Phytosome and Liposome: The Beneficial Encapsulation Systems in Drug Delivery and Food Application
Applied Food Biotechnology,
Vol. 2 No. 3 (2015),
30 June 2015
AbstractDue to poor solubility in lipids, many of bioactive components (Nutraceutical materials) show less bioactivity than optimal state in water solution. Phytosomes improve absorption and bioavailability of biomaterials. Liposomes, spherical shaped nanocarriers, were discovered in the 1960s by bangham. Due to their composition, variability and structural properties, liposomes and phytosomes are extremely versatile, leading to a large number of applications including pharmaceutical, cosmetics and food industrial fields. They are advanced forms of herbal formulations containing the bioactive phytoconstituents of herb extracts such as flavonoids, glycosides and terpenoids, which have good ability to transit from a hydrophilic environment into the lipid friendly environment of the outer cell membrane. They have better bioavailability and actions than the conventional herbal extracts containing dosage. Phytosome technology has increasing effect on the bioavailability of herbal extracts including ginkgo biloba, grape seed, green tea, milk thistle, ginseng, etc., and can be developed for various therapeutic uses or dietary supplements. Liposomes are composed of bilayer membranes, which are made of lipid molecules. They form when phospholipids are dispersed in aqueous media and exposed to high shear rates by using micro-fluidization or colloid mill. The mechanism for formation of liposomes is mainly the hydrophilic-hydrophobic interactions between phospholipids and water molecules. Here, we attempt to review the features of phytosomes and liposomes as well as their preparation methods and capacity in food and drug applications. Generally, it is believed that phytosomes and liposomes are suitable delivery systems for nutraceuticals, and can be widely used in food industry.
- Bioavailability Encapsulation Liposomes Phytosomes Plant extract
How to Cite
Patella J, Patelb R, Khambholjab K, Patela N. An overview of phytosomes as an advanced herbal drug delivery system. Asian J Pharm Sci. 2009; 4(6):363-371.
Kalita B, Das M, Sharma A. Novel phytosome formulations in making herbal extracts more effective. J Pharm Technol. 2013; 6 (11):1295-1301.
Semalty A, Semalty M, Riwat MSM, Franceschi F. Supramolecular phospholipids-polyphenolics interaction: the phytosome strategy to improve the bioavailability of phytochemicals. Fitoterapia. 2010; 81 (5): 306-314.
Kidd PM, Head K. A review of the bioavailability and clinical efficacy of milk thistle phytosome: a silybin-phosphatidylcholine complex (Siliphos). Altern Med Rev. 2005; 10:193- 203.
Hoh C, Boocock D, Marczylo D, Singh R, Berry DP, Dennison AR. Pilot study of oral silibinin, a putative chemopreventive agent in colorectal cancer patients: silibinin levels in plasma, colorectum and liver and their pharmacodynamic consequences. Clin Cancer Res. 2006; 12 (9): 2944-2950.
Carini R, Comoglio A, Albano E, Poli G. Lipid peroxidation and irreversible damage in the rat hepatocyte model. Protection by the silybin-phospholipid complex IdB 1016. Biochem Pharmacol. 1992; 43: 2111-2115.
Conti M, Malandrino S, Magistretti MJ. Protective activity of silipide on liver damage in rodents. Jpn J Pharmacol. 1992; 60: 315-321.
Morazzoni P, Montalbetti A, Malandrino S, Pifferi G. Comparative pharmacokinetics of silipide and silymarin in rats. Eur J Drug Metab Pharmacokinet. 1993; 18:289-297.
Yanyu X, Yunmei S, Zhipeng C, Quineng P. The preparation of silybin-phospholipid complex and the study on its pharmacokinetics in rats. Int J Pharm. 2006; 307 (1): 77-82.
Maiti K, Mukherjee K, Gantait A, Curcumin-phospholipid complex, preparation, therapeutic evaluation and pharmacokinetic study in rats. Int J Pharm. 2007; 330 (1-2): 155-163.
Maiti K, Mukherjee K, Gantait A, Ahamed HN, Saha BP, Mukherjee PK. Enhanced therapeutic benefit of quercetin- phospholipid complex in carbon tetrachloride induced acute liver injury in rats: A comparative study. Iran J Pharmacol Ther. 2005; 4: 84-90.
Townsend PA, Scarabelli TM, Pasini E, Gitti G, Menegazzi M, Suzuki H, Knight RA, Latchman DS, Stephanou A. Epigallocatechin 3-O- gallate inhibits STAT-1 activation and protects cardiac myocytes from ischemia/reperfusion induced apoptosis. FASEB J. 2004; 18:1621-1623.
