Tumor Necrosis Factor-Alpha Gene Polymorphism in diabetic patients with Respiratory Failure Undergoing Intensive Care Treatment
Archives of Advances in Biosciences,
Vol. 16 No. 1 (2025),
2 March 2025
,
Page 1-7
https://doi.org/10.22037/aab.v16i1.47424
Abstract
Introduction: Reactive oxygen species (ROS) levels increase in diabetes mellitus due to excessive glucose oxidation. The inflammatory response in cells triggers the activation of proinflammatory cytokines, including tumour necrosis factor (TNF)-α, interleukin (IL)-1, interleukin (IL)-6, and interleukin (IL)-18. Variations in the promoter region of the TNF-α gene can influence both the susceptibility to and severity of the disease. This research examines the correlation between TNF-α gene polymorphisms and the development of respiratory distress in patients with diabetes who are undergoing treatment in an intensive care unit.
Materials and Methods: A total of 100 participants were involved in the study, comprising 50 individuals diagnosed with diabetes and an additional 50 non-diabetic individuals who served as a control group. The nested T-ARMS PCR assay was employed to determine the genotypes associated with the TNF-α T>C single nucleotide polymorphisms (SNPs). Randomly selected samples underwent sequencing to validate the PCR findings, which revealed distinct genotypes for the TNF-α SNPs. Patient data were collected, and laboratory variables were assessed. The information was subsequently entered into SPSS Version 26.
Results: The presence of the heterozygous C/T genotype emerged as a notable risk factor, exhibiting an odds ratio (OR) of 5.87 (65% CI, 1.12 - 27.8) with a p-value of 0.03. In contrast, the homozygous TT genotype did not demonstrate statistical significance, presenting an OR of 1.87 (95% CI, 0.59 - 7.33) and a p-value of 0.25. These findings are derived from the genotyping of the TNF-α (rs1799964) C/T single nucleotide polymorphism (SNP) in a cohort comprising patients experiencing respiratory distress and a control group.
Conclusion: The association between the emergence of respiratory diseases and the TNF-α (rs1799964) T/C polymorphism has been established. This specific genetic variant may represent a potential risk factor for the development of respiratory distress and could also function as a prognostic indicator for patients with diabetes who are undergoing treatment in intensive care units.
- intensive care unit
- Tumor necrosis factor alpha
- respiratory distress
- single nucleotide polymorphism
How to Cite
References
[1] Sandler M, Bunn AE, Stewart RI. Cross-section study of pulmonary function in patients with insulin-dependent diabetes mellitus. Am Rev Respir Dis. 1987;135(1):223-9. [DOI: 10.1164/arrd.1987.135.1.223] [PMID]
[2] Schnapf BM, Banks RA, Silverstein JH, Rosenbloom AL, Chesrown SE, Loughlin GM. Pulmonary function in insulin-dependent diabetes mellitus with limited joint mobility. Am Rev Respir Dis. 1984;130(5):930-2. [DOI: 10.1164/arrd.1984.130.5.930] [PMID]
[3] Lauruschkat AH, Arnrich B, Albert AA, Walter JA, Amann B, Rosendahl UP, et al. Diabetes mellitus as a risk factor for pulmonary complications after coronary bypass surgery. J Thorac Cardiovasc Surg. 2008;135(5):1047-53. [DOI: 10.1016/j.jtcvs.2007.07.066] [PMID]
[4] Keatings VM, Collins PD, Scott DM, Barnes PJ. Differences in interleukin-8 and tumor necrosis factor-alpha in induced sputum from patients with chronic obstructive pulmonary disease or asthma. Am J Respir Crit Care Med. 1996;153(2):530-4. [DOI: 10.1164/ajrccm.153.2.8564092] [PMID]
[5] Takabatake N, Nakamura H, Abe S, Inoue S, Hino T, Saito H, et al. The relationship between chronic hypoxemia and activation of the tumor necrosis factor-alpha system in patients with chronic obstructive pulmonary disease. Am J Respir Crit Care Med. 2000;161(4 Pt 1):1179-84. [DOI: 10.1164/ajrccm.161.4.9903022] [PMID]
