Importance of Cell line Selections from Different Tissues in Cellular Survival Assays
International Pharmacy Acta,
Vol. 1 No. 2 (2018),
17 December 2018
,
Page 184-189
https://doi.org/10.22037/ipa.v1i2.21061
Abstract
Introduction: Cell Culture is a simple and accurate way to evaluate the effect of various factors on different cell lines. One of the most important factors in cell culture is selection of the right cell line for different experiments which might dramatically affect our final conclusions. The purpose of this study was to examine the importance of cell line selection in cellular survival assays.
Methods and Results: To reach this purpose, we used four different cell lines, A2780, A549, HT29 and MIA paca-2, and three widely used pharmaceuticals chemicals. MTT assay was performed in order to evaluate the effect of cisplatin, dexamethasone and progesterone on cell survival after 24 hours exposure. Dexamethasone has proliferation effects on A549, no significant effect on A2780 and MIA paca-2 cell lines, and cytotoxic in high concentrations for HT29 cells. Higher concentrations of progesterone has cytotoxic effect on all four cell lines, furthermore low concentrations of progesterone has proliferative effect on A2780. On the other hand, it did not show any significant effect on A549, HT29 and MIA paca-2 cells in lower concentrations. Cisplatin has cytotoxic effect on all four cell lines with different EC50s.
Conclusions: The results of this study revealed that choosing the proper cell line is important for gaining the reliable results in cell culture experiments.
- Cisplatin
- Dexamethasone
- Progesterone
- A2780
- A549
- HT29
- MIA paca-2
How to Cite
References
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