Natural Pigment Production by Monascus purpureus: Bioreactor Yield Improvement through Statistical Analysis
Applied Food Biotechnology,
Vol. 2 No. 2 (2015),
20 March 2015
Among the ways of pigment production, microbial synthesis has gained more interest for high growth rate, easy extraction and high yield. Pigments are used in the food industry as natural colorants and preservatives; they also have pharmaceutical applications. In this study, fungus Monascus purpureus PTCC 5303 was used to produce red, orange and yellow pigments. At first, significant variables were screened based on Plackett-Burman’s design. The optimized value of two effective factors, i.e. concentration of yeast extract and K2HPO4 by three-level, was more studied by the response surface method (RSM). The most suitable level was 2.75 g/L for yeast extract and 1.5 g/L for K2HPO4. Antimicrobial activity of the pigments was shown on Gram-positive food-borne bacteria under optimal conditions. Moreover, pigment production at optimal conditions in a bioreactor was evaluated, and the rate of production of red, orange and yellow pigments was obtained to be 2.05, 1.55 and 0.78 (ODU/ml), respectively.
- Antimicrobial property
- Monascu spurpureus
How to Cite
- Unagul P, Wongsa P, Kittakoop P, Intamas S, Srikiti-Kulchai P, Tanticharoen M. Production of red pigments by the insect pathogenic fungus Cordycepsunilateralis BCC 1869. Ind Microbiol Biotechnol. 2005; 32: 135-140.
- Parekh S, Vinci VA, Strobel RJ. Improvement of microbial strains and fermentation processes. Appl Microbiol Biotechnol. 2000; 54: 287-301.
- Erdogrul O, Azirak S. Review of the studies on the red yeast rice (Monascuspurpureus). Turk Elec J Biotechnol. 2004; 2: 37- 49.
- Cheng MJ, Wu MD, Chen IS, Yuan GF. A new sesquiterpene isolated from the extracts of the fungus MonascusPilosus-fermented rice. Nat Prod Res. 2010; 24: 750-758.
- Hong SH, Lee I, Kim SJ, Imm JY. Improved functionality of soft soybean curd containing Monascus fermented soybean ethanol extract. Food Sci Biotechnol. 2012; 21: 701-707.
- Patakova P. Monascus secondary metabolites: Production and biological activity. Ind Microbiol Biotechnol. 2012; 40: 169-181.
- Feng Y, Shao Y, Chen F. Monascus Pigments. Appl Microbiol Biotechnol. 2012; 96: 1421-1440.
- Lin YL, Wang TH, Lee MH, Su NW. Biologically active components and nutraceuticals in the Monascus-fermented rice: A review. Appl Microbiol Biotechnol. 2008; 77: 965-973.
- Vidyalalakshmi R, Paranthaman R, Murugesh S, Singaravadivel K. Stimulation of Monascus pigments by intervention of different nitrogen sources. Global J Biotechnol Biochem. 2009; 4: 25-28.
- Shi YC, Pan TM. Beneficial effects of Monascuspurpureus NTU 568-fermented products: A review. Appl Microbiol Biotechnol. 2011; 90: 1207-1217.
- Ajdari Z, AbdGhani M, Khan Ayob M, Bayat S, Mokhtar M, Abbasiliasi S, Khoramnia A, Sulaiman Rahman H, Mehrbod P, Ajdari D, Ariff AB. Hypocholesterolemic Activity of Monascus Fermented Product in the Absence of Monacolins with Partial Purification for Functional Food Applications. Sci World J. 2014; 1-12.
- Ajdari Z, Ebrahimpour A, Abdul Manan M, Hamid M, Mohamad R, Ariff AB. Nutritional Requirements for the Improvement of Growth and Sporulation of Several Strains of Monascuspurpureus on Solid State Cultivation. Biomed Biotechnol. 2011; 1-9.
