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Development of Antioxidant Activity during Milk Fermentation by Wild Isolates of Lactobacillus helveticus

Aazam Namdari, Fatemeh Nejati
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Abstract

Background and Objective: Oxidative stress, due to free radicals, brings injury to the body by attacking large molecules and cell organs, and is the main reason of many diseases. Fermentation of foods containing large amount of proteins such as milk by special species of lactic acid bacteria is a potential way in enhancement of the antioxidative activity of foods. This study aimed at evaluating non-common starter species isolates of Lactobacillus helveticus for their capability to produce fermented milk enriched in antioxidant peptides.
Materials and Methods: Reconstituted skim milk (11%) was inoculated with 7 wild isolates of Lactobacillus helveticus, and after 24 h fermentation at 37ºC, the samples were kept 4ºC and for 14 days. Viable cell number, acidification and proteolysis degree in the milk fermented by each isolate were assessed in 1, 7 and 14 days. Development of antioxidant activity was measured using DPPH and ABTS●+ radial scavenging activities during the storage period.
Results and Conclusion: Though some slight strain-dependent differences were observed in growth, acidification and proteolysis, all the samples showed considerably strong antioxidant activity (at least 62.32±3.66% and 57.64±1.42% measured using DPPH and ABTS●+ radicals, respectively) through the whole storage period. In vitro simulated gastrointestinal digestion indicated that DPPH radical-scavenging activity of the antioxidative peptidic supernatants was not affected significantly by consecutive pepsin-pancreatin hydrolysis in most of the samples. These evidences support Lactobacillus helveticus as a promising functional culture able to promote health benefits in dairy-based functional foods.
Conflict of interest: The authors declare that there is no conflict of interest.


Keywords

Antioxidant peptides, Fermentation, Functional, Lactobacillus helveticus, Milk

References

Riley P. Free radicals in biology: oxidative stress and the effects of ionizing radiation. Int J Radiat Biol. 1994; 65(1): 27-33.

Waris G, Ahsan H. Reactive oxygen species: role in the development of cancer and various chronic conditions. J Carcinog. 2006; 5(1): 14-21.

Cooke MS, Evans MD, Dizdaroglu M, Lunec J. Oxidative DNA damage: mechanisms, mutation, and disease. FASEB J. 2003; 17(10): 1195-214.

Pihlanto A. Antioxidative peptides derived from milk proteins. Int Dairy Tech. 2006; 16(11): 1306-14.

Elfahri K, Vasiljevic T, Yeager T, Donkor O. Anti-colon cancer and antioxidant activities of bovine skim milk fermented by selected Lactobacillus helveticus strains. J Dairy Sci. 2015.

Farvin KS, Baron CP, Nielsen NS, Otte J, Jacobsen C. Antioxidant activity of yoghurt peptides: Part 2–Characterisation of peptide fractions. Food Chem. 2010; 123(4): 1090-7.

Chang O, Seol K-H, Jeong S-G, Oh M-H, Park B-Y, Perrin C, Ham J-S. Casein hydrolysis by Bifidobacterium longum KACC91563 and antioxidant activities of peptides derived therefrom. J Dairy Sci. 2013; 96(9): 5544-55.

Kudoh Y, Matsuda S, Igoshi K, Oki T. Antioxidative peptide from milk fermented with Lactobacillus delbrueckii subsp. bulgaricus IFO13953. Jpn Soc Food Sci Tech. 2001; 48(1): 44-50.

Virtanen T, Pihlanto A, Akkanen S, Korhonen H. Development of antioxidant activity in milk whey during fermentation with lactic acid bacteria. J App Microbiol. 2007; 102(1): 106-15.

Solieri L, Rutella GS, Tagliazucchi D. Impact of non-starter lactobacilli on release of peptides with angiotensin-converting enzyme inhibitory and antioxidant activities during bovine milk fermentation. Food Microbiol. 2015; 51: 108-16.

Griffiths MW, Tellez AM. Lactobacillus helveticus: the proteolytic system. Front Microbiol. 2013; 4: 1-9.

Nakamura Y, Yamamoto N, Sakai K, Okubo A, Yamazaki S, Takano T. Purification and characterization of angiotensin I-converting enzyme inhibitors from sour milk. J Dairy Sci. 1995; 78(4): 777-83.

Nejati F, Babaei M, Taghi-Zadeh A. Characterisation of Lactobacillus helveticus strains isolated from home-made dairy products in Iran. Int J Dairy Tech. 2015.

Chen Y, Li C, Xue J, Kwok L-y, Yang J, Zhang H, Menghe B. Characterization of angiotensin-converting enzyme inhibitory activity of fermented milk produced by Lactobacillus helveticus. J Dairy Sci. 2015; 98(8): 5113-24.

Church FC, Swaisgood HE, Porter DH, Catignani GL. Spectrophotometric assay using o-phthaldialdehyde for determination of proteolysis in milk and isolated milk proteins. J Dairy Sci. 1983; 66(6): 1219-27.

Wu H-C, Chen H-M, Shiau C-Y. Free amino acids and peptides as related to antioxidant properties in protein hydrolysates of mackerel (Scomber austriasicus). Food Res Int. 2003; 36(9): 949-57.

Ao J, Li B. Stability and antioxidative activities of casein peptide fractions during simulated gastrointestinal digestion in vitro: Charge properties of peptides affect digestive stability. Food Res Int. 2013; 52(1): 334-41.

Nalinanon S, Benjakul S, Kishimura H, Shahidi F. Functionalities and antioxidant properties of protein hydrolysates from the muscle of ornate threadfin bream treated with pepsin from skipjack tuna. Food Chem. 2011; 124(4): 1354-62.

Kunji ER, Mierau I, Hagting A, Poolman B, Konings WN. The proteotytic systems of lactic acid bacteria. Anton Leeuw Int J G. 1996; 70(2-4): 187-221.

Leclerc P-L, Gauthier SF, Bachelard H, Santure M, Roy D. Antihypertensive activity of casein-enriched milk fermented by Lactobacillus helveticus. Int Dairy J. 2002; 12(12): 995-1004.

Li Y, Liu T, He G. Antioxidant activity of peptides from fermented milk with mix culture of lactic acid bacteria and yeast. Adv J Food Sci Tech. 2015; 7(6): 422-7.

Zevaco C, Gripon J-C. Properties and specificity of a cell-wall proteinase from Lactobacillus helveticus. Le Lait. 1988; 68(4): 393-407.

Lü J-M, Lin PH, Yao Q, Chen C. Chemical and molecular mechanisms of antioxidants: experimental approaches and model systems. J Cell Mol Med. 2010; 14(4): 840-60.

Amarowicz R, Pegg R, Rahimi-Moghaddam P, Barl B, Weil J. Free-radical scavenging capacity and antioxidant activity of selected plant species from the Canadian prairies. Food Chem. 2004; 84(4): 551-62.

Coda R, Rizzello CG, Pinto D, Gobbetti M. Selected lactic acid bacteria synthesize antioxidant peptides during sourdough fermentation of cereal flours. Appl Environ Microbiol. 2012; 78(4): 1087-96.

Sarmadi BH, Ismail A. Antioxidative peptides from food proteins: a review. Peptides. 2010; 31(10): 1949-56.

You L, Zhao M, Regenstein JM, Ren J. Changes in the antioxidant activity of loach (Misgurnus anguillicaudatus) protein hydrolysates during a simulated gastrointestinal digestion. Food Chem. 2010; 120(3): 810-6.




DOI: https://doi.org/10.22037/afb.v3i3.11422

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