Optimal Medium Composition to Enhance Poly-β-hydroxybutyrate Production by Ralstonia eutropha Using Cane Molasses as Sole Carbon Source
Applied Food Biotechnology,
Vol. 2 No. 3 (2015),
30 June 2015
,
Page 39-47
https://doi.org/10.22037/afb.v2i3.8883
Abstract
In order to reduce the costs associated with poly-β-hydroxybutyrate production, growth and poly-β-hydroxybutyrate production of Ralstonia eutropha were studied in batch culture on different carbon sources. Experiments were designed and conducted to not only lower the cost of poly-β-hydroxybutyrate production by using inexpensive substrates, but also to increase poly-β-hydroxybutyrate production by optimizing the culture medium composition. Low cost, abundant carbon sources, including cane molasses, beet molasses, soya bean, and corn steep liquor were used to investigate the possibility of poly-β-hydroxybutyrate production in such renewable carbon sources. Based on the experimental results, cane molasses with production of 0.49 gL-1 poly-β-hydroxybutyrate was selected as the most efficient carbon source. To improve bacterial growth and poly-β-hydroxybutyrate production, different chemicals were then used to pretreat the cane molasses. Sulfuric acid, with 33% enhancement in poly-β-hydroxybutyrate production, revealed the highest efficiency in removing heavy metals and suspended impurities and was used to pretreat cane molasses in the subsequent experiments. Additionally, to make the process even more efficient and ultimately more effective, urea and corn steep liquor were used as nitrogen/minerals and vitamin sources, respectively. Using the response surface methodology and through a 2n factorial central composite design, the medium composition was then optimized, and maximum biomass concentration of 5.03 gL-1 and poly-β-hydroxybutyrate concentration of 1.63 gL-1 were obtained.
- Cane molasses
- Optimization
- Polyhydroxybutyrate
- Ralstonia eutropha
- Response Surface Methodology
How to Cite
References
Zheng Y, Yanful EK, Bassi AS. A review of plastic waste biodegradation. Crit Rev Biotechnol. 2005; 25: 243-250.
Barnes DKA, Galgani F, Thompson RC, Barlaz M. Accumulation and fragmentation of plastic debris in global environments. Phil Trans R Soc B. 2009; 364: 1985-1998.
Howdeshell KL, Hotchkiss AK, Thayer KA, Vandenbergh JG, Vom Saal FS. Exposure to bisphenol A. advances puberty. Nature. 1999; 401: 763-764.
Chandra R, Rustgi R. Biodegradable polymers. Prog Polym Sci. 1998; 23: 1273-1335.
Suriyamongkol P, Weselake R, Narine S, Moloney M, Shah S. Biotechnological approaches for the production of polyhydroxyalkanoates in microorganisms and plants– A review. Biotechnol Adv. 2007; 25: 148-175.
Langevelda, JWA, Dixonb J, Jaworskic JF. Development perspectives of the biobased economy: A review. Crop Sci. 2010; 50: 142-151.
Clark JH, Luque R, Matharu AS. Green chemistry, biofuels, and biorefinery. Annu Rev Chem Biomol Eng. 2012; 3: 183-207.
Byrom D. Polymer synthesis by microorganisms: Technology and economics. Trends Biotechnol 1987; 5: 246-250.
Anderson AJ, Dawes EA. Occurrence, metabolism, metabolic role and industrial uses of bacterial polyhydroxyalkanoates. Microbiol Rev. 1990; 54: 450-472.
Madison LL, Huisman GW. Metabolic engineering of poly(3-hydroxyalkanoates): From DNA to plastic. Microbiol Mol Biol Rev. 1999; 63: 21-53.
Khanna S, Srivastava AK. Recent advances in microbial polyhydroxyalkanoates. Process Biochem. 2005a; 40: 607-619.
Khanna S., Srivastava AK. Statistical media optimization studies for growth and PHB production by Ralstonia eutropha, Process Biochem. 2005; 40: 2173-2182.
Lee SY. Bacterial polyhydroxyalkanoates. Biotechnol Bioeng. 1996; 49: 1-14.
Amass W, Amass A, Tighe B. A review of biodegradable polymers: Uses, current developments in the synthesis and characterization of biodegradable polymers, blends of biodegradeable polymers and recent advances in biodegradeation studies. Polym Int. 1998; 47: 89-144 .
Pietrini M, Roes L, Martin K, Patel MK, Chiellini E. Comparative life cycle studies on poly(3-hydroxybutyrate)-based composites as potential replacement for conventional petrochemical plastics Biomacromol. 2007; 8: 2210-2218.
Ibrahim MHA, Steinbuchel A. Poly (3-Hydroxybutyrate) Production from Glycerol by Zobellella denitrifican MW1 via High-Cell-Density Fed-Batch Fermentation and Simplified Solvent Extraction. Appl Environ Microbiol. 2009; 75: 6222-6231.
