Microencapsulation and Fermentation of Lactobacillus acidophilus LA-5 and Bifidobacterium BB-12
食品生物技术的应用,
卷 2 编号 4 (2015),
7 十月 2015
,
第 27-32 页
https://doi.org/10.22037/afb.v2i4.7711
摘要
Because of poor survival of probiotic bacteria, microencapsulation evolved from the immobilized cell culture technology used in the biotechnological industry. Two probiotic strains, Bifidobacterium (BB-12) and Lactobacillus acidophilus (LA-5) were immobilized in calcium alginate by extrusion method. Encapsulation parameters and efficacy of this method were evaluated. Growth factors of these two bacteria were also measured by culturing in 10-L fermenter. Growth curves were obtained with respect to optical density and dry biomass weight. Encapsulation yield was over than 60% in each experiment. Scanning electron microscopy (SEM) of Entrapment of cells in alginate matrix and cross-sections of dried bead were obtained and illustrated. Bifidobacterium have been shown better biotechnological properties.
- Alginate
- Fermenter
- Microencapsulation
- Probiotic
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参考
Vasiljevic T, Shah NP. Probiotics-from Metchnikoff to bioactives. Int Dairy J. 2008; 18: 714-728.
Kaur IP, Chopra K, Saini A. Probiotics: Potential pharmaceutical applications. Eur J Pharm Sci. 2002; 15: 1-9.
Reid G, Burton J. Use of Lactobacillus to prevent infection by pathogenic bacteria. Microbes Infect. 2002; 4: 319-324.
Cremonini F, Di Caro S, Nista EC, Bartolozzi F, Capelli G, Gasbarrini G, Gasbarrini A. Meta-analysis: The effect of probiotic administration on antibiotic associated diarrhoea. Aliment Pharmacol Ther. 2002; 16: 1461-1467.
Gorbach SL. Probiotics and gastrointestinal health. Am J Gastroenterol. 2000; 95(1): 2-4.
Kailasapathy K, Chin J. Survival and therapeutic potential of probiotics organisms with reference to Lactobacillus acidophilus and Bifidobacterium spp. Immunol Cell Biol. 2000; 78(1): 80-88.
Ouwehand AC, Salminen SJ. The health effects of cultured milk products with viable and non-viable bacteria. Int Dairy J. 1998; 8(9): 749-758.
Femia AP, Luceri C, Dolara P, Giannini A, Biggeri A, Salvadori M, Clune Y, Collins KJ, Paglierani M, Caderni G. Antitumorigenic activity of the prebiotic inulin enriched with oligofructose in combination with the probiotics Lactobacillus rhamnosus and Bifidobact-erium lactis on azoxymethane-induced colon carcino-genesis in rats. Carcinog. 2002; 23 (11): 1953-1960.
Rafter J. Lactic acid bacteria and cancer: Mechanistic perspective. Brit J Nutr. 2002; 88: 89-94.
Anal AK, Singh H. Recent advances in microen-capsulation of probiotics for industrial applications and targeted delivery. Trends Food Sci Tech. 2007; 18: 240-251.
Kailasapathy K. Microencapsulation of probiotic bacteria: Technology and potential applications. Curr Issues Intest Microbiol. 2003; 3 (2): 39-48.
De Vos P, Bucko M, Gemeiner P, Navratil M, Svitel J, Faas M, Løkensgard Strand B, Skjak-Braek G, Morch YA, Vikartovska A, Lacık I, Kollarikova G, Orive G, Poncelet D, Pedraz JL, Ansorge-Schumacher MB. Multi-scale requirements for bioencapsulation in medicine and biotechnology. J Biomaterials. 2009; 30: 2559-2570.
Lee JS, Cha DS, Park HJ. Survival of freeze-dried Lactobacillus bulgaricus KFRI 673 in chitosan-coated calcium alginate microparticles. J Agric Food Chem. 2004; 52: 7300-7305.
Park JK, Chang HN. Microencapsulation of microbial cells. Biotechnol Adv. 2000; 18: 303-319.
Heller K. Probiotic bacteria in fermented foods: product characteristics and starter organisms. Am J Clin Nutr. 2001; 73: 374-379.
Gomes AMP, Malcata FX. Bifidobacterium. spp. and Lactobacillus acidophilus: biological, biochemical, technological and therapeutical properties relevant for use as probiotics. Food Sci Technol. 1999; 10: 139-157.
Lacroix C, Yildirim S. Fermentation technologies for the production of probiotics with high viability and functionality. Curr Opin Biotech. 2007; 18: 176-183.
Krasaekoopt W, Bhandari B, Deeth H. The influence of coating materials on some properties of alginate beads and survivability of microencapsulated probiotic bacteria. Int Dairy J. 2004; 14: 737-743.
Gbassi GK, Vandamme T, Ennahar S. Marchioni E. Microencapsulation of Lactobacillus plantarum spp in an alginate matrix coated with whey proteins. Int J Food Microbiol. 2009; 129: 103-105.
Ozer B, Kirmaci HA, Senel E, Atamer M, Hayaloglu A. Improving the viability of Bifidobacterium bifidum BB-12 and Lactobacillus acidophilus LA-5 in white-brined cheese by microencapsulation. Int Dairy J. 2009; 19: 22-29.
Mortazavian A, Razavi SH, Ehsani MR, Sohrabvandi S. Principles and methods of microencapsulation of probiotic microorganisms. Iran J Biotechnol. 2007; 5: 1-18.
Annan NT, Borza AD, Hansen LT. Encapsulation in alginate-coated gelatin microspheres improves survival of the probiotic Bifidobacterium adolescentis 15703T during exposure to simulated gastrointestinal condi-tions. Food Res Int. 2008; 41: 184-193.
Avonts L, Uytven EV, Vuyst LD. Cell growth and bacteriocin production of probiotic Lactobacillus strains in different media. Int Dairy J. 2004; 14: 947-955.
Kiviharju K, Leisola M, Eerikainen T. Optimization of a Bifidobacterium longum production process. J Biotechnol. 2005; 117: 299-308.
Chaplin MF, Kennedy JF. Carbohydrate analysis: A practical approach. Oxford University Press; IRL PRESS. 2nd Edition. UK. 1987.
Dubois M, Gillis KA, Hamilton JK, Rebers PA, Smith F. Colorimetric method for determination of sugars and related substance. Anal Chem. 1956; 28: 350-356.
Das A, Ray S, Raychaudhuri U, Chakraborty R. Microencapsulation of probiotic bacteria and its potential application in food technology. Int J Agric Environ Biotechnol. 2014; 7(1): 47-53.
Graff S, Hussain S, Chaumeil J, Charrueau C. Increased intestinal delivery of viable Saccharomyces boulardii by encapsulation in microspheres. Pharmaceut Res. 2008; 25: 1290-1296.
Trabelsi I, Bejar W, Ayadi D, Chouayekh H, Kammoun R, Bejar S, Salah R. Encapsulation in alginate and alginate coated-chitosan improved the survival of newly probiotic in oxgall and gastric juice. Int J Biol Macromol. 2013; 61: 36-42.
De Vries W, Stouthamer AH. Factors determining the degree of anaerobiosis of Bifidobacterium strains. Arch Microbiol. 1969; 65: 275-287.
Gonzalez R, Blancas A, Santillana R, Azaola A, Wacher C. Growth and final product formation by Bifido-bacterium infantis in aerated fermentations. J Appl Microbiol Biotechnol. 2004; 65: 606-610.
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