Evaluation of the Coagulant Effect of Zanjani and Latifi Viper Snake Venom Endemic in Iran
Trends in Peptide and Protein Sciences,
Vol. 1 No. 1 (2016),
4 October 2016
,
Page 27-30
https://doi.org/10.22037/tpps.v1i1.13130
Abstract
The venom of the viper is very important in pharmaceutical usage, such as in the process of coagulation during medical care. This study aims to evaluate the coagulant factor of Latifi and Zanjani viper venom. In the current study, after electrophoresis of proteins found in viper venom, all of the thick and strong bands of proteins were isolated and prepared for examination of coagulation characteristics, like pro-thrombin time (PTT) and active partial thromboplastic time (APTT), followed by further study by mass spectroscopy. In this way, 11 bands of proteins were recognized in sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). After PT and APTT tests, one common band in 26 KDa could lead to coagulation of blood plasma in less than one second. Mass spectroscopy identified this band as serine protease isoform4. The results confirmed the coagulation effect of a 26 KDa protein fraction of venom from Latifi and Zanjani vipers.
Highlights
- The viper snake venom is mostly blood-oriented and leads to blood coagulation.
- The snakes venom, including vipers venom endemic of Iran could be applied for pharmaceutical purposes.
- A 26KD protein was the most effective component of viper venom showed plasma coagulation.
- The results showed this protein was related to serine protease enzyme of snake venom.
- Latifi viper
- Zanjani viper
- SDS PAGE
- PTT
- APTT
How to Cite
References
Behrooz, R., Kaboli, M., Nourani, E., Ahmadi, M., Shabani, A. A., Yousefi, M., Asadi, A. and M. Rajabizadeh, (2015). "Habitat modeling and conservation of the endemic Latifi’s Viper (Montivipera latifii) in Lar National Park, Northern Iran." Herpetological Conservation and Biology, 10(2): 572-582.
Boyot, P., Pillet, L., Ducancel, F., Boulain, J. C., Tremeau, O. and A. Menez, (1990). "A recombinant snake neurotoxin generated by chemical cleavage of a hybrid protein recovers full biological properties." FEBS Letter, 266(1-2): 87-90.
Clement, H., de Oliveira, V. C., Zamudio, F. Z., Lago, N. R., Valdez- Cruz, N. A., Valle, M. B., Hajos, S. E., Alagón, A., Possani, L. D. and A. R. de Roodt, (2012). "Isolation, amino acid sequence and biological characterization of an “aspartic-49" phospholipase A(2) from Bothrops (Rhinocerophis) ammodytoides venom.” Toxicon, 60(7): 1314-1323. doi: 10.1016/j.toxicon.2012.08.019
Kool, J. (2016). "Pharmaceutical properties of venom toxins and their potential in drug discovery." Indonesian Journal of Pharmacy, 27(1): 1.
Kornalík, F. (1985). "The influence of snake venom enzymes on blood coagulation." Pharmacology & Therapeutics, 29(3): 353-405. doi: http://dx.doi.org/10.1016/0163-7258(85)90008-7
Matsui, T., Fujimura, Y. and K. Titani, (2000). "Snake venom proteases affecting hemostasis and thrombosis." Biochimica et Biophysica Acta (BBA)-Protein Structure and Molecular Enzymology, 1477(1): 146-156.
Pourazin, A., Karami, M. and M. Bandehpour, (2011). "The evaluation the quality of the Endemic Latifi viper snake venom (Montivipera latifi) in order to provide the solution for maintenance in captivity to reduce the fishing and protection of it" (Master Dissertation), Azad University Science and Research Branch, Tehran, Iran.
Rajabizadeh, M., Yazdanpanah, A. and S. Ursenbacher, (2012). "Preliminary analysis of dorsal pattern variation and sexual dimorphism in Montivipera latifii (Mertens, Darevsky and Klemmer, 1967)(Ophidia: Viperidae)." Acta Herpetologica, 7(1): 13-21.
Samy, R. P., Sethi, G. and L. H. K. Lim, (2016). "A brief update on potential molecular mechanisms underlying antimicrobial and wound-healing potency of snake venom molecules." Biochemical Pharmacologyˏ115:1-9. doi:http://dx.doi.org/10.1016/j.bcp.2016.03.006
Wahby, A., Mahdy, E.-S. M., EL-mezayen, H. A., Salama, W. H., Abdel- Aty, A. M. and A. S. Fahmy, (2012). "Egyptian horned viper Cerastes cerastes venom hyaluronidase: Purification, partial characterization and evidence for its action as a spreading factor." Toxicon, 60(8), 1380-1389.
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