Chitin Extraction from Lobster Shell Waste using Microbial Culture-based Methods
Applied Food Biotechnology,
Vol. 5 No. 3 (2018),
5 July 2018
,
Page 141-154
https://doi.org/10.22037/afb.v5i3.20787
Abstract
Background and Objective: Lobster shell waste from seafood processing industry was used as the source of raw material to produce the valuable biopolymer chitin. Chemical and biological treatments of lobster shell waste were performed and compared.
Material and Methods: The chemical method required the use of aqueous solutions of HCl and NaOH. Biological treatment included the use of co-cultures with a protease-producing bacterium, either Bacillus megaterium NH21 or Serratia marcescens db11, and an organic acid-producing bacterium Lactobacillus plantarum. The optimal culture conditions, including co-cultivation strategies and glucose concentrations, were identified to improve efficiency of lobster shell deproteinization and demineralization.
Results and Conclusion: Overall, the successive treatment with a combination of Serratia marcescens db11 and Lactobacillus plantarum resulted in the best co-removal of CaCO3 and proteins and chitin yield (82.56%) from lobster shell biomass, with total deproteinization of 87.19% and total demineralization of 89.59%. The results from the proof-of-concept study described here suggest that microbial treatment may be an environmentally friendly alternative to the chemical method of chitin extraction.
- ▪Bacillus megaterium ▪ Chitin extraction ▪ Lactobacillus plantarum ▪ Lobster shell ▪ Serratia marcescens
How to Cite
References
Silva JG. The state of fisheries and aquaculture. FAO. 2014; doi:92-5-105177-1.
Arbia W, Arbia L, Adour L, Amrane A. Chitin extraction from Crustacean shells using Biological methods-review. Food Technol Biotechnol. 2013; 51(1): 12-25.
Xu Y, Gallert C, Winter J. Chitin purification from shrimp wastes by microbial deproteination and decalcification. Appl Microbiol Biotechnol. 2008; 79(4): 687-697. doi: 10.1007/s00253-008-1471-9
Manni L, Ghorbel-Bellaaj O, Jellouli K, Younes I, Nasri M. Extraction and Characterization of Chitin, Chitosan, and Protein Hydrolysates Prepared from Shrimp Waste by Treatment with Crude Protease from Bacillus cereus SV1. Appl Biochem Biotechnol. 2010; 162(2): 345-357. doi: 10.1007/s12010-009-8846-y
Linden JC, Stoner RJ. Pre-harvest application of proprietary elicitor delays fruit senescence. A. Ramina et al. (eds.). Advances in Plant Ethylene Research: Proceedings of the 7th International Symposium on the Plant Hormone Ethylene. 2007; 301-302.
Xuemei Z, Hawkins SJ. Interactions of aquaculture and waste disposal in the coastal zone. Journal of Ocean University of Qingdao. 2002; 1(1), 8-12. doi: 10.1007/s11802-002-0023-7
Wang X, Xing B. Importance of Structural Makeup of Biopolymers for Organic Contaminant Sorption. Environ Sci Technol. 2007; 41(10): 3559-3565. DOI: 10.1021/es062589t
Rinaudo M. Chitin and chitosan: Properties and applications. Prog Polym Sci. 2006; 31(7): 603-632. 10.1016/j.progpolymsci.2006.06.001
Kikkawa Y, Tokuhisa H, Shingai H, Hiraish H, Kanesato M, Imanaka T, Tanaka T. Interaction Force of Chitin-Binding Domains onto Chitin Surface. Biomacromolecules. 2008; 9(8): 2126-2131. DOI: 10.1021/bm800162x
Einbu A, Varum KM. Characterization of Chitin and Its Hydrolysis to GlcNAc and GlcN. Biomacromolecules. 2008; 9(7): 1870-1875. DOI: 10.1021/bm8001123
Ruocco N, Costantini S, Guariniello S, Costantini M. Polysaccharides from the Marine Environment with Pharmacological, Cosmeceutical and Nutraceutical Potential. Molecules. 2016; 21(5). doi: 10.3390/molecules21050551
Khorrami M, Najafpour GD, Younesi H, Hosseinpour MN. Production of chitin and chitosan from shrimp shell in batch culture of Lactobacillus plantarum. Chem Biochem Eng. 2012; 26(3): 217-223.
