Physicochemical Properties and Sensory Evaluation of Reduced Fat Fermented Functional Beef Sausage
Applied Food Biotechnology,
Vol. 4 No. 2 (2017),
5 April 2017
Page pp. 93-102
Background and Objective: Semi-dry fermented sausages were manufactured from beef meat in three types: without starter culture (control), inoculated with Lactobacillus casei and inoculated with Lactobacillus paracasei. Probiotic fermented sausages are safe and healthy meat products, which receive high commercial interest and growing market shares.
Material and Methods: The physico–chemical characteristics (Protein, Moisture, Fat, Ash, Lactic acid value and pH), microbiological features (total aerobic, total mold and yeast and
lactic acid bacteria count) and sensory evaluation (color, flavor, texture and overall acceptability) were analyzed after 0, 10, 20, 30, 40 and 45 days of refrigerated storage at 4°C.
Results and Conclusion: There was a significant difference (p≤0.05) in moisture content, which decreased in all samples during the period of refrigerated storage. However, all other parameters such as protein, fat and ash increased. The lactic acid produced during the fermentation by lactic acid bacteria resulted in a decrease in the pH value of all samples, and improved sensory evaluation of the fermented sausage inoculated with Lactobacillus casei and Lactobacillus paracasei during the storage period. The best results were obtained in the fermented sausage inoculated with Lactobacillus paracasei in physico-chemical, microbial and sensory characteristics. Also we could preserve the product at 4°C for 45 days.
Conflict of interest: The authors declare that there is no conflict of interest.
- ▪ Lactic acid bacteria ▪Production fermented sausage ▪Quality characteristics
How to Cite
Jalarama RK, Pandey MC, Harilal PT, Radhakrishna K.
Optimization and quality evaluation of freeze dried mutton Manchurian. Int J Food Res. 2013; 20(6): 3101-3106.
Desmond E. Reducing salt: A challenge for the meat industry. Meat Sci. 2006; 74(1):188-196. doi:10.1016/j.meatsci.2006-.04.014.
Ray RR, Roy S. Tradition trend and prospect of fermented food products: A brief overview. World J Pharm Pharmaceut Sci. 2014; 3(10): 272-286.
Zhao L, Jin Y, Ma C, Song H, Li H, Wang Z, Xiao S.
Physico-chemical characteristics and free fatty acid
composition of dry fermented mutton sausages as affected by the use of various combinations of starter cultures and spices. Meat Sci. 2011; 88(4):761-766. doi: 10.1016/j.meatsci.2011-.03.010.
Simion AMC, Vizireanu C, Alexe P, Franco I, Carballo J.
Effect of the use of selected starter cultures on some quality, safety and sensorial properties of Dacia sausage: A
traditional romanian dry-sausage variety. Food Control.
; 35(1):123-131. doi:10.1016/j.foodcont.2013.06.047.
Hempel S, Newberry SJ, Maher AR, Wang Z, Miles JN,
Shanman R, Johnsen B, Shekelle PG. Probiotics for the
prevention and treatment of antibiotic-associated diarrhea: a systematic review and meta-analysis. JAMA. 2012; 307(18):1959-1969. doi: 10.1001/jama.2012.3507
Zhang W, Xiao S, Samaraweera H, Lee EJ, Ahn DU.
Improving functional value of meat products. Meat Sci. 2010: 30; 86(1):15-31. doi:0.1016/j.meatsci.2010.04.018.
Ahmed AMH, Elwy LMI. Effect of lactic acid producing
bacteria on some potential pathogens in sausage. J
AssiutVeterinary Medical, 2015; 61(144): 240-247.
Drosinos EH, Paramithiotis S, Kolovos G, Tsikouras I,
Metaxopoulos I. Phenotypic and technological diversity of
lactic acid bacteria and staphylococci isolated from
traditionally fermented sausages in Southern Greece. Food Microbiol. 2007; 24(3):260-270. doi:10.1016/j.fm.2006-.05.001.
Ammor MS, Mayo B. Selection criteria for lactic acid
bacteria to be used as functional starter cultures in dry
sausage production: An update. Meat Sci. 2007; 76(1):138-146. doi: 10.1016/j.meatsci.2006.10.022.
Ahmad S, Rizawi JA, Khan MS, Srivastava PK. Effect of byproduct incorporation on physicochemical and microbiological quality and shelf life of buffalo meat fermented sausage. J Food Process Technol. 2012; 3(195): 1-6. doi:10.4172/2157-7110.1000195.
Nanasombat S, Wimuttigosol P. Control of Salmonella Rissen and Staphylococcus aureus in fermented beef sausage by a combination of cinnamon and mace oils. Kasetsart J Nat Sci. 2012; 46: 620-628.
Bozkurt H, Bayram M. Colour and textural attributes of
sucuk during ripening. Meat Sci. 2006; 73(2):344-350.
Vanderzalt, C., Splittstoesser, DF. Compendium of methods for microbiological examination of food. 3rd. Washington: Publisher by Amer Public Health Assn,1992.
AOAC (Association of Official Analytical Chemists).
Moisture in meat. In: Horwitz, W.and Latimer, GW.(Ed):
Official Methods of Analysis of AOAC International. 18th.
Edition. Maryland,No 950.46. 2006 39: 1.
