Antibacterial Effect of Teucrium polium Essential Oil and Lactobacillus casei Probiotic on Escherichia coli O157:H7 in Kishk
Applied Food Biotechnology,
Vol. 5 No. 3 (2018),
5 July 2018
,
Page 131-140
https://doi.org/10.22037/afb.v5i3.19166
Abstract
Background and Objective: In recent years, there has been a rapid development in prevention of bacterial growth and spoilage in foods by the application of preservatives. Essential oils, which are known to be harmless for human, have shown positive health effects in addition to their preservative role. Likewise, probiotics exert improvement in both food product and health level of consumers. With reference to these it is important to do more research on various effects of endemic essential oils on traditional food products. A survey was carried on effect of essential oil and combination of essential oil and probiotics on growth of Escherchia coli O157:H7.
Material and Methods: Kishk samples treated with Teucrium polium essential oil (75 and 150 mg l-1) and Lactobacillus casei (108 -109 CFU ml-1) were stored up to 20 days at 4°C and the effect of Teucrium polium essential oils and probiotic fermentation on Escherchia coli O157:H7 counts were investigated.
Results and Conclusion: Teucrium polium essential oils and probiotic fermentation reduced the number of Escherichia coli during the cold storage time. The combination of Teucrium polium essential oil and probiotics showed stronger effect rather than individually added. Initial count of inoculated Escherchia coli was 6.39 log CFU g-1. Essential oil compounds of Teucrium polium included Spathulenol, Epizonaren and Bicyclo hept-3-en-2-ol in 18.39, 9.62 and 6.76%, respectively. After 20 days storage at 4°C, the number of Escherchia coli in probiotic, combination of probiotic and essential oil (150 mg l-1) and control samples decreased from 6.39 log CFU g-1 to 4.95, 4.30 and 5.8 log CFU g-1, respectively. An increase in acidity and decrease in pH were observed for all samples (p≤0.05). Based on organoleptic tests, the most accepted sample was included 75 ppm of essential oil and probiotic. According to the antibacterial effect of essential oils extracted from plant and probiotic fermentation, it was predictable that they could decline the Escherchia coli count especially in fermented dairy products. Also, due to its probiotic nature, Kishk would bring numerous health advantageous for consumer.
- ▪ Antibacterial effect ▪ Essential oil ▪ Kishk ▪ Probiotic ▪ Teucrium polium
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References
Shiroodi SG, Mohammadifar MA, Gorji EG, Ezzatpanah H, Zohouri N. Influence of gum tragacanth on the physicochemical and rheological properties of kashk. Journal of Dairy Research. 2012;79(01):93-101.
Tamime A, Muir D, Barclay M, Khaskheli M, McNulty D. Laboratory-made Kishk from wheat, oat and barley: 1. Production and comparison of chemical and nutritional composition of Burghol. Food research international. 1997;30(5):311-7.
Shakeri M-S, Shahidi F, Beiraghi-Toosi S, Bahrami A. Antimicrobial activity of Zataria multiflora Boiss. essential oil incorporated with whey protein based films on pathogenic and probiotic bacteria. International Journal of Food Science & Technology. 2011;46(3):549-54.
Ehsani ALI, Mahmoudi R. Effects of Mentha longifolia L. essential oil and Lactobacillus casei on the organoleptic properties and on the growth of Staphylococcus aureus and Listeria monocytogenes during manufacturing, ripening and storage of Iranian white-brined cheese. International Journal of Dairy Technology. 2013;66(1):70-6.
Parke DV, Lewis DFV. Safety aspects of food preservatives. Food Additives & Contaminants. 1992;9(5):561-77.
Izzo AA, Hoon-Kim S, Radhakrishnan R, Williamson EM. A Critical Approach to Evaluating Clinical Efficacy, Adverse Events and Drug Interactions of Herbal Remedies. Phytotherapy Research. 2016;30(5):691-700.
Calo JR, Crandall PG, O'Bryan CA, Ricke SC. Essential oils as antimicrobials in food systems – A review. Food Control. 2015;54(Supplement C):111-9.
Sabet Z, Roghani M, Najafi M, Maghsoudi Z. Antidiabetic effect of Teucrium polium aqueous extract in multiple low-dose streptozotocin-induced model of type 1 diabetes in rat. Journal of Basic and Clinical Pathophysiology. 2013;1(2):34-8.
Bahramikia S, Yazdanparast R. Phytochemistry and medicinal properties of Teucrium polium L.(Lamiaceae). Phytotherapy Research. 2012;26(11):1581-93.
Homayoni Rad A, Mehrabany EV, Alipoor B, Mehrabany LV, Javadi M. Do probiotics act more efficiently in foods than in supplements? Nutrition (Burbank, Los Angeles County, Calif). 2012;28(7-8):733-6. Epub 2012/06/19.
