Optimization of Monascus Pigment Production on Date Waste Substrates Using Solid State Fermentation
Applied Food Biotechnology,
Vol. 8 No. 3 (2021),
15 June 2021
,
Page 247-254
https://doi.org/10.22037/afb.v8i3.34278
Abstract
Background and Objective: Monascus purpureus can produce pigments with light yellow to dark red colors. It includes several health properties. In this study, a low-cost process has been developed for the production of natural pigments from filamentous fungi through solid-state fermentation using cheap substrates.
Material and Methods: In this study, cultivation conditions were optimized for the production of red Monascus pigment by Monascus purpureus ATCC16362 using response surface methodology. Incubation time (7-24 days), date waste syrup concentrations (1-69%) and NaCl contents (7-13.75 g.l-1) were analyzed base on central composite design.
Results and Conclusion: The maximum production of red Monascus pigment (5.10 AU.g-1) by Monascus purpureus was achieved using 55% date waste syrup concentration, 7 g.l-1 NaCl and incubation time of 21 days. At optimum conditions, µmax of 6.2 × 10-3 (mg.g-1.h-1), pigment efficiency of 0.238 (AU.g-1.day-1), conversion factor of biomass in red pigments of 0.25 (AU.mg-1.g-1), glucose utilization of 93% were achieved. Results showed that use of date waste syrup and wheat straw as substrates were successful in solid state cultivation for the production of red pigments by Monascus purpureus.
Conflict of interest: The authors declare no conflict of interest.
- ▪ Date waste syrup ▪ Monascus purpureus ▪ Red pigment ▪ Response surface methodology ▪ Wheat straw
How to Cite
References
Heer K, Sharma S. Microbial pigments as a natural color: A review. Int J Pharm Sci. 2017; 8: 1913-1922.
doi: 10.13040/IJPSR.0975-8232.8(5).1913-22
Makhmur A, Bibhu Prasad P. Optimization of red pigment production by Monascus purpureus MTCC 369 under solid-state fermentation using response surface methodology. Songklanakarin J Sci Technol. 2014; 36: 439-444.
Seyedin A, yazdian F, Hatamian-Zarmi A, Rasekh B, Mirderikvand M. Natural pigment production by Monascus purpureus: Bioreactor yield improvement through statistical analysis. Appl Food Biotechnol. 2017; 2(2): 23-30.
doi: 10.22037/afb. v2i2.7457
Agboyibor C, Kong W, Chen D, Zhang A, Niu, S. Monascus pigments production, composition, bioactivity and its application: A review. Biocatal Agric Biotechnol. 2018; 16: 433-447.
doi: 10.1016/j.bcab.2018.09.012
Wongjewboot I, Kongruang S. pH stability of ultrasonic Thai isolated Monascus Purpureus pigments. Int J Biosci Biochem Bioinforma. 2011; 1: 79-83.
doi: 10.7763/IJBBB.2011.V1.15
Gomah N, Abdel-Raheam H. Stability and components identification of Monascus ruber´s pigments. J Food Dairy Sci. 2017; 8: 31-36.
doi: 10.21608/jfds.2017.37109
Pengnoi P, Mahawan R, Khanongnuch Ch, Lumyong S. Antioxidant properties and production of monacolin K, citrinin, and red pigments during solid state fermentation of purple rice (Oryzae sativa) varieties by Monascus purpureus. Czech J Food Sci. 2017; 35: 32-39.
doi: 10.17221/154/2016-CJFS
Dogra P, Kumar D. Characterization of Monascus purpureus isolated from red yeast rice and its evaluation for the production of cholesterol lowering lovastatin. Biol Forum Int J. 2017; 9: 70-76.
Kim D, Ku S. Beneficial effects of Monascus sp. KCCM 10093 pigments and derivatives: A mini review. Molecules. 2018; 23: 1-15.
doi: 10.3390/molecules23010098.
Lin CP, Lin YL, Huang PH, Tsai HS, Chen YH. Inhibition of endothelial adhesion molecule expression by Monascus purpureus fermented rice metabolites, monacolin K, ankaflavin and monascin. J Sci Food Agric. 2011; 91: 1751-1758.
doi: 10.1002/jsfa.4371.
Nateghi L, Maleki Kahaki A, Zarei F. The effect of replacing sodium nitrite with Monascus purpureus pigment in german sausage. J Nutri Food Secur. 2020; 5 (4):335-344.
doi:10.18502/jnfs. v5i4.4435
Harini S, Kumaresan R. Production of cellulase from corn cobs by Aspergillus niger under submerged fermentation. Int J Chem Tech Res. 2014; 6: 2900-2904.
Shata HMA, Noor El Deen AM, Abdelwahed NAM, Farid M. Statistical optimization of erythromycin production by Saccharopolyspora erythraea under solid state fermentation of agro-industrial materials using response surface methodology. J microbiol biotechnol food sci. 2018; 8: 692-697.
doi:10.15414/jmbfs.2018.8.1.692-697
Khosravi-Darani K, Zoghi A. Comparison of pretreatment strategies of sugarcane baggase: Experimental design for citric acid production. Bioresour Technol. 2008; 99: 6986-6993.
doi: 10.1016/j.biortech.2008.01.024
Subhasree RS, Dinesh Babu P, Vidyalakshmi R, Chandra Mohan V. Effect of carbon and nitrogen sources on stimulation of pigment production by Monascus purpureus on jackfruit seeds. Int J Microbiol Res. 2011; 2: 184-187.
deAlmeida AB, Santos AH, de Lima TM, Santana RV, de Oliveira Filho JG, Peres DS, Egea MB. Pigment bioproduction by Monascus purpureus using corn bran, a byproduct of the corn industry. BiocatalAgric Biotechnol. 2021; 32:101931
doi: 10.1016/j.bcab.2021.101931.
