinoma of salivary glands. Chin J Dent Res 2000; 3: 36-39.
Zhang CY, Mao L, Li L, Tian Z, Zhou XJ, Zhang ZY, et al. Promoter methylation as a common mechanism for inactivation E-cadherin in human salivary gland adenoid cystic carcinoma. Cancer 2007; 110: 87-95.
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