In-Vivo Evaluation of Hypoglycemic Activity of Aqueous Extract of Vernonia amygdalina Leaf and its Formulation into Capsule Dosage Form Phytomedicine for hyperglycemic activity
International Pharmacy Acta,
Vol. 9 No. 1 (2026),
11 Mehr 2026
,
Page e3: 1-7
https://doi.org/10.22037/ipa.v9i1.51011
Abstract
Diabetes mellitus is a chronic metabolic disorder characterised by high blood sugar levels due to the body's inability to produce or effectively use insulin. This condition affects millions of people worldwide and can lead to serious health complications if not properly managed. This study evaluates the hypoglycemic activity of aqueous extracts of Vernonia amygdalina leaf and develops a capsule dosage form for its administration. Vernonia amygdalina, commonly known as bitter leaf, is widely used in traditional medicine, particularly for its purported antidiabetic properties. The research involved preparing aqueous extracts from the leaves and administering them to male albino Wistar rats to assess their impact on blood glucose levels. Antidiabetic efficacy was measured by comparing blood glucose levels in extract-treated, glibenclamide-treated, and untreated diabetic rats. Additionally, the study involved formulating the leaf extract into a capsule dosage form to enhance stability and patient compliance. The findings suggest that Vernonia amygdalina has significant hypoglycemic effects, supporting its use as a potential complementary treatment for diabetes mellitus. The encapsulation of the extract also proved to be a viable method for delivering the active components. This study contributes to phytomedicine by exploring alternative treatments for diabetes, highlighting the therapeutic potential of natural products.
- Vernonia amygdalina
- Diabetes Mellitus
- Capsules
- Glibenclamide
- Hypoglycemic
How to Cite
References
1. Syahrian MF, Lister INE, Ginting CN. Evaluating the Therapeutic Potential of Vernonia amygdalina: A Promising Antidiabetic Agent in STZ and Nicotinamide-Induced Phcogj.com Rat Model. Pharmacogn J. 2024;16(1): 94-99.
2. Ajayi, G. O., Edamisan, O. M., Obayemi, P. T., Elegbeleye, E. N., & Obi, E. U. (2021). Phytoconstituents and antidiabetic activity of Vernonia amygdalina (Asteraceae) in streptozotocin-induced diabetic rats. International Journal of Biochemistry, Bioinformatics and Biotechnology Studies, 6, 1-16.
3. Petrie J.R., Chaturvedi N., Ford I., Brouwers M.C.G.J., Greenlaw N., Tillin T., Hramiak I., Hughes A.D., Jenkins A.J., Klein B.E.K., et al. Cardiovascular and metabolic effects of metformin in patients with type 1 diabetes (REMOVAL): A double-blind, randomised, placebo-controlled trial. Lancet Diabetes Endocrinol. 2017;5:597–609.
4. Okafor Ignatius, S. (2023). Formulation and Evaluation of Tablets Containing Extracts of Three Medicinal Plants for Antidiabetic Activity. Int. J. Pharm. Natural Med, 11(1), 23-30.
5. Okokon, J. E., Umoh, F. U., Ekpo, B. A. and Etim, I. E. (2013). Antidiabetic study of combined extracts of Vernonia amygdalina, Ocimum gratissimum, and Gongronema latifolium on alloxan-induced diabetic rats. Journal of Natural Pharmaceuticals, 4(1), 28-31.
6. Ugbogu, E. A., Emmanuel, O., Dike, E. D., Agi, G. O., Ugbogu, O. C., Ibe, C., & Iweala, E. J. (2021). The phytochemistry, ethnobotanical, and pharmacological potentials of the medicinal plant Vernonia amygdalina L.(bitter leaf). Clinical Complementary Medicine and Pharmacology, 1(1), 100006. https://doi.org/10.1016/j.ccmp.2021.100006
7. Chukwuemeka, N. O., Oyebisi, S. O., Adebisi Oluwadare, O. J., Oladapo, R. S., & Berka, N. P. (2018). Antibacterial assay and reversion of carbon tetrachloride induced liver damage on wistar mice by Vernonia amygdalina. Delile. Pakistan journal of pharmaceutical sciences, 31(4).
8. Tunasamy, K., Suryadevara, N., & Athimoolam, T. (2019). Screening of Vernonia amygdalina leaf extracts for antioxidant and antimicrobial activity. Materials Today: Proceedings, 16, 1809-1818. https://doi.org/10.1016/j.matpr.2019.06.055
9. Hasibuan, P. A. Z., Harahap, U., Sitorus, P., & Satria, D. (2020). The anticancer activities of Vernonia amygdalina Delile. Leaves on 4T1 breast cancer cells through phosphoinositide 3-kinase (PI3K) pathway. Heliyon, 6(7). https://doi.org/10.1016/j.heliyon.2020.e04449
10. Alabi, Q. K., & Adeyemi, W. J. (2021). Vernonia amygdalina (Del) as an antioxidant, aspirin toxicity, and oxidative stress. In Toxicology (pp. 491-504). Academic Press.