Wen Q,Cao X , Chen Z, Xiong Z, Liu J, Cheng Z, Zheng Z, Long C, Zheng B, Huang Z. An overview of Monascus fermentation processes for monacolin K production. Open Chem. 2020; 18(1): 10-21.
doi: 10.1515/chem-2020-0006
Kantifedaki A, Kachrimanidou V, Mullouchos A, Koutinas AA. Orange processing waste valorization for the production of bio-based pigments using the fungal strains Monascus purpureus and Pencillium purpurogenum. J Clean Prod. 2017; 185: 882-890.
doi: 10.1016/j.jclepro.2018.03.03
Nancib N, Nancib A, Boudrant J. Use of waste date products in the fermentative formation of baker's yeast biomass by Saccharomyces cerevisiae. Bioresour Technol. 1997; 60: 67-71.
doi: 10.1016/S0960-8524(97)00004-7
Mazaheri Assadi M, Nikkhah M. Production of citric acid from date Pulp by solid state fermentation. J Agric Sci Technol. 2002; 4: 119-125.
Asadi SZ, Khosravi-Darani K, Nikoopour H, Bakhoda H. Production of Arachidonic acid and Eicosapentaenoic acid by Mortierella alpina CBS 528.72 on date waste. Food Technol Biotechnol. 2018; 56(2):197-207.
doi:10.17113/ftb.56.02.18.5379
Velmurugan P, Hur H, Vellingiri B, Seralathan K, Kui-Jae L, Sang-Myung L, Jong-Chan CH, Patrick JSH, Byung-Taek O. Monascus pigment production by solid-state fermentation with corn cob substrate. J Biosci Bioeng. 2011; 6: 590-594.
doi: 10.1016/j.jbiosc.2011.08.009
Mumtaz R, Bashir S, Zabta Khan Shinwari MN, Ali M. Pigments from soil bacteria and their therapeutic properties: A mini review. Curr Microbiol. 2019: 76 (6): 783-790
doi: 10.1007/s00284-018-1557-2.
Fales FW. The assimilation and degradation of carbohydrates by yeast cells. J Biol Chem. 1951; 193: 113-24.
doi: 10.1016/S0021-9258(19)52433-4
Bas D, Boyaci IH. Modeling and optimization I: Usability of response surface methodology. J Food Eng.2007; 78: 836-845.
doi:10.1016/j.jfoodeng.2005.11.024
Kumar V, Ahluwalia V, Saran S, Kumar J, Patel AK, Singhania RR. Recent developments on solid-state fermen-tation for production of microbial secondary metabolites: Cha-llenges and solutions. Bioresour Technol. 2021. 323:124566.
doi: 10.1016/j.biortech.2020.124566
Rashmi D, Padmavthi T. Monascus purpureus: A potential source for natural pigment production. J Microbiol Biotech Res. 2011; 1: 164-174.
Babitha S, Soccol CR, Pandey A. Solid-state fermentation for the production of Monascus pigments from jackfruit seed. Bioresour Technol. 2007; 98:1554-1560.
doi: 10.1016/j.biortech.2006.06.005
Keivani H., Jahadi M., Ghasemisepero N. Optimizing submerged cultivation for the production of red pigments by Monascus purpureus on soybean meals using response surface methodology. Appl Food Biotechnol. 2020; 7(3):143-152.
doi: 10.22037/afb.v7i3.28931
Farhan Binti MS. Monascus ruber ICMP 15220 fermentation for the production of pigments. A Thesis Presented in Partial Fulfilment of the Requirements for the Degree of Doctor of Philosophy in Bioprocess Engineering at Massey University, New Zealand; 2010
Al Aamin Razali M, Farhan MS. red pigment production by Monascus purpureus in stirred-drum bioreactor. Sci Herit J. 2017; 1(1): 13-15.
doi: 10.26480/gws.01.2017.13.15
Farhan MS. John B, Chisti Y. Optimal C: N ratio for the production of red pigments by Monascus ruber. World J Microbiol Biotechnol. 2014; 30:2471-2479.
doi:10.1007/s11274-014-1672-6
Hamdi M, Blanc PJ, Loret MO, Goma G. A new process for red pigment production by submerged culture of Monascus purpureus. Bioprocess Eng. 1997; 17: 75-79.
doi:10.1007/pl00008958
Mousa ShA, Abdou DAM, Mohamed GA, Abo-El-Seoud MA, KaramEldin AZA, El-mehalawy AA. Production of red pigment by Monascus purpureus Nrrl 1992 under submerged and solid-state fermentation. Egypt J Microbiol. 2018; 53: 83-94.
doi: 10.21608/ejm.2018.3962.1059
- Abstract Viewed: 1172 times
- pdf Downloaded: 795 times