Homayouni A, Bastani P, Ziyadi S, Mohammad-Alizadeh-Charandabi S, Ghalibaf M, Mortazavian AM, et al. Effects of probiotics on the recurrence of bacterial vaginosis: a review. Journal of lower genital tract disease. 2014;18(1):79-86. Epub 2013/12/05.
Rasko DA, Webster DR, Sahl JW, Bashir A, Boisen N, Scheutz F, et al. Origins of the E. coli strain causing an outbreak of hemolytic–uremic syndrome in Germany. New England Journal of Medicine. 2011;365(8):709-17.
Grad YH, Lipsitch M, Feldgarden M, Arachchi HM, Cerqueira GC, FitzGerald M, et al. Genomic epidemiology of the Escherichia coli O104: H4 outbreaks in Europe, 2011. Proceedings of the National Academy of Sciences. 2012;109(8):3065-70.
Alipour B, Homayouni-Rad A, Vaghef-Mehrabany E, Sharif SK, Vaghef-Mehrabany L, Asghari-Jafarabadi M, et al. Effects of Lactobacillus casei supplementation on disease activity and inflammatory cytokines in rheumatoid arthritis patients: a randomized double-blind clinical trial. International journal of rheumatic diseases. 2014;17(5):519-27. Epub 2014/03/29.
Sciuchetti L, Mingis N. Ltoydia 28 (1965) 230. 4 European Pharmacopoeia, part II. Maisonneuve SA, Sainte-Ruffine. 1983:246.
Mahmoudi R, Zare P, Nosratpour S, Mardani K, Safari A. HYGIENIC EFFECTS OF TEUCRIUM POLIUM ESSENTIAL OIL AGAINST SALMONELLA TYPHIMORIUM LT2 IN PROBIOTIC YOGHURT. URMIA MEDICAL JOURNAL. 2014;25(8):769-77.
PHILLIP SM, KAILASAPATHY K, TRAN L. Viability of commercial probiotic cultures (L. acidophillus, Bifidobacterium spp., L. casei, L. paracasei and L. rhamnosus) in cheddar cheese. Int J Food Microbiol. 2006;108:276-80.
Ngwa G, Schop R, Weir S, León-Velarde C, Odumeru J. Detection and enumeration of E. coli O157: H7 in water samples by culture and molecular methods. Journal of microbiological methods. 2013;92(2):164-72.
Mahmoudi R, Nosratpour S. Teucrium polium L. essential oil: phytochemiacl component and antioxidant properties. International Food Research Journal. 2013;20(4).
Kovacevic NN, Lakusic BS, Ristic MS. Composition of the essential oils of seven Teucrium species from Serbia and Montenegro. Journal of Essential Oil Research. 2001;13(3):163-5.
Sabzeghabaie A, Asgarpanah J. Essential oil composition of Teucrium polium L. fruits. Journal of Essential Oil Research. 2016;28(1):77-80.
Mahmoudi R, Zare P, Hassanzadeh P, Nosratpour S. Effect of Teucrium polium Essential Oil on the Physicochemical and Sensory Properties of Probiotic Yoghurt. Journal of Food Processing and Preservation. 2014;38(3):880-8.
Simsek B, Sagdic O, Ozcelik S. Survival of Escherichia coli O157:H7 during the storage of Ayran produced with different spices. Journal of Food Engineering. 2007;78(2):676-80.
Lemay M-J, Choquette J, Delaquis PJ, Gariépy C, Rodrigue N, Saucier L. Antimicrobial effect of natural preservatives in a cooked and acidified chicken meat model. International Journal of Food Microbiology. 2002;78(3):217-26.
Sarabi-Jamab M, Niazmand R. Effect of essential oil of Mentha piperita and Ziziphora clinopodioides on Lactobacillus acidophilus activity as bioyoghurt starter culture. Am Eur J Agric Environ Sci. 2009;6(2):129-31.
Group JFWW. Guidelines for the evaluation of probiotics in food. London: World Health Organization, ON, Canada: Food and Agriculture Organization. 2002.
Bassolé IHN, Juliani HR. Essential oils in combination and their antimicrobial properties. Molecules. 2012;17(4):3989-4006.
Singh G, Kapoor IPS, Singh P. Effect of volatile oil and oleoresin of anise on the shelf life of yogurt*. Journal of Food Processing and Preservation. 2011;35(6):778-83.
Dehkordi FS, Yazdani F, Mozafari J, Valizadeh Y. Virulence factors, serogroups and antimicrobial resistance properties of Escherichia coli strains in fermented dairy products. BMC research notes. 2014;7(1):217.
Osaili TM, Taani M, Al‐Nabulsi AA, Attlee A, Odeh ReA, Holley RA, et al. Survival of Escherichia coli O157: H7 during the manufacture and storage of fruit yogurt. Journal of Food Safety. 2013;33(3):282-90.
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