The use of bacteriocins, mainly nisin, is one of the most significant preservation technologies in the food industry. Nisin encapsulation can improve stability and homogenous distribution in food matrices. In this study, liposomes of four various lipids (lipoid S 100, lipoid S PC-3, lipoid S PC, and lipoid PC (DPPC)) were prepared by dehydration-rehydration method, and compared for entrapment efficiency, and lipid with the highest entrapment efficiency (DPPC) was characterized. The inhibitory effects of encapsulated (DPPC nanoliposomes) and free nisin on the spoilage of pasteurized milk were also studied. All experiments were performed in triplicate. Entrapment efficiency ranged from 14% (lipoid S 100) to 49% (DPPC). DPPC nanoliposomes were large unilamellar vesicles (LUV), and had an asymmetric oval shape (elliptical) with a mean diameter of 136 nm. It was revealed that pasteurized milk spoilage was delayed by both free and encapsulated nisin, but free nisin (with 38 days) was significantly more efficient in comparison with encapsulated nisin (14 days).
Liu X, Chung Y, Yang S, Yousef AE. Continuous nisin production in laboratory media and whey permeate by immobilized Lactococcus lactis. Process Biochem. 2005; 40: 13-24. DOI: 10.1016/j.procbio.2003.11.032.
Deegan LH, Cotter PD, Hill C, Ross P. Bacteriocins: Biological tools for biopreservation and shelf-life extension. Int Dairy J. 2006; 16: 1058-1071. DOI: 10.1016/j.idairyj.2005.10.026.
Souza EL, Silva CA, Souza CP. Bacteriocins: Molecules of fundamental impact on the microbial ecology and potential food biopreservatives. Braz Arch Biol Technol. 2005; 48: 559-566. DOI: 10.1590/S1516-89132005000500008.
Anton R, Barlow S, Boskou D, Castle L, Crebelli R, Dekant W, Engel K, Forsythe S, Grunow W, Heinonen M, Chr. Larsen J, Leclercq C, Mennes W, Rosaria Milana M, Pratt I, Rietjens I, Svensson K, Tobback P, Toldrá F. Opinion of the scientific panel on food Additives, flavourings, processing aids and materials in contact with food on a request from the commission related to the use of nisin (E234) as a food additive. Eur Food Safety Authority J. 2006; 314, 1-16.
Benech RO, Kheadr EE, Lacroix C, Fliss I. Impact of nisin producing culture and liposome-encapsulated nisin on ripening of Lactobacillus added-Cheddar cheese. J Dairy Sci. 2003; 86: 1895-1909. DOI: 10.3168/jds.S0022-0302(03)73776-X.
Laridi R, Kheadr EE, Benech RO, Vuillemard JC, Lacroix C, Fliss I. Liposome encapsulated nisin Z: Optimization, stability and release during milk fermentation. Int Dairy J. 2003; 13: 325-336. DOI: 10.1016/S0958-6946(02)00194-2.
Sobrino-López A and Martín-Belloso O. Use of nisin and other bacteriocins for preservation of dairy products. Int Dairy J. 2008; 18: 329-343. DOI: 10.1016/j.idairyj.2007.11.009.
Were LM, Bruce B, Davidson PM, Weiss J. Size, stability and entrapment efficiency of phospholipid nanocapsules containing polypeptide antimicrobials. J Agric Food Chem. 2003; 51: 8073-8079. DOI: 10.1021/jf0348368.
Kopermsub P, Mayen V, Warin C. Nanoencapsulation of nisin and ethylenediaminetetraacetic acid in niosomes and their antibacterial activity. J Sci Res. 2012; 4: 457-465. DOI: http://dx.doi.org/10.3329/jsr.v4i2.9407.s.
Benech RO, Kheadr EE, Laridi R, Lacroix C, Fliss I. Inhibition of Listeria innocua in cheddar cheese by addition of nisin Z in liposomes or by in situ production in mixed culture. Appl Environ Microbiol. 2002; 68: 3683-3690. DOI: 10.1128/AEM.68.8.3683-3690.2002.
Ingebrigtsen L. and Brandl M. Determination of the size distribution of liposomes by SEC fractionation, and PCS analysis and enzymatic assay of lipid content. AAPS Pharm Sci Tech. 2002; 3: 1-7 (article 7). DOI: 10.1208/pt030207.
Colas JC, Shi W, Rao VSNM, Omri A, Mozafari MR, Singh H. Microscopical investigations of nisin-loaded nanoliposomes prepared by Mozafari method and their bacterial targeting. Micron 2007; 38: 841-847. DOI: 10.1016/j.micron.2007.06.013.
Taylor TM, Bruce BD, Weiss J, Davidson PM. Listeria monocytogenes and Escherichia coli O157:H7 inhibition in vitro by liposome-encapsulated nisin and ethylenediaminetetraacetic acid. J Food Safety. 2008; 28: 183-197.
Winkowski K, Ludescher RD, Montville TJ. Physicochemical characterization of the nisin-membrane interaction with liposomes derived from Listeria monocytogenes. Appl Environ Microbiol. 1996; 62: 323-327.
Bonev BB, Chan WC, Bycroft BW, Roberts GCK, Watts A. Interaction of the lantibioticnisin with mixed lipid bilayers: A 31P and 2H NMR Study. Biochem. 2000; 39, 11425-11433. DOI: 10.1021/bi0001170.
