Synergistic Effect of Elicitors in Enhancement of Ganoderic Acid Production: Optimization and Gene Expression Studies
Applied Food Biotechnology,
Vol. 2 No. 3 (2015),
30 June 2015
,
Page 57-62
https://doi.org/10.22037/afb.v2i3.8715
Abstract
Ganoderma lucidum is one of the most well-known fungi, and has many applications in medicine. Ganoderic acid is among the valuable secondary metabolites of Ganoderma lucidum, and responsible for the inhibition of the tumor cell growth and cancer treatment. Application of ganoderic acid has been limited because of low yields of its production from Ganoderma lucidum. The present study aims to investigate the synergistic effect of elicitors including methyl jasmonate and aspirin on the production of ganoderic acid derived from Ganoderma lucidum mushroom in a shaken flasks using response surface methodology. The results showed that the optimal dose of methyl jasmonate and asprin significantly impacts on the amount of ganoderic acid production as a response (p<0.05). The proposed model predicted the maximum ganoderic acid production as 0.085 mgml-1 in which the optimal concentrations obtained for methyl jasmonate and asprin were 250 mM and 4.4 mM, respectively. Also the influence of ganoderic acid production on the expression of 3-hydroxy-3-methyl-glutaryl coenzyme A reductase and squalene synthase (two important metabolic pathway genes in ganoderic acid) was investigated, and the results showed that these genes’ expression has increased by 10 and 11 folds, respectively.
- Cancer
- Elicitor
- Ganoderic acid
- Ganoderma lucidum
- Gene expression
How to Cite
References
Smith JE, Rowan NJ, Sullivan R. Medicinal mushroom: a rapidly developing area of biotechnology for cancer therapy and other bioactivities. Biotechnol Lett. 2002; 24: 1839-1845.
Komoda Y, Shimizu M, Sonoda Y, Sato Y. Ganoderic acid and its derivatives as cholesterol synthesis inhibitors. Chem Pharm Bull. 1989; 37: 531-535.
Wu GS, Lu JJ, Guo JJ, Li YB, Tan W, Dang YY, Zhong ZF, Xu ZT, Chen XP, Wang YT. Ganoderic acid DM a natural triterpenoid induces DNA damage G1 cell cycle arrest and apoptosis in human breast cancer cells. Fitoterapia 2012; 83: 408-414.
Johnson BM, Doonan BP, Radwan FF, Haque A. Ganoderic acid DM an alternative agent for the treatment of advanced prostate cancer. Open Prost Cancer J. 2010; 3: 78-85.
Gao JJ, Min BS, Ahn EM, Nakamura N, Lee HK, Hattori M. New triterpene aldehydes, from Ganoderma lucidum and their cytotoxicity against murine and human tumor cells. Chem Pharm Bull. 2002; 50: 837-840.
Li YQ, Wang SF. Anti-hepatitis B activities of ganoderic acid from Ganoderma lucidum. Biotechnol Lett. 2006; 28: 837-841.
Mekkawy SE, Meselhy MR, Nakamura N, Tezuka Y, Hattori M, Kakiuchi N, Shimotohno K, Otake T. Anti HIV-1 and Anti HIV-1 protease substances from Ganoderma lucidum. Phytochem. 1998; 49: 1651-1657.
Min BS, Nakamura N, Miashiro H, Bae KW, Hattori M. Triterpenes from the spores of Ganoderma lucidum and their inhibitory activity against HIV-1 protease. Chem Pharm Bull. 1998; 46: 1607-1612.
Guest E. Ganoderma lucidum in cancer research. Leukemia Res. 2006; 30: 767-768.
Sonadiya BS, Thakur GS, Baghel RK, Prasad GBKS, Bisen PS. Ganoderma lucidum: A potent pharma-cological macrofungus. Curr Pharm Biotechnol. 2009; 10: 717- 742.
Shi L, Ren A, Mu D, Zhao M. Current progress in the study on biosynthesis and regulation of ganoderic acids. Appl Microbiol Biotechnol. 2010; 88: 1243-1251.
Liang CX, Li YB, Xu JW, Wang JL, Miao XL, Tang YJ, Gu T, Zhong JJ. Enhanced biosynthetic gene expressions of ganoderic acids in static liquid culture of Ganoderma lucidum under Phenobarbital induction. Appl Microbiol Biotechnol. 2010; 86: 1367-1374.
Liu GQ, Xiao HX, Wang XL, Zhao Y, Zhang YG, Ren GP. Stimulated production of triterpenoids of Ganoderma lucidum by an Ether extract from the medicinal insect, Catharsius molossus, and identifi-cation of the key stimulating active components. Appl Biochem Biotechnol. 2011; 165: 87-97.
Zhang WX, Tang YJ, Zhong JJ. Impact of oxygen level in gaseous phase on gene transcription and ganoderic acid biosynthesis in liquid static cultures of Ganoderma lucidum. Bioprocess Biosyst Eng. 2010; 33: 683-690.
Zhao W, Xu JW, Zhong JJ. Enhanced production of ganoderic acids in liquid culture of Ganoderma lucidum under nitrogen-limiting conditions. Bioresour Technol. 2011; 102: 8185-8190.
Zhang J, Zhong JJ, Genga A. Improvement of ganoderic acid production by fermentation of Ganod-erma lucidum with cellulase as an elicitor. Process Biochem. 2014; 49: 1580-1586.
Zhu LW, Zhong JJ, Tang YJ. Significance of fungal elicitors on the production of ganoderic acid and Ganoderma polysaccharides by the submerged culture of medicinal mushroom Ganoderma lucidum. Process Biochem. 2008; 43: 1359-1370.
Ren A, Qin L, Shi L, Dong X, Mu DSh, Li YX, Zhao MW. Methyl jasmonate induces ganoderic acid biosynthesis in the basidiomycetous fungus Ganod-erma lucidum. Bioresour Technol. 2010; 101: 6785-6790.
You BJ, Lee MH, Tien N, Lee MS, Hsieh HC, Tseng LH, Chung YL, Lee HZ. A novel approach to enhancing ganoderic acid production by Ganoderma lucidum using apoptosis induction. PLoS ONE. 2013; 8: 1-7.
Montgomery DC. Design and Analysis of Experiments (6th Edition). John Wiley & Sons, New York, 2006.
Parvin N, Doryab A. The application of response surface methodology for the optimization of compact-ion parameters in Cu-Al2O3 composite. J Powder Metall Min. 2013; 2:108-110.
Fang QH, Zhong JJ. Two-stage culture process for improved production of ganoderic acid by liquid fermentation of higher fungus Ganoderma lucidum. Biotechnol Prog. 2002; 18:51-54.
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