دوره 7 شماره 4 (2020)

Improving Recovery Process of Omega-3 Fatty Acids from a Native Species of Chlorella vulgaris Using Integrated Method

بیوتکنولوژی غذایی کاربردی, دوره 7 شماره 4 (2020), 18 مرداد 2020 , صفحه 273-282
https://doi.org/10.22037/afb.v7i4.29779

چکیده

Arabian-1399.png

Background and Objective: Fish tissue and liver and some microalgae are some of the richest sources of omega-3 (ω3) fatty acids in nature. Annually, more than 4.2 billion tons of algae are cultivated with various goals. Therefore, development of novel ways to extract more ω3-fatty acids from microalgal tissues is a valuable target. In this study, comparisons were carried out between various methods of extraction and purification of ω3-fatty acids from a native species of microalgae.

Material and Methods: Combination method (urea complex and winterization) used as a method for extraction of ω3-fatty acids. For the optimization of combination method, three factors, including temperature of winterization, time of winterization and frequency of extraction with hexane were studied at four various levels and 15 examinations using Design Expert 10.0 software.

Results and Conclusion: Results of optimization included 10 h for time of winterization,
-20 °C for temperature of winterization and six times of extraction with hexane. In optimized conditions, the highest quantity of ω3-fatty acids derived from Chlorella vulgaris at the moisture of 65% was 43.6 mg g-1 fatty acids.

کلمات کلیدی:
  • Omega-3
  • Microalgae
  • Solvent Extraction
  • Urea Complex
  • Unsaturated Fatty Acid
  • Winterization

مراجع

Lenihan-Geels G, Bishop KS, Ferguson LR. Alternative sources of omega-3 fats: can we find a sustainable substitute for fish?. Nutrients. 2013; 5(4): 1301–1315. doi: 10.3390/nu5041301

Adarme-Vega TC, Lim KY, Timmins M, Vernen F, Li Y, Schenk PM. Microalgal biofactories: a promising approach towards sustainable omega-3 fatty acid production. Microb. Cell Fact. 2012; 72: 312-319. DOI: 10.1186/1475-2859-11-96

Simopoulos AP. The importance of the ratio of omega-6/omega-3 essential fatty acids. Biomed Pharmacother. 2002; 5. 365–379. DOI: 10.1016/S0753-3322(02)00253-6

Emeish S. Production of natural β-Carotene from Dunaliella living in the dead sea. Jordan J. Earth Environ. Sci. 2012; 4: 23-27.

Jaime L, Mendiola JA, Ibanez E, Martin-Alvarez PJ, Cifuentes A, Reglero G, Senorans FJ. β-Carotene isomer composition of sub and supercritical carbon dioxide extracts. Antioxidant Activity Measurement. J. Agric. Food Chem. 2007; 55: 10585-10590. https://doi.org/10.1021/jf0711789

Shahidi F, Wanasundara U. Omega-3 fatty acid concentrates: nutritional aspects and production technologies. Trends in Food Sci. & Technol. 1998; 9: 230-240. https://doi.org/10.1016/S0924-2244(98)00044-2

Spolaore P, Joannis-Cassan C, Duran E, Isambert A. Commercial applications of microalgae. J. Biosci. Bioeng. 2006; 101: 87-96. https://doi.org/10.1263/jbb.101.87

Senanayake SPJN, Shahidi F. Concentration of docosahexaenoic acid (DHA) from algal oil via urea complexation. J Food Lipids. 2000; 7: 51–61. DOI: 10.1111/j.1745-4522.2000.tb00160.x

Mishira VK, Temelli F, Ooraikul B. Extraction and purification of omega-3 fatty acids with an emphasis on supercritical fluid extraction. Food Res Int. 1993; 2:, 217–226. https://doi.org/10.1016/0963-9969(93)90056-O

Yamagouchi K, Murakami W, Nakano H, Konosu S, Kokura T, Yamamoto H, Kosaka M, Hata K. Supercritical carbon dioxide extraction of oils from Antarctic krill. J Agric Food Chem. 1986; 34: 904–907. https://doi.org/10.1021/jf00071a034

Hayashi K, Kishimura H. Preparation of n-3 PUFA ethyl ester concentrates from fish oil by column chromatography on silicic acid. Nippon Suisan Gakkaishi 1993; 59: 1429.