Khosravi-Darani K, Vasheghani-Farahani E. Microorganisms and systems for production of poly(hydroxybutyrate) as a biodegradable polymer. Iran J Chem Chem Eng. 2005; 24: 1-19.
Khosravi-Darani K, Vasheghani-Farahani E. Application of supercritical fluid extraction in biotechnology. Crit Rev Biotechnol. 2005; 25: 1–12.
Koller M. Bona R, Braunegg G, Hermann C, Horvat P, Kroutil M, Martinz J, Neto J, Pereira L, Varila P. Production of polyhydroxy-alkanoates from agricultural waste and surplus materials. Biomacromol. 2005; 6: 561-565.
Koller M, Salerno A. Sousa Dias M, Reiterer A, Braunegg G. Modern biotechnological polymer synthesis: A review. Food Technol Biotechnol. 2010; 48: 255-269.
Sathiyanarayanan G, Saibaba G, Seghal Kiran G, Selvin. A statistical approach for optimization of polyhydroxybutyrate production by marine Bacillus subtilis MSBN17. Int J Biol Macromol. 2013; 59: 170-177.
Nath A., Dixit M, Bandiya A, Chavda S, Desai AJ. Enhanced PHB production and scale up studies using cheese whey in fed batch culture of Methylobacterium sp. ZP24. Bioresour Technol. 2008; 99: 5749-5755.
Singh G, Kumari A, Mittal A, Goel V, Yadav A, Aggarwal NK. Cost effective production of poly-β-hydroxybutyrate by Bacillus subtilis NG05 using sugar industry waste water. J Polym Environ. 2013; 21: 441-449.
Khosravi-Darani K, Mokhtari ZB, Amai T, Tanaka K. Microbial production of poly(hydroxylbutyrate) from C1 carbon sources. Appl Microbiol Biotechnol. 2013; 97: 1407-1424.
Arun A, Murrugappan RM, Ravindran AD, Veeramanikandan V, Balaji S. Utilization of various industrial wastes for the production of poly-β-hydroxy butyrate by Alcaligenes eutrophus. Afr J Biotechnol. 2006; 5: 1524-27.
Kucukaşik F, Kazak H, Guney D, Finore I, Poli A, Yenigun O, Nicolaus B, Oner ET. Molasses as fermentation substrate for levan production by Halomonas sp. Appl Microbiol Biotechnol. 2011; 89: 1729 -1740.
Roukas T, Kotzekidou P. Pretreatment of date syrup to increase citric acid production. Enzyme Microb Technol. 1997; 21: 273-276.
Roukas T. Pretreatment of beet molasses to increase pullulan production. Process Biochem. 1998; 33: 805-810.
Nasir DS, Charles T, Bright Singh IS. Preliminary optimization of PHB production by Vibrio sp. MCCB 237 isolated from Marine Environment. Res J Chem Sci. 2014; 4: 10-13.
Bezerra MA, Santelli RE, Oliveira EP, Villar LS, Escaleira LA. Response surface methodology (RSM) as a tool for optimization in analytical chemistry. Talanta. 2008; 76: 965-977.
Yang YH, Brigham CJ, Budde CF, Boccazzi P, Willis LB, Hassan MA, Yusof ZA, Rha C, Sinskey AJ. Optimization of growth media components for polyhydroxyalkanoate (PHA) production from organic
acids by Ralstonia eutropha. Appl Microbiol Biotechnol 2010; 87: 2037-2045.
Reinecke F, Steinbuchel A: Ralstonia eutropha strain H16 as model organism for PHA metabolism and for biotechnological production of technically interesting biopolymers. J Mol Microbiol Biotechnol. 2009; 16: 91-108.
Kitamura S, Doi Y. Staining method of poly (3- hydroxyalkanotes acids) producing bacterial by nile blue. Biotechnol Tech. 1994; 8: 345-350.
Pinto JSS, Lanças FM. Design, Construction and evaluation of a simple pressurized solvent extraction system. J Braz Chem Soc. 2009; 20: 913-917.
Senior PJ, Beech GA, Ritchie GAF, Dawes EA: Role of oxygen limitation in formation of poly-beta-hydroxybutyrate during batch and continuous culture of Azotobacter beijerinckii. Biochem J. 1972; 128: 1193-1201.
Dekwer, D, Hempel, DC. Microaerophilic production of alginate by Azotobacter vinelandii, Von der Gemeinsamen Naturwissenscha ftlichen, Fakultat der Technischen UN. Carolo-Wilhelmina zu Braunschweig, Edited by Wael Sabra, aus Alexandria, Agypten. 1999; 37-54.
Khanafari A, Akhavansepahei A, Mogharab M. Production and recovery of poly-β-hydroxybutyrate from whey degradation by Azotobacter. Iran J Environ Health Sci Eng. 2006; 3: 193-198.
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