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  3. Vol. 5 No. 4 (2014): Autumn
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Autumn
Vol. 5 No. 4 (2014)

Comparison of three routine protein precipitation methods: acetone, TCA/acetone wash and TCA/acetone.

  • Naser Nejadi
  • Setareh Mohammadpoor Masti
  • Mostafa Rezaei Tavirani
  • Taghi Golmohammadi

Archives of Advances in Biosciences, Vol. 5 No. 4 (2014),
https://doi.org/10.22037/jps.v5i4.7699 Published 15 December 2014

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Abstract

    The aim of this study was to compare three routine precipitation methods, including: acetone, TCA/acetone wash and TCA/acetone. 30 plasma samples were precipitated using above mentioned three methods. Pellets were dissolved in rehydration solution and protein quantification was done for this solution. According to statistical analysis using SPSS ver 16.0 software and one-way ANOVA test, the protein yield of acetone method was greater than two other methods and was statistically different from TCA/acetone precipitation method (p-value: 0.006). The acetone method is more efficient than two other methods in protein precipitation.

 

Keywords:
  • Protein precipitation
  • Acetone
  • Trichloroacetic acid
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How to Cite

Nejadi, N., Mohammadpoor Masti, S., Rezaei Tavirani, M., & Golmohammadi, T. (2014). Comparison of three routine protein precipitation methods: acetone, TCA/acetone wash and TCA/acetone. Archives of Advances in Biosciences, 5(4). https://doi.org/10.22037/jps.v5i4.7699
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