Dai M, Zheng XL, Xu X, Kong XY, Li XY, Gang G, Luo F, Zhao X, Wei YQ, Qian Z. Chitosan-Alginate Sponge: Preparation and Application in Curcumin Delivery for Dermal Wound Healing in Rat. J Biomed Biotechnol. 2009; 595126: 1-8. Doi: 10.1155/2009/595126
Muzzarelli RAA. Chitins and chitosans for the repair of wounded skin, nerve, cartilage and bone.Carbohydr Polym. 2009; 76(2): 167-182. Doi: 10.1016/j.carbpol.2008.11.002
Nagahama, H., Maeda, H., Kashiki, T., Jayakumar, R., Furuike, T., Tamura, H. Preparation and Characterization of Novel Chitosan/Gelatin Membranes using Chitosan Hydrogel. Carbohydrate Polymers. Carbohydr Polym. 2009; 76(2): 255-260. Doi: 10.1016/j.carbpol.2008.10.015
Ragetly, G. R., Slavik, G. J., Cunningham, B. T., Schaeffer, D. J., Griffon, D. J. Cartilage tissue engineering on fibrous chitosan scaffolds produced by a replica molding technique. J Biomed Mater Res A. 2010; 93(1): 46-55. doi: 10.1002/jbm.a.32514
Foster LJR, Ho S, Hook J, Basuki M, Marçal H. Chitosan as a Biomaterial: Influence of Degree of Deacetylation on Its Physiochemical, Material and Biological Properties. PLoS ONE. 2015; 10(8): 1-22. doi:10.1371/journal.pone.0135153
Anitha A, Sowmya S, Kumar PTS, Deepthi S, Chennazhi KP, Ehrlich H, Tsurkan M, Jayakumar R. Chitin and chitosan in selected biomedical applications. Progress in Polymer Science. 2014; 39: 1644-1667.
Zhou D, Zhang L, Guo S. Mechanisms of lead biosorption on cellulose/chitin beads. Water Res. 2005; 39(16): 3755-3762. DOI: 10.1016/j.watres.2005.06.033
Bhatnagar A, Sillanpaa M. Applications of chitin- and chitosan-derivatives for the detoxification of water and wastewater--a short review. Adv Colloid Interface Sci. 2009; 152(1-2): 26-38. doi: 10.1016/j.cis.2009.09.003
Muxika A, Etxabide A, Uranga J, Guerrero P, Caba K. Chitosan as a bioactive polymer: Processing, properties and applications. Int J Biol Macromol. 2017; 105(Pt2): 1358-1368. doi: 10.1016/j.ijbiomac.2017.07.087.
Kurita K. Chitin and chitosan: functional biopolymers from marine crustaceans. Biotechnol. 2006; 8(3): 203-26. DOI: 10.1007/s10126-005-0097-5
Younes I, Rinaudo M. Chitin and chitosan preparation from marine sources. Structure, properties and applications. Mar Drugs. 2015; 3(3):1133-74. doi: 10.3390/md13031133.
Yan N, Chen X. Sustainability: Don't waste seafood waste. Nature. 2015; 524(7564):155-7. doi: 10.1038/524155a.
Francisco FC, Simora RMC, Nuñal SN. Deproteination and demineralization of shrimp waste using lactic acid bacteria for the production of crude chitin and chitosan. AACL Bioflux. 2015; 8(1): 107-115.
Hammes WP, Vogel RF. The genus Lactobacillus. In: Wood BJB, Holzapfel WH(ed.), The genera of lactic acid bacteria. Glasgow: Blackie Academic & Professional, 1995: 19-54.
Yang JK, Shih I, Tzeng YM, Wang SL. Production and purification of protease from a Bacillus subtilis that can deproteinize crustacean wastes. Enzyme Microb Technol. 2000: 26(5-6): 406-413.
Wang SL, Chio SH. Deproteinization of shrimp and crab shell with the protease of Pseudomonas aeruginosa K-187. Enzyme Microb Technol. 1998; 22 (7): 629-633.
Jo GH, Jung WJ, Kuk JH, Oh KT, Kim YJ, Park RD. Screening of protease-producing Serratia marcescens FS-3 and its application to deproteinization of crab shell waste for chitin extraction. Carbohydr Polym. 2008; 74(3): 504-508.