Bordoni A, Hrelia S, Angeloni C, Giordano E, Guarnieri C, Caldarera CM, Biagi PL. Green tea protection of hypoxia/reoxygenation injury in cultured cardiac cells. J Nutr Biochem. 2002; 13:103-111.
Angeloni C, Maraldi T, Ghelli A, Rugolo M, Leoncini E, Hakim G, Hrelia S. Green tea modulates alpha1adrenergic stimulated glucose transport in cultured rat cardiomy ocites. J Agric Food Chem. 2007; 55: 7553-7558.
Tedeschi E, Menegazzi M, Yao Y, Suzuki H, Förstermann U, Kleinert H. Green tea inhibits human inducible NO Synthase expression by down regulating signal transducer and activator of transcription-1a activation. Mol Pharmacol. 2004; 65: 111-120.
Gibb BF, Kermasha S, Alli L, Mulligan CN. Encapsulation in the food industry: a review. Int J Food Sci Nutr. 1999; 50(3): 213-240.
Zuidam N, Shimoni E. Overview of Microencapsulates for Use in Food Products or Processes and Methods to Make Them. In: Zuidam NJ, Nedovic, V. Encapsulation Technologies for Active Food Ingredients and Food Processing. Springer-Verlag New York 1Ed. 2010: 3-29.
Semalty A, Semalty M, Singh D, Rawat MSM. Preparation and characterization of phospholipid complexes of naringenin for effective drug delivery. J Incl Phenom Macrocycl Chem. 2010; 67: 253-260.
Sessa G, Weissmann G. Phospholipid spherules (liposomes) as a model for biological membranes. J Lipid Res. 1968; 9: 310-318.
Laouini A, Jaafar-Maalej C, Limayem-Blouza I, Sfar C, Charcosset C, Fessi H. Preparation, characterization and applications of liposomes: state of art. J Colloid Sci Biotechnol. 2012; 1 (2): 147-168.
Singh D, Rawat MSM, Semalty A, Semalty M. Chrysophanol-phospholipids complex. J Therm Anal Calorim. 2013; 111 (3): 2069-2077.
Gupta NK, Dixit VK. Bioavailability enhancement of curcumin by complexation with phosphatidyl choline. J Pharm Sci. 2011; 100: 1987-1995.
Zhang J, Gao W, Bai Sh, Chen H, Qiang Q, Liu Z. Glycyrrhizic acid-phospholipid complex: preparation process optimization and therapeutic and pharmacokinetic evaluation in rats. Am J Pharm. 2011; 30: 1621-1630.
Kusumawati I, Yusuf H. Phospholipid complex as carrier of Kaempferia galangal rhizome extract to improve its analgesic activity. Int J Pharm Pharm Sci. 2011; 3: 44-46.
Zaidi SMA, Pathan SA, Ahmad FJ, Surender S, Jamil S, Khar RK. Neurophar-macological evaluation of Paeoniaemodi root extract phospholipid complex in mice. Planta Med. 2011; 77: 123-123.
Peng Q, Gong T, Zuo J, Liu J, Zhao D, Zhang Z. Enhanced oral bioavailability of salvianolic acid B by phospholipid complex loaded nanoparticles. Pharmazie. 2008; 63: 661-666.
Qin X, Yang Y, Fan TT, Gong T, Zhang XN, Huang Y. Preparation, characterization and in vivo evaluation of bergenin-phospholipid complex. Acta Pharmacol Sin. 2010; 31: 127-136.
Sikarwar MS, Sharma S, Jain AK, Parial SD. Preparation, characterization and evaluation of Marsupsin-phospholipid complex. AAPS Pharm Sci Tech. 2008; 9: 129 -137.
Li Y, Yang DJ, Chen SL, Chen SB, Chan AS. Process parameters and morphology in puerarin, phospholipids and their complex microparticles generation by super-critical antisolvent precipitation. Int J Pharm. 2008; 359: 35-45.
Pathan R, Bhandari U. Preparation and characterization of embelin phospholipid complex as effective drug delivery tool. J Incl Phenom Macrocycl Chem. 2011; 69: 139-147.
Morazzoni P, Bombardelli E. Phospholipid complexes prepared from extracts of Vitis vinifera as anti-atherosclerotic agents. Grant, Indena Spa, Milan, Italy 2001, Patent No. US6297218 B1.
Singh D, Rawat MS, Semalty A, Semalty M. Rutin- phospholipid complex: an innovative technique in novel drug delivery system- NDDS. Curr Drug Deliv. 2012; 9: 305-314.