Wanasundara UN. Marine oils: stabilization, structural characterization and omega-3 fatty acid concentration’, PhD thesis, St. John’s, NF, Canada, Memorial University of Newfoundland 1996.

Hwang LS, Liang J. Fractionation of urea-pretreated squid visceral oil ethyl esters. J Am Oil Chem Soc. 2001; 78: 473–476. https://doi.org/10.1007/s11746-001-0288-x

Vonshak A. Spirulina platensis arthrospira: Physiology, Cell-Biology and Biotechnology: Taylor & Francis. 1997. https://doi.org/10.1023/A:1007911009912

Medina R, Giménez AG, Camacho FG, Perez JAS, Grima EM, Gomez AC. Concentration and purification of stearidonic, eicosapentaenoic, and docosahexaenoic acids from cod liver oil and the marine microalga Isochrysis galbana. J. Am. Oil. Chem. Soc. 1995; 72: 575–583. https://doi.org/10.1007/BF02638859

Arumugam M, Agarwal A, Arya MC, Ahmed Z. Influence of nitrogen sources on biomass productivity of microalgae Scenedesmus bijugatus. Bioresource Technology. 2013; 131: 246-249. https://doi.org/10.1016/j.biortech.2012.12.159

Dolatowski ZJ, Stadnik J, Stasiak D. Application of ultrasound in food technology. Acta. Scientiarum Polonorum. 2007; 6: 89-99. DOI: 10.1016/j.ultsonch.2010.11.023

Jayasooriya SD, Bhandari BR, Torley P, Darcy BR. Effect of high power ultrasound waves on properties of meat: a review. Int. J. Food Prop. 2004; 7: 301-319. https://doi.org/10.1081/JFP-120030039

Mason TJ, Riera E. Application of Ultrasound. In: Da-Wen S, editor. Emerging technologies for food processing, London, Academic Press. 2005: 323-351

Vilkhu K, Mawson R. Applications and opportunities for ultrasound assisted extraction in the food industry-A review. Innovative Food Sci. Emerg. Technol. 2008; 9: 161-169. https://doi.org/10.1016/j.ifset.2007.04.014

Bligh EG, Dyer WJ. A rapid method of total lipid extraction and purification. Canadian journal of biochemistry and physiology. 1959; 37: 911-917. https://doi.org/10.1139/o59-099

Mendes A, Lopes da Silva T, Reis A. DHA concentration and purification from the marine heterotrophic microalga Crypthecodinium cohnii CCMP 316 by winterization and urea complexation. Food Technol. Biotechnol. 2007; 45(1): 38–44.

Metsoviti MN, Papapolymerou G, Karapanagiotidis IT, Katsoulas N. Comparison of growth rate and nutrient content of five microalgae species cultivated in greenhouses. Plants (Basel). 2019; 8(8): 2-13. doi: 10.3390/plants8080279

. Paes CRPS, Faria GR, Tinoco NA, Castro DJFA, Barbarino E, Lourenço SO. Growth, nutrient uptake and chemical composition of Chlorella sp. and Nannochloropsis oculata under nitrogen starvation. Lat. Am. J. Aquat. Res. 2016; 44(2): 275-292. DOI: 10.3856/vol44-issue2-fulltext-9

Lopez R, Haslam N, Napier R, Sayanova O. Successful high-level accumulation of fish oil omega-3 long-chain polyunsaturated fatty acids in a transgenic oilseed crop. The Plant J.: for cell and molecular biology. 2014; 77(2): 198–208. doi: 10.1111/tpj.12378.

Wanasundara U, Shahidi F. Concentration of omega-3 polyunsaturated fatty acids of seal blubber oil by urea complexation: Optimization of reaction conditions. Food Chem. 1999; 65: 41–49. https://doi.org/10.1016/S0308-8146(98)00153-8

Ganga A, Nieto S, Sanhuez J, Romo C, Speisky H, Valenzuela A. Concentration and stabilization of n-3 polyunsaturated fatty acids from sardine oil. J. Am. Oil Chem. Soc. 1998; 75: 733–736. https://doi.org/10.1007/s11746-998-0215-4

  • چکیده مشاهده شده: 1334 بار
  • PDF (English) دانلود شده: 926 بار

آمار دانلود

##plugins.block.developedBy.blockTitle##

سامانه مجله باز

زبان

اطلاعات