Preparation and Characterizations of Chitosan/Citral Nanoemulsions and their Antimicrobial Activity
التكنولوجيا الحيوية الغذائية التطبيقية,
مجلد 5 عدد 2 (2018),
23 آذار 2018
,
الصفحة 69-78
https://doi.org/10.22037/afb.v5i2.19005
الملخص
Background and Objective: The antimicrobial activity of essential oils has been long recognized, however, they easily evaporate and/or decompose during preparation, owing to direct exposure to heat, pressure and light. The current study deals with the formulation and characterization of bio-based oil in water nanoemulsions and their antimicrobial activity against plant pathogens.
Material and Methods: Citral oil and low molecular weight chitosan were used for preparation of nanoemulsions in the presence of sodium tripolyphosphate. Nanoemulsions were prepared by adding dropwise citral at different ratios into an aqueous solution containing chitosan, sodium tripolyphosphate and surfactant with continuous stirring and then ultrasonication. The success of formulation was confirmed by dynamic light scattering and scanning electron microscopy techniques. Physical stability and viscosity were investigated in details. The antimicrobial activity was evaluated against Erwinia carotovora, Aspergillus niger and Rhizopus stolonifer.
Results and Conclusion: The nanoemulsions had a polydispersity index ranged from 0.508 to 0.614 and particle size from 27 to 1283 nm. The highest antimicrobial activity was observed with F1 formulation (EC50 = 23, 278 and 221 mg L-1, against Erwinia carotovora, Aspergillus niger and Rhizopus stolonifer, respectively). Based on the antimicrobial activity, the prepared chitosan/citral nanoemulsions can be a cost-effective way to protect crops from microbial pathogens. Because such formulations contain bioactive products, the development of resistant pathogens can be delayed.
Conflict of Interest: The authors declare no conflict of interest.
- ▪ Citral ▪ Chitosan ▪ Nanoemulsion ▪ Ultrasonication ▪ Characterizations ▪ Antimicrobial activity
كيفية الاقتباس
المراجع
Oussalah, M., Caillet, S. and Lacroix, M. (2006) J. Food Prot., 69: 1046-1055.
Solórzano-Santos, F. and Miranda-Novales, M.G. (2012) Curr. Opin. Biotechnol., 23: 136-141.
Gouvea, F.d.S., Rosenthal, A. and Ferreira, E.H.d.R. (2017) Ciência Rural, 47:
Aumeeruddy-Elalfi, Z., Gurib-Fakim, A. and Mahomoodally, F. (2015) Industrial Crops and Products, 71: 197-204.
Nielsen, C.K., Kjems, J., Mygind, T., Snabe, T., Schwarz, K., Serfert, Y. and Meyer, R.L. (2017) International Journal of Food Microbiology, 242: 7-12.
Sugumar, S., Nirmala, J., Ghosh, V., Anjali, H., Mukherjee, A. and Chandrasekaran, N. (2013) Journal of Basic Microbiology, 53: 677-685.
Donsì, F., Annunziata, M., Sessa, M. and Ferrari, G. (2011) LWT-Food Science and Technology, 44: 1908-1914.
Qian, C. and McClements, D.J. (2011) Food Hydrocolloids, 25: 1000-1008.
Ziani, K., Chang, Y., McLandsborough, L. and McClements, D.J. (2011) J. Agric. Food. Chem., 59: 6247-6255.
Balaure, P.C., Gudovan, D. and Gudovan, I. (2017) New Pesticides and Soil Sensors, 129.
Solans, C., Izquierdo, P., Nolla, J., Azemar, N. and Garcia-Celma, M. (2005) Current Opinion in Colloid and Interface Science, 10: 102-110.
Ghosh, V., Mukherjee, A. and Chandrasekaran, N. (2013) Ultrason. Sonochem., 20: 338-344.
Manchun, S., Dass, C.R. and Sriamornsak, P. (2014) Carbohydr. Polym., 101: 650-655.
Liang, R., Xu, S., Shoemaker, C.F., Li, Y., Zhong, F. and Huang, Q. (2012) J. Agric. Food. Chem., 60: 7548-7555.
Zhang, Z., Vriesekoop, F., Yuan, Q. and Liang, H. (2014) Food Chem., 150: 307-312.
