Antibacterial effect of morphous (poly-crystalline) and amorphous (glass) nano-bioactive glass 45S5 on Streptococcus mutans
Journal of Dental School, Shahid Beheshti University of Medical Sciences,
Vol. 33 No. 2 (2015),
11 March 2019
,
Page 138-144
https://doi.org/10.22037/jds.v33i2.24733
Abstract
Objective: Bioactive glass 45S5 is a surface reactive glass-ceramic biomaterial, developed in 1969. BAG 45S5 with particle size of 20-60 nm has the ability of bone regeneration, broad spectrum antibacterial effect, repairs and replaces diseased or damaged bone. The aim of this study was to evaluate the antibacterial activity and determine MIC and MBC values of nano-BAG45S5 on Streptococcus mutans.
Methods: In this study the in vitro Antibacterial activity of polycrystalline and glass forms of nano- BAG 45S5 was evaluated. Bacterial susceptibility to test materials was examined by antibiogram test. Afterwards MIC and MBC assays were conducted via broth dilution, disc diffusion and colony count methods.
Results: Despite amorphous nano-BAG 45S5, poly-crystalline form had antibiogram negative test result. In broth dilution test, the optical absorbance of test dilution of 50mcg/ml and higher concentrations were equal to negative control’s optical absorbance and their inhibitory zone diameter were measured 10.0mm in disc diffusion test. No colony was observed on the culture media of test dilution of 200mcg/ml and higher concentrations.
Conclusion: Streptococcus mutans (ATCC 35668) is not susceptible to poly-crystalline nano- BAG45S5. Amorphous nano-BAG45S5 is bacteriostatic against Streptococcus mutans. MIC and MBC values for amorphous nano-BAG45S5 were 50 ppm and 200 ppm, respectively.
- MBC
- MIC
- Morphology
- Nano-BAG45S5
- Streptococcus mutans
How to Cite
References
Jones JR. Review of bioactive glass: from Hench to hybrids. Acta Biomater 2013; 9: 4457-4486.
Hench LL. The story of Bioglass. J Mater Sci Mater Med 2006; 17: 967-978.
Verrier S, Blaker JJ, Maquet V, Hench LL, Boccaccini AR. PDLLA/Bioglass composites for soft- tissue and hard-tissue engineering: an in vitro cell biology assessment. Biomaterials 2004; 25: 3013-3021.
Bendall SP, Gaies M, Frondoza C, Jinnah RH, Hungerford DS. Effect of particulate bioactive glass on human synoviocyte cultures. J Biomed Mater Res 1998; 41: 392-7.
Munukka E, Leppäranata O, Korkeamäki M, Vaahtio M, Peltola T, Zhang D, et al. Bactericidal effects of bioactive glasses on clinically important aerobic bacteria. J Mater Sci Mater Med 2008; 19: 27-32.
Lepparänata O, Vaahtio M, Peltola T, Zhang D, Hupa L, Hupa M, et al. Antibacterial effect of bioactive glasses on clinically important anaerobic bacteria in Vitro. J Mater Sci Mater Med 2008; 19: 547-551.
Allan I, Newman H, Wilson M. Antibacterial activity of particulate bioglass against supra- and subgingival bacteria. Biomaterials 2001; 22: 1683-1687.
Allan I, Newman H, Wilson M. Particulate bioglass reduces the viability of bacterial biofilms formed on its surface in an in vitro model. Clin Oral Implants Res 2002; 13: 53-58.
Pratten J, Nazhat SN, Blaker JJ, Boccaccini AR. In vitro attachment of Staphylococcus epidermidis to surgical sutures with and without Ag-containing bioactive glass coating. J Biomater Appl 2004; 19: 47-57.
Sepulveda P, Jones JR, Hench LL. In vitro dissolution of melt-derived 45S5 and sol-gel derived 58S bioactive glasses. J Biomed Mater Res 2002; 61: 301-311.
Hench LL. Bioceramics: from concept to clinic. J Am Ceram Soc 1991; 74: 1487-1510.
Hu S, Chang J, Liu M, Ning C. Study on antibacterial effect of 45S5 Bioglass. J Mater Sci Mater Med 2009; 20: 281-286.
Waltimo T, Brunner TJ, Vollenweider M, Stark WJ, Zehnder M. Antimicrobial effect of nanometric bioactive glass 45S5. J Dent Res 2007; 86: 754-757.
Wiegand I, Hilpert K, Hancock RE. Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nat Protoc 2008; 3: 163-175.
Bellantone M, Williams HD, Hench LL. Broad-spectrum bactericidal activity of Ag2O-doped bioactive glass. Antimicrob Agents Chemother 2002; 46: 1940-1915.
Nicolas GG, Lavoie MC. [Streptococcus mutans and oral streptococci in dental plaque]. Can J Microbiol 2011; 57: 1-20.
Heifets LB, Lindholm-Levy PJ, Comstock RD. Clarithromycin minimal inhibitory and bactericidal concentrations against Mycobacterium avium. Am Rev Respir Dis 1992; 145: 856- 858.
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