Optimization of Monacolin Production in a Controlled System
Applied Food Biotechnology,
Vol. 2 No. 4 (2015),
AbstractMonascus purpureus is a fungus that had been cultured on the rice in eastern Asian countries since thousands years ago and used as a food for long years. The fungus produces very valuable metabolites with polyketide structure. The most important metabolite is Monacolin K, lovastatin or competitive inhibitor of 3-Hydroxy-3-Methylglutaryl-Coenzyme A reductase (an affective enzyme in cholesterol synthesis). This metabolite has various properties including reducing blood cholesterol, preventing infection, and treatment of progressive renal disease, a variety of tumors, vascular diseases and bone fractures. In this study, Monascus purpureus PTCC5303 has been used for lovastatin production in liquid fermentation. The nutritional concentrations that were significant in biomass and lovastatin production including maltose and MgSO4 were optimized by Response Surface Methodology in a mili-bioreactor. The optimum concentration of maltose and MgSO4 was obtained as 10 g l-1 and 0.78 g l-1, respectively. According to our results, maximum lovastatin production under optimum conditions including maltose 10 g l-1, peptone 5 g l-1, MgSO4.7H2O 0.78 g l-1, MnSO4.H2O 0.5 gl-1, KH2PO4 4 g l-1, thiamine 0.1 g l-1, and pH=7 at 30 °C, 130 rpm and flow rate 1.8 l min-1 was obtained to be 309 μg l-1 after 10 days of fermentation period.
- Monascus purpureus
- Oxygen Transfer Rate
How to Cite
Nijjar PS, Burke FM, Bloesch A, Rader DJ. Role of dietary supplements in lowering low-density lipoprotein cholesterol: a review. J Clin Lipidol. 2010; 4: 248-258.
Pattanagul P, Pinthong R, Phianmongkhol, Leksawasdi
N. Review of angkak production (Monascus purpureus). Chiang Mai J Sci. 2007; 34(3): 319-328.
Ajdari Z, Ebrahimpour A, Abdul Manan M, Hamid M, Mohamad R, Ariff AB. Assessment of monacolin in the fermented products using Monascus purpureus FTC5391. J Biolmed Biotechnol. 2011; 2011: 1-9.
Chen ZY, Ma KY, Liang Y, Peng C, Zuo Y. Role and classification of cholesterol-lowering functional foods. J Funct Foods. 2011; 3: 229-254.
Singer II, Scott S, Kazazis DM, Huff JW. Lovastatin, an inhibitor of cholesterol synthesis, induces hydroxyl- methylglutaryl-coenzyme a reductase directly on membr- anes of expanded smooth endoplasmic reticulum in rat hepatocytes. Proc Natl Acad Sci. 1988; 85: 5264-5268.
Endo A, Monacolin K. A new hypocholesterolemic agent that specifically inhibits 3-hydroxy-3-methylglutaryl coenzyme A reductase. J Antibiotics. 1980; 33: 334-336.
Alberts A, Chen J, Kuron G, Hunt V, Huff J, Hoffman C, Rothrock J, Lopez M, Joshua H, Harris E. Mevinolin: a highly potent competitive inhibitor of hydroxymethyl glutaryl-coenzyme A reductase and a cholesterol- lowering agent. Proc Natl Acad Sci. 1980; 77: 3957- 3961.
Hajjaj H, Niederberger P, Duboc P. Lovastatin biosynthesis by Aspergillus terreus in a chemically defined medium. Appl Environ Microbiol. 2001; 67: 2596-2602.
Manzoni M, Rollini M. Biosynthesis and biotechnological production of statins by filamentous fungi and application of these cholesterol-lowering drugs. Appl Microbiol Biotechnol. 2002; 58: 555-564.
Zhou Q, Liao JK. Statins and cardiovascular diseases: from cholesterol lowering to pleiotropy. Current Pharm Des. 2009; 15: 467-478.
Kumari HP M, Dhale MA, Govindaswamy V. Optimization of monacolin K production by Monascus purpureus MTTC 410 in submerged fermentation. Int J Food Eng.. 2012; 8: 321-328.
Patakova P. Monascus secondary metabolites: production and biological activity. J Ind Microbiol Biotechnol. 2013; 40: 169-181.
Srianta I, Ristiarini S, Nugerahani I, Sen SK, Zhang BB, Xu GR, Blanc PJ. Recent research and development of Monascus fermentation products. Int Food Res J. 2014; 21(1): 1-12.
Sayyad SA, Panda BP, Javed S, Ali M. Optimization of nutrient parameters for lovastatin production by Monascus purpureus MTCC 369 under submerged fermentation using response surface methodology. Appl Microbial Biotechnol. 2007; 73: 1054-1058.
Pawlak M, Bizukojć M, Ledakowicz S. Impact of bioreactor scale on lovastatin biosynthesis by Aspergillus terreus ATCC 20542 in a batch culture. Chem Process Eng. 2012; 33: 71-84.
Seraman S, Rajendran A, Thangavelu V. Statistical optimization of anticholesterolemic drug lovastatin production by the red mold Monascus purpureus. Food Bioprod Process. 2010; 88: 266-276.
Radha K, Lakshmanan D. A review: lovastatin production and applications. Asian J Pharm Clin Res. 2013; 6: 223-228.
Kumar MS, Jana SK, Senthil V, Shashanka V, Kumar SV, Sadhukhan A. Repeated fed-batch process for improving lovastatin production. Proc Biochem. 2000; 36: 363-368.
Maniyom S, Markx GH. Biomass and pigment production by Monascus during miniaturized submerged. culture on Adlay. World Acad. Sci. Eng Technol 2012; 68: 1786-1791.
Chang CY, Lee CL, Pan TM. Statistical optimization of medium components for the production of Antrodia cinnamomea AC0623 in submerged cultures. Appl Microbiol Biotechnol. 2006; 72: 654-661.
Masurekar PS. Nutritional and engineering aspects of microbial process development. In: Peterson F, Amstutz
R. Natural compounds as drugs. Volume I, Progress in drug research. Springer, Switzerland, 2008, 291-328.
Su YC, Wang JJ, Lin TT, Pan TM. Production of the secondary metabolites γ-aminobutyric acid and monacolin K by Monascus. J Ind Microbiol Biotechnol. 2003; 30: 41-46.
Mansoori S, Ashrafi SJ, Yazdian F. Optimization of lovastatin production in submerged fermentation by Monascus purpureus. Tarbiat Modares J. 2014; 5(2): 70- 80.
Pansuriya RC, Singhal RS. Response surface method- ology for optimization of production of lovastatin by solid state fermentation. Braz J Microbiol. 2010; 41: 164- 172.
Sun JL, Zou X, Liu AY, Xiao TF. Elevated yield of Monacolin K in Monascus purpureus by fungal elicitor and mutagenesis of UV and LiCl. Biol Res. 2011; 44: 377-382.
Buckland B, Gbewonyo K, Hallada T, Kaplan L, Masurekar P. Production of lovastatin, an inhibitor of cholesterol accumulation in humans. In: Demain AL, Somkuti GA, Hunter-Cevera JC, Rossmoore HW. Novel microbial products for medicine and agriculture. Elsevier, Amsterdam. 1989; 161-169.
- Abstract Viewed: 7206 times
- PDF Downloaded: 630 times