Vol. 12 No. 1 (2025)

Optimization of Medium Composition and Fermentation Conditions to Maximize Viable Cells and Biomass Production of Lactiplantibacillus plantarum DLBSK207 Using Response Surface Methodology

Applied Food Biotechnology, Vol. 12 No. 1 (2025), 4 January 2025 , Page 1-15 (e3)
https://doi.org/10.22037/afb.v12i1.45961

Abstract

Background and Objective: The aim of this study was to increase the viable cell and biomass production of a potential probiotic strain, Lactiplantibacillus plantarum DLBSK207, by optimizing the ideal concentrations of key nutrients and fermentation conditions parameters using statistical method. such as (RSM) with Box-Behnken Design (BBD).

Material and Methods: The experiments investigated two key variables for medium composition and fermentation conditions. Based on the OFAT result, six factors were selected for the Plackett-Burman Design to evaluate whether the variables had significant effects to the response. The medium contains carbon (glucose) and nitrogen sources (yeast extract and peptone), while the fermentation conditions include initial pH and temperature. The basal medium, consisting of sodium acetate, MgSO4 7H2O, K2HPO4, MnSO4 H2O, and Tween 80, was kept constant. Using RSM, the concentrations of glucose, yeast extract, and peptone, as well as the initial pH and temperature, were optimized to maximize viable cell counts and biomass.

Results and Conclusion: The optimum medium concentrations determined by RSM were 33.76 g l˗1 glucose, 32.59 g l˗1 yeast extract, and 28.38 g l˗1peptone at an initial pH of 6.0 and a temperature of 35 °C. Under these optimized conditions, this study achieved a viable cell counts of 9.30 log CFU.ml˗1 and a dry cell weight of 4.319 g l˗1, representing a 1.82-fold increase compared to standard MRS broth. The experimental results were in closely matched the predicted values of 9.30 log CFU.ml˗1 and 4.280 g l˗1. Scaling up the process in a 10-l bioreactor controlled at pH 6.0 resulted in even higher biomass production, reaching a maximum viable cell counts of 9.88 log CFU.ml˗1 and a dry cell weight of 5.819 g l˗1after 20 h of incubation.  

Conflict of interest: The authors declare no conflict of interest.

Keywords:
  • Box-Behnken Design
  • Controlled pH
  • Plackett-Burman Design
  • Probiotic
  • Scaling up
Optimized medium and conditions of Lactiplantibacillus plantarum

How to Cite

Silaban, B. J. S., Nuraida, L., Sitanggang , A. B., & Tjandrawinata, R. R. (2025). Optimization of Medium Composition and Fermentation Conditions to Maximize Viable Cells and Biomass Production of Lactiplantibacillus plantarum DLBSK207 Using Response Surface Methodology. Applied Food Biotechnology, 12(1), 1–15 (e3). https://doi.org/10.22037/afb.v12i1.45961

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