11. Atangwho, I. J., Edet, E. E., Uti, D. E., Obi, A. U., Asmawi, M. Z., & Ahmad, M. (2012). Biochemical and histological impact of Vernonia amygdalina supplemented diet in obese rats. Saudi Journal of Biological Sciences, 19(3), 385-392.
12. Egedigwe, C. A., Ijeh, I. I., Okafor, P. N., & Ejike, C. E. (2016). Aqueous and methanol extracts of Vernonia amygdalina leaves exert their antiobesity effects through the modulation of appetite-regulatory hormones. Pharmaceutical biology, 54(12), 3232-3236.
13. Tekou, F. A., Kuate, D., Nguekouo, P. T., Woumbo, C. Y., & Oben, J. E. (2018). Effect of cooking treatments on the phytochemical composition and antidiabetic potential of Vernonia amygdalina. Food Science & Nutrition, 6(6), 1684-1691. https://doi.org/10.1002/fsn3.732
14. IfedibaluChukwu, E. I., Aparoop, D., & Kamaruz, Z. (2020). Antidiabetic, anthelmintic and antioxidation properties of novel and new phytocompounds isolated from the methanolic stem-bark of Vernonia amygdalina Delile (Asteraceae). Scientific African, 10, e00578.
15. Erukainure, O. L., Chukwuma, C. I., Sanni, O., Matsabisa, M. G., & Islam, M. S. (2019). Histochemistry, phenolic content, antioxidant, and antidiabetic activities of Vernonia amygdalina leaf extract. Journal of food biochemistry, 43(2), e12737. https://doi.org/10.1111/jfbc.12737
16. Ong KhangWei, O. K., Hsu, A., Song LiXia, S. L., Huang DeJian, H. D., & Tan KwongHuat [Tan, K. (2011). Polyphenols-rich Vernonia amygdalina shows antidiabetic effects in streptozotocin-induced diabetic rats.
17. Aguocha B.U, Ukpabi J.O, Onyeonoro U, Njoku P and Ukegbu, A.U (2013) . Pattern of Diabetes Mortality in a Tertiary Health Facility in South-east Nigeria. African Journal of Diabetes Medicine 21(1): 14-16.
18. Ayodele, A., Adeoye, A. T., Adedapo, A. D., Omobowale, T. O., Adedapo, A. A., & Oyagbemi, A. A. (2017). Antidiabetic and antioxidant activities of the methanol leaf extract of Vernonia amygdalina in alloxan-induced diabetes in Wistar rats. Journal of Medicinal Plants for Economic Development, 1(1), 1-12.
19. Robertson, R.P., 2004. Chronic oxidative stress as a central mechanism for glucose toxicity in pancreatic islet beta cells in diabetes. J. Bol. Chem. 279,42351-42354.
20. Akoko, S., Aleme, B. M., & Uahomo, P. O. (2022). The effect of addition of extracts of Vernonia amygdalina and Moringa oleifera in the nutrition of alloxan-induced diabetic Wistar Rats. International Journal of Pharma Research and Health Sciences, 10(4), 3455-3462.
21. Asante Du-Bois, Emmanuel Effah-Yeboah,1 Precious Barnes, Heckel Amoabeng Abban, Elvis Ofori Ameyaw, Johnson Nyarko Boampong, Eric Gyamerah Ofori and Joseph Budu Dadzie. Antidiabetic Effect of Young and Old Ethanolic Leaf Extracts of Vernonia amygdalina: A Comparative Study. Journal of Diabetes Research 2016; Article ID 8252741.
22. Asante, D. B., Henneh, I. T., Acheampong, D. O., Kyei, F., Adokoh, C. K., Ofori, E. G., ... & Ameyaw, E. O. (2019). Anti-inflammatory, antinociceptive and antipyretic activity of young and old leaves of Vernonia amygdalina. Biomedicine & Pharmacotherapy, 111, 1187-1203.
23. Michael, U. A., David, B. U., Theophine, C. O., Philip, F. U., Ogochukwu, A. M., & Benson, V. A. (2010). Antidiabetic effect of combined aqueous leaf extract of Vernonia amygdalina and metformin in rats. Journal of Basic and Clinical Pharmacy, 1(3), 197.
24. Wu X. M., Ren T., Liu J.F., Liu Y.J., Yang L.C., and Jin X., (2018).Vernonia amygdalina Delle extract inhibits the hepatic gluconeogenesis through the activation of adenosine-5’ monophosph kinase, Biomedicine and Pharmacotherapy. 103, 1384 – 1391.
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