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  3. Vol. 33 No. 1 (2015): Winter
  4. Original Article

Vol. 33 No. 1 (2015)

March 2015

Destructive effects of citric acid, lactic acid and acetic acid on primary enamel microhardness

  • Ali Nozari
  • Afsaneh Rahmati
  • Zahra Shamsaei
  • Anahita Pour Hashemi
  • Mohammad-Karim Layeghnejad
  • Sara Zamaheni

Journal of Dental School, Vol. 33 No. 1 (2015), 7 March 2015 , Page 66-73
https://doi.org/10.22037/jds.v33i1.24751 Published: 2019-03-11

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Abstract

Objective: This study aimed to assess the destructive effects of citric acid, lactic acid and acetic acid produced from the fermentation of foods on primary teeth enamel.

Methods: This in vitro, experimental study was conducted on 24 sound primary teeth. The teeth  were polished with a fine abrasive paper under running water. Tooth pieces measuring 3×4×3mm were cut out of the teeth and stored in 100% humidity until the experiment. The specimens were divided into 3 groups (n=8) and immersed in acetic acid, citric acid and lactic acid, respectively. The enamel microhardness of specimens was measured by Vickers microhardness tester at baseline and 5 and 30min after immersion in the freshly prepared acid solutions.

Results: Repeated measures ANOVA showed that the effect of immersion time on microhardness was significant (p<0.001). Pairwise comparison among 0, 5 and 30 minutes time points using Bonferroni adjustment showed significant differences in microhardness at different time points (p<0.001). Evaluation of the effect of type of acid on microhardness revealed that the microhardness was not significantly different in the three groups of acids (p=0.915). Among the three understudy acids, only the reduction in microhardness from time 0 to 30 minutes was significantly different between lactic acid and acetic acid (p=0.042).

Conclusion: Citric acid, lactic acid and acetic acid were all capable of demineralization and reduction of enamel microhardness. A significant difference existed in the demineralization potential of acids (the highest for lactic acid). However, this effect was more significant early after exposure.
Keywords:
  • Acid
  • Enamel
  • Fermentation
  • Hardness
  • Primary teeth
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How to Cite

Nozari, A., Rahmati, A., Shamsaei, Z., Pour Hashemi, A., Layeghnejad, M.-K., & Zamaheni, S. (2019). Destructive effects of citric acid, lactic acid and acetic acid on primary enamel microhardness. Journal of Dental School, 33(1), 66–73. https://doi.org/10.22037/jds.v33i1.24751
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