[6] Shahsavar F, Varzi AM, Azargoon A. Association between TNF-308G/A polymorphism and susceptibility to pulmonary tuberculosis in the Lur population of Iran. Asian Pac J Trop Biomed. 2016;6(1):80-3. [DOI: 10.1016/j.apjtb.2015.09.017]
[7] Merza M, Farnia P, Anoosheh S, Varahram M, Kazampour M, Pajand O, et al. The NRAMP1, VDR and TNF-alpha gene polymorphisms in Iranian tuberculosis patients: the study on host susceptibility. Braz J Infect Dis. 2009;13(4):252-6. [DOI: 10.1590/S1413-86702009000400002] [PMID]
[8] Shetty BSP, Chaya SK, Kumar VS, Mahendra M, Jayaraj BS, Lokesh KS, et al. Inflammatory biomarkers interleukin 1 beta (IL-1β) and tumour necrosis factor alpha (TNF-α) are differentially elevated in tobacco smoke associated COPD and biomass smoke associated COPD. Toxics. 2021;9(4):72. [DOI: 10.3390/toxics9040072] [PMID]
[9] Yu S, Xue M, Yan Z, Song B, Hong H, Gao X. Correlation between TNF-α -308 and +489 gene polymorphism and acute exacerbation of chronic obstructive pulmonary diseases. Biomed Res Int. 2021;2021:6661281. [DOI: 10.1155/2021/6661281] [PMID]
[10] Ayelign B, Genetu M, Wondmagegn T, Adane G, Negash M, Berhane N. TNF-α (-308) gene polymorphism and type 2 diabetes mellitus in Ethiopian diabetes patients. Diabetes Metab Syndr Obes. 2019;12:2453-9. [DOI: 10.2147/DMSO.S229987] [PMID]
[11] Feng RN, Zhao C, Sun CH, Li Y. Meta-analysis of TNF 308 G/A polymorphism and type 2 diabetes mellitus. PLoS One. 2011;6(4):e18480. [DOI: 10.1371/journal.pone.0018480] [PMID]
[12] Stavros S, Mavrogianni D, Papamentzelopoulou M, Basamakis E, Khudeir H, Psarris A, et al. Association of tumor necrosis factor-α -308G>A, -238G>A and -376G>A polymorphisms with recurrent pregnancy loss risk in the Greek population. Fertil Res Pract. 2021;7(1):9. [DOI: 10.1186/s40738-021-00101-x] [PMID]
[13] Aboutorabi R, Behzadi E, Sadegh MJ, Fatehi SP, Semsarzadeh S, Zarrin Y, et al. The study of association between polymorphism of TNF-α gene's promoter region and recurrent pregnancy loss. J Reprod Infertil. 2018;19(4):211-8. [LINK]
[14] Alkhuriji AF, Alhimaidi AR, Babay ZA, Wary AS. The relationship between cytokine gene polymorphism and unexplained recurrent spontaneous abortion in Saudi females. Saudi Med J. 2013;34(5):484-9. [DOI: 10.15537/1658-3175.5753] [PMID]
[15] Jang HG, Choi Y, Kim JO, Jeon YJ, Rah H, Cho SH, et al. Polymorphisms in tumor necrosis factor-alpha (-863C>A, -857C>T and +488G>A) are associated with idiopathic recurrent pregnancy loss in Korean women. Hum Immunol. 2016;77(6):506-11. [DOI: 10.1016/j.humimm.2016.04.012] [PMID]
[16] El-Tahan RR, Ghoneim AM, El-Mashad N. TNF-α gene polymorphisms and expression. Springerplus. 2016;5(1):1508. [DOI: 10.1186/s40064-016-3197-y] [PMID]
[17] Saxena M, Srivastava N, Banerjee M. Association of IL-6, TNF-α and IL-10 gene polymorphisms with type 2 diabetes mellitus. Mol Biol Rep. 2013;40(11):6271-9. [DOI: 10.1007/s11033-013-2739-4] [PMID]
[18] Guzmán-Flores JM, Muñoz-Valle JF, Sánchez-Corona J, Cobián JG, Medina-Carrillo L, García-Zapién AG, et al. Tumor necrosis factor-alpha gene promoter -308G/A and -238G/A polymorphisms in Mexican patients with type 2 diabetes mellitus. Dis Markers. 2011;30(1):19-24. [DOI: 10.3233/DMA-2011-0759] [PMID]
[19] Andrews C. The Hardy-Weinberg principle. Nat Educ Knowl. 2010;3(10):65. [LINK]
[20] Czarnecka-Chrebelska KH, Mukherjee D, Maryanchik SV, Rudzinska-Radecka M. Biological and genetic mechanisms of COPD, its diagnosis, treatment, and relationship with lung cancer. Biomedicines. 2023;11(2):448. [DOI: 10.3390/biomedicines11020448] [PMID]
- Abstract Viewed: 374 times
- PDF Downloaded: 14 times