Antioxidant and Antibacterial Activity of Kombucha Beverages Prepared using Banana Peel, Common Nettles and Black Tea Infusions
Applied Food Biotechnology,
Vol. 3 No. 2 (2016),
Backgrounds and Objective: Kombucha is a several thousand years old traditional fermented beverage originated from East. While black tea infusion is the common substrate for preparing kombucha, other herbal infusions can be applied for this reason too. Common medicinal herbs or even waste herbal materials, like banana peel, could be suitable substrates for preparing kombucha analogues. In this study, kombuchas were fermented using nettles leaf and banana peel infusions.
Materials and Methods: Herbal infusions were fermented by kombucha fungi. Folin-Ciocalteu assay was performed to evaluate total phenolic contents; Free radical scavenging activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl. Disk diffusion method was performed to measure inhibitory activity against testing bacteria. All data were statistically analyzed by ANOVA test at significant level of p≤0.05.
Results and Conclusion: Black tea contained highest amount of phenolics (530.5 ppm gallic acid equivalent) and fermentation decomposed approximately 50% of phenolic contents to 265.5 ppm while phenolic content of nettles infusion and fermented beverage were 173 gAE and 188 gAE respectively and for banana peel, 136.5 gAE and 155 gAE; it indicated increase of phenolic contents due to fermentation that may be cause of protein contents of nettles and banana peel gone under fermentation by lactic acid bacteria. Fermented beverage of three herbs had higher antioxidant potent than infusions. Kombucha from banana peel showed the highest antioxidant activity by inhibiting 94.62% of DPPH. While antioxidant activity of fermented beverages of black tea and nettles leaf were more related to their acetic acid content, it was found that a considerable part of antioxidant activity of banana peel kombucha was due to other acids and phenolics. No antibacterial activity was observed from either of samples. Banana peel, as a waste herbal material, and nettles leaf are good ingredients for being used as substrate to make antioxidant kombucha beverage.
- banana peel
- black tea
- nettles leaf
- phenolic content
How to Cite
. Anken Ralf H, Kappel T. Histochemical and anatomical observations upon the tea fungus. Eur Arch Biol. 1992; 103(4): 219-222.
Battikh H, Bakhrouf A, Ammar E. Antimicrobial effect of Kombucha analogues. Lebensm Wiss Technol. 2012; 47 (1): 71-77.
. Dufresne C, Farnworth E. Tea, kombucha and health: a review. Food Res Int. 2000; 33 (6): 409-421.
. Greenwalt CJ, Steinkraus KH, Ledford RA. Kombucha, the fermented tea: microbiology, composition, and claimed health effects. J Food Prot. 2000; 63 (7): 976-981.
Hartmann AM, Burleson LE, Holmes AK, Geist CR. Effects of chronic kombucha ingestion on open-field behaviors, longevity, appetitive behaviors, and organs in C57-BL/6 mice: a pilot study. Nutrition. 2000; 16 (9): 755-761.
. Greenwalt CJ, Steinkraus KH, Ledford RA. Determination and characterization of the antimicrobial activity of the fermented tea kombucha. Lebensm Wiss Technol. 1998; 13: 291-296.
Sreeramulu G, Zhu Y, Knol W. Characterization of antimicrobial activity in Kombucha fermentation. Acta Biotechnol. 2001; 21: 49-56.
. Graham HN. Green tea composition, consumption, and polyphenol chemistry. Prev Med. 1992; 21 (3): 334-350.
Mukhtar H, Ahmad N. Tea polyphenols: Prevention of cancer and optimizing health. Am J Clin Nutr. 2000; 71 (6 Suppl): 1698-1702.
Gülçin I, Kufrevioglu OI, Oktay M, Buyukokuroglu ME. Antioxidant, antimicrobial, antiulcer and analgesic activities of nettle (Urtica dioica L.). J Ethnopharmacol. 2004; 90 (2-3): 205-215.
Baskar R, Shrisakthi S, Sathyapriya B, Shyampriya R, Nithya R, Poongodi P. Antioxidant Potential of Peel Extracts of Banana Varieties (Musa sapientum). FNS. 2011; 2 (10): 1128-1133.
Kassim Ghaima K, Makie Hashim N, Abdalrasool Ali, S. Antibacterial and antioxidant activities of ethyl acetate extract of nettle (Urtica dioica) and dandelion (Taraxacum officinale). J App Pharm Sci. 2013; 3 (5): 96-99.
Hamilton-Miller JMT. Antimicrobial Properties of Tea (Camellia sinensis L.). Antimicrob Agents Chemother. 1995; 39 (11): 2375–2377.
The European Committee on Antimicrobial Susceptibility Testing. Antimicrobial susceptibility testing-EUCAST disk diffusion method. Version 5.0. 2015.
. Ebrahimi Pure A, Daraei Garmakhany A, Ebrahimi Pure M. The miracle of herbs: Garlic; Biological properties of Allium sativum. Saarbrücken, Germany, 2016; 1st edition: 41-42.
Shahidi F, Naczk M. Phenolics in Food and Nutraceuticals. Taylor & Francis. 2006; 1st edition: 487-488.
. lawrence R, lawrence K. Antioxidant activity of garlic essential oil (Allium Sativum) grown in north Indian plains. Asian Pac J Trop Biomed. 2011; 1 (1): 1-3.
Malbasa RV, Loncar ES, Kolarov LA. TLC analysis of some phenolic compounds in kombucha beverage. APTEFF. 2004; 35: 199-205.
Belgheisi S. Composition change of kombucha during fermentation. 21th national conference on food science and technology. Shiraz, Iran, 2013.
Smith EA, Macfarlane GT. Enumeration of human colonic bacteria producing phenolic and indolic compounds: effects of pH, carbohydrate availability and retention time on dissimilatory aromatic amino acid metabolism. J Appl Bacteriol. 1996; 81 (3): 288-302.
Couto JA, Campos FM, Figueiredo AR, Hogg TA. Ability of Lactic Acid Bacteria to Produce Volatile Phenols. Am J Enol Vitic. 2006; 57 (2): 166-171.
Bossi A, Bonizzato L, Zapparoli G. Acidic extracellular proteases from microrganisms of fairly acidic niche. Protein Pept Lett. 2006; 13 (7): 37-41.
. USDA: National Nutrient Database for Standard Reference Release 28. Basic Report: 35205, Stinging Nettles, blanched (Northern Plains Indians). Available from: https://ndb.nal.usda.gov/ndb/search. [Accessed 5 March 2016].
Velicanski A, Cvetkovic D, Markov S. Characteristics of Kombucha fermentation on medicinal herbs from Lamiaceae family. Rom Biotechnol Lett. 2013; 18 (1): 8034-8042.
Jayabalan R, Marimuthu S, Swaminathan K. Changes in content of organic acids and tea polyphenols during kombucha tea fermentation. Food Chem. 2007; 102: 392-398.
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