- Yang S, Zhang H, Li Y, Qian J, Wang W. The ultrasonic effect on biological characteristics of Monascus sp. Enzyme Microb Technol. 2005; 37: 139-144.
- Vendruscolo F, Tosin I, Josegiachini A, Schmidell W, Luizninov J. Antimicrobial activity of monascus pigments production in submerged fermentation. Food Process Preserv. 2013; 1-6.
- Elisashvili V. Submerged cultivation of medicinal Mushrooms: Bioprocesses and products (review). Int J MedMushrooms. 2012; 14: 211-239.
- Kennedy M, Krouse D. Strategies for improving fermentation medium performance: A review. Ind Microbiol Biotechnol. 1999; 23: 456-475.
- Yu X, Hallett SG, Sheppard J, Watson AK. Application of the Plackett–Burman experimental design to evaluate nutritional requirements for the production of Colletotrichumcoccodes spores. Appl Microbiol Biotechnol. 1997; 47: 301-305.
- Panda BP, Javad S, Ali M. Fermentation Process optimization. Res JMicrobiol. 2007; 2: 201-208.
- Anita S, Narsi RB. Optimization of ethanol production from microwave alkali pretreated rice straw using statistical experimental designs by Saccharomyces cerevisiae. Ind Crop Prod. 2012; 37, 334-341.
- Anuradha SJ, Valli Nachiyar C. Utilization of pretreated bagasse for the sustainable bioproduction of cellulase by Aspergillusnidulans MTCC 344 using response surface methodology. Ind Crop Prod. 2011; 34: 1564-1571.
- Miyake T, Kono I, Nozaki N, Sammoto H. Analysis of pigment compositions in various Monascus cultures. Food SciTechnol Res. 2008; 14: 194-197.
- Schmitt M, Blanc P. Innovative aspects in biotechnology of eukaryotes: Biotechnology of fungi. BioINEP published series, National Bank of industrial microorganisms and cell culture. 2001; 30-45.
- Mukherjee G, Kumar Singh S. Purification and characterization of a new red pigment from Monascuspurpureus in submerged fermentation. ProcessBiochem. 2011; 46: 188-192.
- Chen MH, Johns M. Effect of pH and nitrogen source on pigment production by Monascuspurpureus. Appl Microbiol Biotechnol. 1993; 40: 132-138.
- Plackett RL, Burman JP. The design of optimum multifactorial experiments, Biometrika. 1946; 33: 305-325.
- Velmurugan P, Kamala-Kannan S, Balachandar V, Lakshmanaperumalsamy P, Chae JC, Oh BT. Natural pigment extraction from five filamentous fungi for industrial applications and dyeing of leather. Carbohydr Polym. 2010; 79: 262-268.
- National Committee for Clinical Laboratory Standards (2006) Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically, Approved Sandard, 7th Ed. 23.
- Sharmila G, Nidhi B, Muthukumaran C. Sequential statistical optimization of red pigment production by Monascuspurpureus (MTCC 369) using potato powder. Ind Crop Prod. 2013; 44: 158-164.
- Chatterjee S, Maity S, Chattopadhyay P, Sarkar A, Laskar S, Kumar Sen S. Characterization of Red Pigment from Monascus in Submerged Culture Red Pigment from Monascus Purpureus. Appl Sci Res. 2009; 5: 2102-2108.
- Kim C, Jung H, Kim JH, Shin CS. Effect of monascuspigment derivatives on the electrophoretic mobility of bacteria, and the cell adsorption and antibacterial activities of pigments. Colloids. Surf. B. Biointerfaces. 2006b; 47: 153-159.
- Kim C, Jung H, Kim YO, Shin CS. Antimicrobial activities of amino acid derivatives of Monascus pigments. FEMS MicrobiolLett.2006a; 264:117-124.
- Lee BK, Park NH, Paio HY, Chung WJ. Production of Red Pigments by Monascuspurpureusin Submerged Culture. Biotechnol Bioprocess Eng.2001;6: 341-346.
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