Enrichment of Probiotic Yogurt with Broccoli Sprout Extract and its Effect on Helicobacter pylori
Applied Food Biotechnology,
Vol. 4 No. 1 (2017),
4 January 2017
Background and Objective: Antibiotic consumption is the main way to cure infection induced by Helicobacter pylori. On the other hand, antibiotics have side effects on human body. So, finding an efficient way to replace antibiotic consumption seems necessary. The aim of this study was to investigate the effect of broccoli sprout extract on the viability of probiotic bacteria and yogurt’s physicochemical properties, and examine the synergistic effect of this extract with probiotics on Helicobacter pylori growth inhibition.
Material and Methods: Four levels of broccoli sprout extract (22.5, 45, 90 and 180 mg ml-1) were prepared and their effect on probiotic yogurt samples was examined. Moreover, their anti- Helicobacter pylori effect was determined.
Results and Conclusion: The research results revealed that Broccoli sprout extract did not have any inhibitory effect on Bifidobacterium lactis and Lactobacillus acidophilus. The variations in acidity of the samples were not significant during storage. A positive correlation was observed between broccoli sprout extract concentration and syneresis. The findings showed the synergistic effect of broccoli sprout extract and probiotics on Helicobacter pylori growth inhibition. Therefore, using broccoli sprout extract and probiotic bacteria, we can produce a yogurt that is effective on the growth inhibition of Helicobacter pylori.
Conflict of interest: The authors declare that there is no conflict of interest.
- ▪ Bifidobacterium lactis ▪ Broccoli sprout extract ▪ Helicobacter pylori ▪ Lactobacillus acidophilus ▪ Probiotic yogurt
How to Cite
Gotteland M, Brunser O, Cruchet S. Systematic review: are probiotics useful in controlling gastric colonization by
Helicobacter pylori? Aliment Pharmacol Ther. 2006; 23:
-1086. doi: 10.1111/j.1365-2036.2006.02868.
Vitor JM, Vale FF. Alternativetherapies for Helicobacter
pylori: probiotics and phytomedicine. FEMS Immunol Med
Microbiol. 2011; 63: 153-164. doi: 10.1111/j.1574-695X.
Vasiljevic T, Shah NP. Probiotics—from Metchnikoff to
bioactives. Int Dairy J. 2008; 18: 714-728. doi: 10.1016/jidairyj. 2008.03.004.
Jimenez-Colmenero F. Potential applications of multiple
emulsions in the development of healthy and functional
foods. Food Res Int. 2013; 52: 64-74. doi: 10.1016/j.foodres.2013.02.040.
Midolo P, Lambert J, Hull R, Luo F. In vitro inhibition of
Helicobacter pylori NCTC 11637 by organic acids and lactic
acid bacteria. J Appl Microbiol. 1995; 79: 475-479. doi:10.
Wang KY, Li SN, Liu CS, Perng DS. Effects of ingesting
Lactobacillus and Bifidobacterium containing yogurt in
subjects with colonized Helicobacter pylori. Am J Clin Nutr.
; 80: 737-741. doi:10.1073/pnas.1102999108/-/DCSupplemental.
Sheu BS, Cheng HC, Kao AW, Wang ST. Pretreatment with Lactobacillus- and Bifidobacterium-containing yogurt can improve the efficacy of quadruple therapy in eradicating residual Helicobacter pylori infection after failed triple therapy. Am J Clin Nutr. 2006; 83: 864-869.
Safamehr A, Mirahmadi M, Nobakht A. Effect of nettle (Urticadioica) Medicinal plant on growth performance, immune responses, and serum biochemical parameters of broiler chickens. Int Res Appl Basic Sci. 2012; 3:721-728.
Malekzadeh F, Ehsanifar H, Shahamat M, Levin M.
Antibacterial activity of black myrobalan (Terminalia chebula Retz) against Helicobacter pylori. Int J Antimicrob Agents,2011; 18: 85-88. doi: 10.1016/S0924-8579(01)00352-1
Shin IS, Masuda H, Naohide K. Bactericidal activity of
wasabi (Wasabia japonica) against Helicobacter pylori. Int J
Food Microbiol. 2004; 94: 255-261. doi: 10.1016/S0168-
Erdogrul ÖT. Antibacterial activities of some plant extracts used in folk medicine. Pharm Biol. 2002; 40: 269-273.doi/pdf/10.1076/phbi.40.4.269.8474
Moreno‐Caselles J, Prats D, Moral R, Dolores Perez‐Murcia M. Effects of Linear Alkylbenzene Sulfonates (LASs) in Sewage Sludge–Amended Soils on Nutrient Contents of
Broccoli Plants. Commun Soil Sci Plan. 2006; 37: 2605-
Fahey JW, Zhang Y, Talalay P. Broccoli sprouts: an
exceptionally rich source of inducers of enzymes that protect against chemical carcinogens. PNAS USA, 1997; 94: 10367-10372.