AOAC (Association of Official Analytical Chemists). Fat
(crude) or ether extract in meat. In: Horwitz, W. and Latimer, GW. (Ed): Official Methods of Analysis of AOAC
International. 18th. Edition. Maryland, No. 960.39. 2006 39: 2.
AOAC (Association of Official Analytical Chemists).
Nitrogen in meat - Kjeldahl method In: Horwitz, W. and
Latimer, GW. (Ed): Official Methods of Analysis of AOAC
International. 18th. Edition. Maryland, No.928.08. 2006 39: 5.
AOAC (Association of Official Analytical Chemists). Ash of meat In: Horwitz, W. and Latimer, GW. (Ed): Official
Methods of Analysis of AOAC International. 18th. Edition.
Maryland, No. 950.153.2006 39: 4.
Wang, FS. Effects of three preservative agents on the shelf life of vacuum packaged Chinese-style sausage stored at 20 C. Meat Sci. 2000; 56(1):67-71.doi: 10.1016/S0309-1740(00)00-022.
AOAC (Association of Official Analytical Chemists). Official Methods of Analysis of AOAC International.25th de Cunnif P. editor, Washington, DC, USA, No. 937.05. 2000: 1-23.
-Al-ahmad S. The effect of starter cultures on the physicochemical, microbiological and sensory characteristics of semi-dried sausages. Int J Chem Tech Res. 2014-2015; 7(4): 2020-2028.
MorettiVM, Madonia G, Diaferia C, Mentasti T, Paleari MA, Panseri S, Pirone G, Gandini G. Chemical and
microbiological parameters and sensory attributes of a typical Sicilian salami ripened in different conditions. Meat Sci. 2004; 66(4):845-854. doi:10.1016/j.meatsci.2003.08.006.
Asmare H, Admassu S. Development and evaluation of dry fermented sausages processed from blends of chickpea flour and beef. East African J Sci. 2013; 7(1):17-30.
Olivares A, Navarro JL, Salvador A, Flores M. Sensory
acceptability of slow fermented sausages based on fat content and ripening time. Meat Sci. 2010; 86(2):251-257.doi:10.1016/j.meatsci.2010.04.005
Zanardi E, Ghidini S, Conter M, Ianieri A. Mineral
composition of italian salami and effect of NaCl partial
replacement on compositional, physico-chemical and sensory parameters. Meat Sci. 2010; 86(3):742-747. doi: /10.1016-/j.meatsci.2010.06.015.
Hemat E, Sheshetawy EL, Safaa MF. Influence of partial
replacement of beef meat by geminated lentil on quality
characteristics of dry fermented sausage. World J of Dairy
and Food Sci. 2010; 2: 197-206.
Bacus JN. Fermented meat and poultry products. Pearson AM, Dutson TR. (Eds.), Advances in meat research, meat and poultry microbiology, London: Macmillan, 1986; 123-164.
Albano H, Van Reenen CA, Todorov SD, Cruz D, Fraga L,
Hogg T, Dicks LM, Teixeira P. Phenotypic and genetic
heterogeneity of lactic acid bacteria isolated from “Alheira”, a traditional fermented sausage produced in Portugal. Meat Sci. 2009; 82(3):389-398.
Al-ahmad Sh, Azizieh A, Yaziji S. The effect of commercial starter cultures on quality parameters of smoked fermented semi-dry sausage. J Agric Sci. ofUniv. Damascus, 2014; 30(1): 253-268.
Ferreira V, Barbosa J, Silva J, Vendeiro S, Mota A, Silva F, Monteiro MJ, Hogg T, Gibbs P, Teixeira P. Chemical and
microbiological characterisation of “Salpicão de Vinhais” and “Chouriça de Vinhais”: traditional dry sausages produced in the North of Portugal. Food Microbiol. 2007; 24(6):618-623.
Fernández-López J, Sendra E, Sayas-Barberá E, Navarro C, Pérez-Alvarez JA. Physico-chemical and microbiological
profiles of “salchichón” (Spanish dry-fermented sausage)
enriched with orange fiber. Mea Sci. 2008; 80(2):410-417.
Bacha K, Jonsson H, Ashenafi M. Microbial dynamics during the fermentation of wakalim, a traditional Ethiopian fermented sausage. J Food Quality. 2010 Jun 1; 33(3):370-390. doi:10.1111/j.1745-4557.2010.00326.x.
Talon R, Lebert I, Lebert A, Leroy S, Garriga M, Aymerich T, Drosinos EH, Zanardi E, Ianieri A, Fraqueza MJ, Patarata L. Traditional dry fermented sausages produced in smallscale processing units in Mediterranean countries and Slovakia. 1: Microbial ecosystems of processing
environments. Meat Sci. 2007; 77(4):570-579. doi: 10.1016-/j.meatsci.2007.05.006.
Afshin J, Saied S, Babak A. Microbial Properties of hot
smoked sausage during shelf life. Global Veterinaria. 2011;
Cygnarowicz‐Provost M, Whiting RC, Craig JC. Steam
surface pasteurization of beef frankfurters. J Food Sci. 1994; 59(1):1-5. doi: 10.1111/j.1365-2621.1994.tb06883.x
Ahmad S, Amer B. Sensory quality of fermented sausages as influenced by different combined cultures of lactic acid bacteria fermentation during refrigerated storage. J Food Process & Technol. 2013; 202:1-8. doi:10.4172/2157-7110.1000202.
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