Awasthi R, Kulkarni JTV. Phytosomes: pawar, an approach to increase the bioavailability of plants extracts. Int J Pharm Pharm Sci. 2011; 3(2): 13.
Sindhumol PG, Thomas M, Mohanachandran PS. Phytosome: a novel dosage form for enhancement of bioavailabaility of botanical and neutraceuticals. Int J Pharm Pharm Sci. 2010; 2 (4): 10-14.
Wendel Lesithin A. Encyclopedia of Surface and Colloid Science, Volume 5. In: Kirk-Othmer encyclopedia of chemical technology. 4th Ed.; John Wiley & Sons: New York, 1995.
Hathout RM, Mansour S, Mortada ND, Guinedi AS. AAPS. Liposomes as an ocular delivery system for acetazolamide: in vitro and in vivo studies. AAPS Pharm Sci Technol. 2007; 8, 1-12.
Mohammed AR, Weston N, Coombes AGA, Fitzgerald M, Perrie Y. Liposome formulation of poorly water soluble drugs: optimisation of drug loading and ESEM analysis of stability. Int J Pharm. 2004; 285: 23-34.
Skalko-Basnet N, Pavelic Z, Becirevic-Lacan M. Liposomes containing drug and cyclodextrin prepared by the one-step spray-drying method. Drug Dev Ind Pharm. 2000; 26 (12): 1279 -1284.
Li C, Deng Y. A novel method for the preparation of liposomes: Freeze drying of monophase solutions. J Pharm Sci. 2004; 93: 1403-1414.
Kidd P. Phospholipids: Versatile nutraceuticals for functional foods. Functional Foods and Nutraceuticals. 2002. 1-5.
Amit PYST, Rakesh S, Poojan P. Phytosome: phytolipid drug delivery system for improving bioavailability of herbal drug. J Pharm Sci Biosci Res. 2013; 3(2): 51-57.
Husch J, Dutagaci B, Glaubitz C, Geppert T, Schneider G, Harms M. Structural properties of so-called NSAID-phospholipid-complexes. Eur J Pharm Sci. 2011; 44 (1-2): 103-116.
Kidd PM. Bioavailability and activity of phytosome complexes from botanical polyphenols: the silymarin, curcumin, green tea, and grape seed extracts. Altern Med Rev. 2009; 14 (3): 226-246.
Gandhi A, Dutta A, Pal A, Bakshi P. Recent trends of phytosomes for delivering herbal extract with improved bioavailability. J Pharmacogn Phytochem. 2012; 1 (4): 6-14.
Bhattacharya S. Phytosomes: the new technology for enhancement of bioavailability of botanicals and nutraceuticals. Int J Health Res. 2009; 2(3): 225-232.
Gabetta B, Zini G, Pifferi G. Spectroscopic studies on IdB 1016, a new flavanolignan complex. Planta Med. 1989; 55 (7): 615-615.
Kaur P, Sen P, Arora S, Sharma A. Emerging trends and future prospective of phytosomes as carrier for enhanced bioavailability of bioactives: A review. Ph Tech Med. 2012;1(3):109-119.
Kumar P, Yadav S, Agarwal A, Kumar N. Phytosomes a novel phyto-phospholipid carriers: an overview. Int J Pharm Res Dev. 2010; 2 (6): 1-7.
Dayan N, Touitou E. Carrier for skin delivery of trihexyphenidyl HCl: Ethosomes vs liposomes. Biomaterials. 2002; 21:1879-1885.
Facino R. M, Carini M, Aldini G, Bombardelli E, Morazzoni P, Morelli R. Free radicals scavenging action and anti-enzyme activities of procyanidines from Vitis vinifera. A mechanism for their capillary protective action. Arzneimittelforschung. 1994; 44 (5): 592-601.
Abu-Reidah I, Jamous R, Ali-Shtayeh M. Phytochemistry, pharmacological properties and industrial applications of rhus coriaria l. (Sumac). Jordan J Biol Sci. 2014; 4 (7): 233-244.
Bozkurt H. Investigation of the effect of Sumac extract and BHT addition on the quality of sucuk (Turkish dry-fermented sausage). J Sci Food Agric. 2006; 86: 849-856.
Radmehr B and Abdolrahimzade M. Antimicrobial effects of Sumac (Rhus coriaria L.) extract in minced meat. Planta Medica. 2009; 75: 1068.
Product brochures, indena. Available at: http://www.indena.com/pages/brochures.php: Accessed on 21st April 2011.
Moscarella S, Giusti A, Marra F, Marena C, Lampertico M, Relli P, Gentilini P, Buzzelli G. Therapeutic and antilipoperoxidant effects of silybin phosphatidylcholine complex in chronic liver disease: preliminary results. Curr Ther Res. 1993; 53:98-102.