Wiedemann I, Breukink E, Van Kraaij C, Kuipers OP, Bierbaum G, De Kruijff B, Sahl HG. Specific binding of nisin to the peptidoglycan precursor lipid II combines pore formation and inhibition of cell wall biosynthesis for potent antibiotic activity. J Biol Chem. 2001; 276: 1772-1779. DOI: 10.1074/jbc.M006770200.
Benech RO, Kheadr EE, Lacroix C, Fliss I. Antibacterial activities of nisin Z encapsulated in liposomes or produced in situ by mixed culture during Cheddar cheese ripening. Appl Environ Microbiol. 2002; 68: 5607-5619. DOI: 10.1128/AEM.68.11.5607-5619.2002.
Gao FH, Abee T, Konings WN. Mechanism of action of the peptide antibiotic nisin in liposomes and cytochrome c oxidase-containing proteoliposomes. Appl Environ Microbiol. 1991; 57: 2164-2170. DOI: 0099-2240/91/082164-07.
Pongtharangkul T. and Demirci A. Evaluation of agar diffusion bioassay for nisin quantification. ApplMicrobiolBiotechnol. 2004; 65: 268-272. DOI: 10.1007/s00253-004-1579-5.
Vojta A, Scheuring J, Neumaier N, Mirus O, Weinkauf S, Schleiff E. Determination of liposome size: A tool for protein reconstitution. Anal Biochem. 2005; 347: 24-33. DOI: 10.1016/j.ab.2005.09.003.
Wasiksiri S, Chethanond U, Pongprayoon S, Srimai S, Nasae B. Quality aspects of raw goat milk in lower southern Thailand. Songklanakarin J Sci Technol. 2010; 32: 109-113.
Hassan A, Amjad I, Mahmood S. Microbiological and physicochemical analysis of different UHT milks available in market. Afr J Food Sci. 2009; 3: 100-106.
Milk and milk products Enumeration of colony forming units of microorganisms colony count technique at 30°C. 2007. Institute of Standards and Industrial Research of Iran, ISIRI No. 5484, Tehran, Iran. (http://www.isiri.org, Date of Access: February 23, 2012).
Milk and milk products-General guidance for the preparation of test samples, initial suspensions and decimal dilutions for microbiolgical examination. 2007. Institute of Standards and Industrial Research of Iran, ISIRI No. 2406, Tehran, Iran. (http://www.isiri.org, Date of Access: February 23, 2012).
Schmidt SE, Holub G, Sturino JM, Taylor TM. Suppression of Listeria monocytogenes Scott A in fluid milk by free and liposome-entrapped nisin. Probiotics Antimicro Prot. 2009; 1: 152-158. DOI: 10.1007/s12602-009-9022-y.
Suganthi V, Selvarajan E, Subathradevi C, Mohanasrinivasan V. Lantibioticnisin: Natural preservative from Lactococcuslactis. Int Res J Pharm. 2012; 3: 13-19.
Nayar R, Hope MJ, Cullis PR. Generation of large unilamellar vesicles from long-chain saturated phosphatidylcholines by extrusion technique. BiochimBiophysActa. 1989; 986: 200-206. DOI: 10.1016/0005-2736(89)90468-9.
Mac Donald RC, Mac Donald RI, Menco BPM, Takeshita K, Subbarao NK, Hu LR. Small-volume extrusion apparatus for preparation of large unilamellar vesicles. BiochimBiophysActa. 1991; 1061: 297-303. DOI: 10.1016/0005-2736(91)90295-J.
Mozafari MR, Reed CJ, Rostron C. Cytotoxicity evaluation of anionic nanoliposomes and nanolipoplexes prepared by the heating method without employing volatile solvents and detergents. Pharmazie 2007; 62: 205-209. DOI: 10.1691/ph.2007.3.6045.
Da Silva Malheiros P, Daroit DJ, Da Silveira NP, Brandelli A. Effect of nanovesicle-encapsulated nisin on growth of Listeria monocytogenes in milk. Food Microbiol. 2010; 27: 175-178. DOI: 10.1016/j.fm.2009.09.013.
Zou Y, Lee HY, Seo YC, Ahn J. Enhanced antimicrobial activity of nisin-loaded liposomal nanoparticles against foodborne pathogens. J Food Sci. 2012; 77: M165-M170. DOI: 10.1111/j.1750-3841.2011.02580.x.
Tassew A and Seifu E. Microbial quality of raw cow’s milk collected from farmers and dairy cooperatives in Bahir Dar Zuria and Mecha district, Ethiopia. AgricBiol J N Am. 2011; 2: 29-33. DOI: 10.5251/abjna.2011.2.1.29.33.
Delves-Broughton J. Nisin as a food preservative. Food Aust. 2005; 57:525-527.
Kirby CJ, Brooker BE, Law BA. Accelerated ripening of cheese using liposome-encapsulated enzymes. Int J Food Sci Technol. 1987; 22: 355-375. DOI: 10.1111/j.1365-2621.1987.tb00499.x.
Laloy E, Vuillemard JC, Simard RE. Characterization of liposomes and their effect on the properties of Cheddar cheese during ripening. Le lait. 1998; 78: 401-412. DOI: 10.1051/lait:1998439.
Kheadr EE, Vuillemard JC, El Deeb SA. Accelerated Cheddar cheese ripening with encapsulated proteinases. Int J Food Sci Technol. 2000; 35: 483-495. DOI: 10.1046/j.1365-2621.2000.00398.x.