Schieberle, P. and Grosch, W. (1988) J. Agric. Food. Chem., 36: 797-800.
Tadros, T., Izquierdo, P., Esquena, J. and Solans, C. (2004) Adv. Colloid Interface Sci., 108: 303-318.
El-Mohamedy, R., El-Gamal, N.G. and Bakeer, A.R.T. (2015) Int. J. Curr. Microbiol. Appl. Sci, 4: 629-643.
Yang, S.-J., Lin, F.-H., Tsai, H.-M., Lin, C.-F., Chin, H.-C., Wong, J.-M. and Shieh, M.-J. (2011) Biomaterials, 32: 2174-2182.
Shafiq, S. and Shakeel, F. (2010) Clinical Research and Regulatory Affairs, 27: 7-12.
Kadhim, D.H. and Abbas, H.A. (2015) International Journal of Pharmacy and Pharmaceutical Sciences, 7: 209-216.
Badawy, M.E., Rabea, E.I., Taktak, N.E. and El-Nouby, M.A. (2016) Scientifica, 2016:
Badawy, M.E., Rabea, E.I. and Taktak, N.E. (2014) Carbohydr. Polym., 111: 670-682.
Finney, D.J. (1971) Probit Analysis: 3d Ed. Cambridge University Press
Akoh, C.C. (2017) Food lipids: chemistry, nutrition, and biotechnology. CRC press
Acosta, E. (2009) Current opinion in colloid & interface science, 14: 3-15.
Shakeel, F., Ramadan, W. and Ahmed, M.A. (2009) Journal of Drug Targeting, 17: 435-441.
McClements, D.J. (2015) Food emulsions: principles, practices, and techniques. CRC press
Pal, R. (2011) Current Opinion in Colloid & Interface Science, 16: 41-60.
Bilia, A.R., Guccione, C., Isacchi, B., Righeschi, C., Firenzuoli, F. and Bergonzi, M.C. (2014) Evidence-Based Complementary and Alternative Medicine, 2014:
Guerra-Rosas, M., Morales-Castro, J., Cubero-Márquez, M., Salvia-Trujillo, L. and Martín-Belloso, O. (2017) Food Control, 77: 131-138.
São Pedro, A., Santo, I., Silva, C., Detoni, C. and Albuquerque, E. (2013) Microbial Pathogens and Strategies for Combating Them (Méndez-Vilas, A., ed.) Formatex Research Center Pulisher, 2: 1364-1374.
Buranasuksombat, U., Kwon, Y.J., Turner, M. and Bhandari, B. (2011) Food Science and Biotechnology, 20: 793-800.
Bhargava, K., Conti, D.S., da Rocha, S.R. and Zhang, Y. (2015) Food Microbiology, 47: 69-73.
Lambert, R., Skandamis, P.N., Coote, P.J. and Nychas, G.J. (2001) Journal of Applied Microbiology, 91: 453-462.
Friedman, M., Henika, P.R., Levin, C.E. and Mandrell, R.E. (2004) J. Agric. Food. Chem., 52: 6042-6048.
Zachariah, T. and Leela, N. (2006) Handbook of herbs and spices. 1st ed. Cambridge, UK: Woodhead Publishing. p, 177-211.
Hamouda, T. and Baker, J. (2000) Journal of Applied Microbiology, 89: 397-403.
Caccioni, D.R., Guizzardi, M., Biondi, D.M., Renda, A. and Ruberto, G. (1998) International Journal of Food Microbiology, 43: 73-79.
Joe, M.M., Bradeeba, K., Parthasarathi, R., Sivakumaar, P.K., Chauhan, P.S., Tipayno, S., Benson, A. and Sa, T. (2012) Journal of the Taiwan Institute of Chemical Engineers, 43: 172-180.
Saddiq, A.A. and Khayyat, S.A. (2010) Pesticide Biochemistry and Physiology, 98: 89-93.
Luo, M., Jiang, L. and Zou, G. (2001) Chinese Journal of Biochemistry and Molecular Biology, 18: 227-232.
Nychas, G., Skandamis, P., Tassou, C. and Roller, S. (2003) Natural Antimicrobials for The Minimal Processing of Foods, 176-200.
- الملخص المشاهدات: 2456 الأوقات
- PDF (English) التنزيلات: 1562 الأوقات