Naik RS, Pilgaonkar VW, Panda VS. Evaluation of antioxidant activity of Ginkgo biloba phytosomes in rat brain. Phytother Res. 2006; 20 (11): 1013-1016.
Mukherjee K, Maiti K, Venkatesh M, Mukherjee PK. Phytosome of Hesperetin, a value added formulation with phytomolecules. 60th Indian Pharmaceutical Congress. 2008; New Delhi, India. p. 287.
Pierro FD, Menghi AB, Barreca A, Lucarelli M, Calandrelli A. GreenSelect phytosome as an adjunct to a low calorie diet for treatment of obesity: A Clinical Trial. Altern Med Rev. 2009; 2 (14): 154-160.
Naik SR. Hepatoprotective effect of Ginkgoselect Phytosome in rifampicin induced liver injury in rats: evidence of antioxidant activity. Fitoterapia. 2009; 6: 439-445.
Kuamwat RS, Mruthunjaya K, Gupta MK. Preparation, Characterization and Antioxidant Activities of Gallic Acid-Phospholipids Complex. Int J Res Pharm Sci. 2012; 2 (1): 138-148.
Zhang J, Tang Q, Xu X, Li N. Development and evaluation of a novel phytosome loaded chitosan microsphere system for curcumin delivery. Int J Pharma. 2013; 448: 168-174.
Hüsch J, Bohnet J, Fricker G, Skarke C, Artaria Ch. Enhanced absorption of boswellic acids by a lecithin delivery form (Phytosome) of Boswellia extract. Fitoterapia. 2013; 84: 89-98.
Habbu P, Madagundi S, Kulkarni R, Jadav S, Vanakudri R, Kulkarni V. Preparation and evaluation of Bacopaephospholipid complex for antiamnesic activity in rodents. DIT. 2013; 5: 13-21.
Omar Ali Sh, Abd El-Moeti Darwish H, Abd El-Fattah Ismail N. Modulatory effects of curcumin, silybin-phytosome and alpha-R-lipoic acid against thioacetamide-induced liver cirrhosis in rats. Chem Biol Interact. 2014; 216: 26-33.
Bhattacharyya S, Majhi S, Pada Saha B, Mukherjee PK. Chlorogenic acid-phospholipid complex improve protection against UVA induced oxidative stress. J Photochem Photobiol B: Biol. 2014; 130: 293-298.
Wu H, Long X, Yuan F, Chen L, Pan S, Liu Y, Stowell Y, Li X. Combined use of phospholipid complexes and self-emulsifying microemulsions for improving the oral absorption of a BCS class IV compound, baicalin. Acta Pharmaceutica Sinica B. 2014; 4 (3): 217-226.
Shivanand P, Kinjal P. Phytosome: thechnical revolution in phytomedicine. Int J PharmTech Res. 2010; 2(1): 627-631.
Bombardelli E, Curri SB, Garibldi P. Cosmetic utilization of complexes of Panax ginseng saponins with phospholipids in phytosome form. Fitoterapia. 1989; 60: 55-70.
Mohammadi M, Ghanbarzadeh B, Hamishehkar H, Rezayi mokarram R, Mohammadifar MA. Assesmet of colloidal properties of vitamin D3 producted by thin layer- hydration- sonication method. Iran Nutr Food Sci J. 2013; 4: 175-188. (In Persian).
Bashiri S, Ghanbarzadeh B, Hamishehkar H. Assesmet of colloidal properties of beta carotene producted by thin layer- hydration- sonication method. 2012. 21st National Congress of Food Science and Technology. Iran. Shiraz. 2013. (In Persian).
Tizchang S, Sotikhiyabani M, Rezaii mokarram R, Ghanbarzadeh B, Javadzadeh Y. Assessment of
physical properties of nysine nano liposome. Iran Nutr Food Sci J. 2012; 68-59. (In Persian).
Pezeshki AM, Ghanbarzadeh B, Hamishehkar H. Comparition of colloidal properties of nanoemulsions, nanoliposomes and nano lipid carriers containing vitamin A palmitate. 2014. PhD Thesis. Tabriz University.
Babazadeh A, Ghanbarzadeh B, Hamishehkar H. Comparition of colloidal properties of nanophytosomes and nano lipid carriers containing rutin. 2014. MSc Thesis. Tabriz University.
Cui W. H, Quan X, Tao K, Teng Y, Zhang XG, Liu Y, Shi G, Hou T. Mechanism of action of neomycin on Erwiniacarotovora subsp. carotovora. Pestic Biochem and Physiol. 2009; 95: 85-89.
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