Ayala G, Escobedo-Hinojosa WI, De La Cruz-Herrera CF,
Romero I. Exploring alternative treatments for Helicobacter pylori infection. World J Gastroenterol. 2014; 20: 1450-1469.doi: 10.3748/WJG.v20.i6.1450.
Eberhardt MV, Kobira K, Keck AS, Juvik JA. Correlation
analyses of phytochemical composition, chemical, and
cellular measures of antioxidant activity of broccoli (Brassica oleracea L. var. italica). J Agric Food Chem. 2005; 53: 7421-7431. doi: 10.1021/jf051495k.
Momtaheni S, Pourahmad R, Akbarian Mooghari A.
Physicochemical, microbial and sensory characteristics of
low-fat stirred yogurt containing Bifidobacterium lactis and
prebiotic compounds. Int J Biol Biotech. 2015; 12: 361- 367.
Sahan N, Yasar K, Hayaloglu A. Physical, chemical and
flavour quality of non-fat yogurt as affected by a β-glucan
hydrocolloidal composite during storage. Food Hydrocolloid, 2008; 22: 1291-1297. doi: 10.1016/j.foodhyd, 2007.06.010.
Thamaraj N, Shah N. Selective enumeration of Lactobacillus delbrueckii ssp.bulgaricus, Streptococcus thermophilus, Lactobacillus acidophilus, bifidobacteria, Lactobacillus casei, Lactobacillus rhamnosus, and propionibacteria. J Dairy Sci. 2003; 86: 2288-2296. doi: 10.3168/jds.S0022-0302(03)73821-1.
Zaika LL. Spices and herbs: their antimicrobial activity and its determination. J Food Safety, 1998; 9: 97-118.
Marhamatizadeh MH, Ehsandoost E, Gholami P. The
influence of Green Tea (Camellia sinensis L.) Extract on
characteristic of probiotic bacteria in milk and yoghurt during fermentation and refrigerated storage. Int J Farm & Alli Sci. 2013; 22: 599-606. doi:10.1021/jf051495k.
Behrad S, Yusof M, Goh K, Baba A. Manipulation of
probiotics fermentation of yogurt by cinnamon and licorice: effects on yogurt formation and inhibition of Helicobacter pylori growth in vitro. Int J Biolog Biomol Agric Food Biotechnolog. Eng. 2009; 3: 563-567.
Cinbas A, Yazici F. Effect of the addition of blueberries on selected physicochemical and sensory properties of yoghurts. Food Technol Biotechnol. 2008; 46: 434-441.
Milanovic SD, Loncar ES, Đuric MS, Malbaša RV. Low
energy Kombucha fermented milk-based beverages. Acta
Periodica Technologica, 2008; 39: 37-46. doi:
Aghajani A, Pourahmad R, Mahdavi Adeli HR. The effect of oligofructose, lactulose and inulin mixture as prebiotic on physicochemical properties of synbiotic yogurt. J Food Biosci Technol. 2014; 4: 33-40.
Lucey JA. Cultured dairy products: an overview of their
gelation and texture properties. Int J Dairy Technol. 2004; 57: 77-84. doi: 10.1111/j.1471-0307.2004.00142.x.
Moghaddam MN. In vitro inhibition of Helicobacter pylori by some spices and medicinal plants used in Iran. Global J Pharm. 2011; 5: 176-180.
Desmond C, Stanton C, Fitzgerald GF, Collins K, Ross RP.
Environmental adaptation of probiotic lactobacilli towards
improvement of performance during spray drying. Int Dairy J. 2001; 11(10), 801-808.
Joung, J.Y., Lee, J.Y., Ha, Y.S., Shin, Y.K., Kim, S.H. & Oh,
N.S. Enhanced Microbial, Functional and Sensory Properties of Herbal Yogurt Fermented with Korean Traditional Plant Extracts. Korean J Food Sci Anim Resour. 2016; 36: 90-99. doi: 10.5851/kosfa.2016.36.1.90.
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