Zakaria Z, Hall GM, Shama G. Lactic acid fermentation of scampi waste in a rotating horizontal bioreactor for chitin recovery. Process Biochem. 1998; 33(1): 1-6. Doi: 10.1016/S0032-9592(97)00069-1
Bautista J, Jover M, Gutierrez JF, Corpas R, Cremades O, Fontiveros E, Iglesias F, Vega J. Preparation of crayfish chitin by in situ lactic acid production. Process Biochem. 2001; 37(3): 229-234. 10.1016/S0032-9592(01)00202-3
Tilman D, Socolow R, Foley JA, Hill J, Larson E, Lynd L, Pacala S, Reilly J, Searchinger T, Sommerville C, Williams R. Energy Beneficial biofuels--the food, energy, and environment trilemma. Science. 2009; 325(5938): 270-271. doi: 10.1126/science.1177970.
Flyg HC, Kenne H. Insect pathogenic properties of Serratia marcescens: phage-resistant mutants with a decreased resistance to Cecropia immunity and a decreased virulence to Drosophila. J Gen Microbiol. 1980; 120(1): 173-181.
Bringel F, Castioni A, Olukoya DK, Felis GE, Torriani S, Dellaglio F. Lactobacillus plantarum subsp. Argentoratensis subsp. nov., isolated from vegetable matrices. Int. J. Syst. Evol. Microbiol. 2005; 55: 1629-1634.
Aytekin O, Elibol M. Cocultivation of Lactococcus lactis and Teredinobacter turnirae for biological chitin extraction from prawn waste. Bioprocess Biosyst Eng. 2010; 33(3): 393-399. Doi: 10.1007/s00449-009-0337-6
Rao MS, Muñoz J, Stevens WF. Critical factors in chitin production by fermentation of shrimp biowaste. Appl Microbiol Biotechnol. 2000; 54(6): 808-813.
Jung W, Park R. Bioproduction of Chitooligosaccharides: Present and Perspectives. Marine Drugs. 2014; 12(11):5328-5356. doi:10.3390/md12115328
Moon GS, Narbad A. Monitoring of Bioluminescent Lactobacillus plantarum in a Complex Food Matrix. Korean J Food Sci Anim Resour. 2017; 37(1): 147-152. doi: 10.5851/kosfa.2017.37.1.147
Romano P, Fabritius H, Raabe D. The exoskeleton of the lobster Homarus americanus as an example of a smart anisotropic biological material. Acta Biomater. 2007; 3(3): 301-309. DOI: 10.1016/j.actbio.2006.10.003
Khor E, Wu H, Lim LY, Guo CM. Chitin-Methacrylate: Preparation, Characterization and Hydrogel Formation. Materials (Basel). 2011; 4(10): 1728-1746. doi:10.3390/ma4101728
Jung WJ, Jo GH, Kuk JH, Kim KY, Park RD. Extraction of chitin from red crab shell waste by cofermentation with Lactobacillus paracasei subsp. tolerans KCTC-3074 and Serratia marcescens FS-3. Appl Microbiol Biotechnol. 2006; 71(2): 234-237. DOI: 10.1007/s00253-005-0126-3
Fagbenro OA. Preparation, properties and preservation of lactic acid fermented shrimp heads. Food Res Int. 1996; 29(7): 595-599. Doi: 10.1016/S0963-9969(96)00077-4
Kaya M, Baran T, Karaarslan M. A new method for fast chitin extraction from shells of crab, crayfish and shrimp. Nat Prod Res. 2015; 29(15): 1477-1480. Doi: 10.1080/14786419.2015.1026341
Kandaiah R, Ramasamy M. Deproteinization of Distillery Yeast Biomass Waste by Protease-producing Bacillus megaterium PB 4.J Bioremediation Biodegrad. 2015; 6(6): 4-11. Doi: 0.4172/2155-6199.1000319
Falini G, Fermani S. Chitin mineralization. Tissue Eng. 2004; 10(1-2): 1-6. DOI: 10.1089/107632704322791646
Guiraud-Guille MM. Plywood structures in nature. Curr Opin Solid State Mater Sci. 1998; 3(3): 221-227. Doi: 10.1016/S1359-0286(98)80094-6
Beaney P, Lizardi-Mendoza J, Healy M. Comparison of chitins produced by chemical and bioprocessing methods. J Chem Technol Biotechnol. 2004; 80(2): 145-15. DOI: 10.1002/jctb.1164
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