Lactic acid Production with in situ Extraction in Membrane Bioreactor
Applied Food Biotechnology,
Vol. 4 No. 1 (2017),
4 Dey 2017
,
Page 27-34
https://doi.org/10.22037/afb.v4i1.13686
Abstract
Background and Objective: Lactic acid is widely used in the food, chemical and pharmaceutical industries. The major problems associated with lactic acid production are substrate and end-product inhibition, and by-product formation. Membrane technology
represents one of the most effective processes for lactic acid production. The aim of this work is to increase cell density and lactic acid productivity due to reduced inhibition effect of substrate and product in membrane bioreactor.
Material and Methods: In this work, lactic acid was produced from lactose in membrane bioreactor. A laboratory scale membrane bioreactor was designed and fabricated. Five types of commercial membranes were tested at the same operating conditions (transmembrane pressure: 500 KPa and temperature: 25°C). The effects of initial lactose concentration and dilution rate on biomass growth, lactic acid production and substrate utilization were evaluated.
Results and Conclusion: The high lactose retention of 79% v v-1 and low lactic acid retention of 22% v v-1 were obtained with NF1 membrane; therefore, this membrane was selected for membrane bioreactor. The maximal productivity of 17.1 g l-1 h-1 was obtained
with the lactic acid concentration of 71.5 g l-1 at the dilution rate of 0.24 h−1. The maximum concentration of lactic acid was obtained at the dilution rate of 0.04 h−1. The inhibiting effect of lactic acid was not observed at high initial lactose concentration. The critical lactose concentration at which the cell growth severely hampered was 150 g l-1. This study proved that membrane bioreactor had great advantages such as elimination of substrate and product inhibition, high concentration of process substrate, high cell density,
and high lactic acid productivity.
Conflict of interest: There is no conflict of interest.
- ▪ Lactic acid ▪ Lactobacillus bulgaricus ▪ Lactose ▪ Nanofiltration ▪ Whey
How to Cite
References
Najafpour G, Hashemiyeh B, Asadi M, Ghasemi M. Biological treatment of dairy wastewater in an upflow anaerobic sludgefixed film bioreactor. Am Eurasian J Agric Environ Sci. 2008;4(2):251-257.
Panesar PS, Kennedy JF, Gandhi DN, Bunko K. Bioutilisation of whey for lactic acid production. Food Chem. 2007;105(1):1-14.
Tango M, Ghaly A. A continuous lactic acid production system using an immobilized packed bed of Lactobacillus helveticus. Appl Microbiol Biotechnol. 2002;58(6):712-720.
doi:10.1007/s00253-002-0970-3
Abdel-Rahman MA, Tashiro Y, Sonomoto K. Recent advances in lactic acid production by microbial fermentation processes. Biotechnol Adv. 2013;31(6):877-902.
Kadam SR, Patil SS, Bastawde KB, Khire JM, Gokhale DV.
Strain improvement of Lactobacillus delbrueckii NCIM 2365 for lactic acid production. Process Biochem. 2006;41(1):120-6.
Vodnar DC, Venus J, Schneider R, Socaciu C. Lactic acid
production by Lactobacillus paracasei 168 in discontinuous
fermentation using lucerne green juice as nutrient substitute. Chem Eng Technol. 2010;33(3):468-74.
Porro D, Bianchi MM, Brambilla L, Menghini R, Bolzani D,
Carrera V, et al. Replacement of a metabolic pathway for
large-scale production of lactic acid from engineered yeasts. Appl Environ Microbiol. 1999;65(9):4211-5.
Tango M, Ghaly A. Kinetic modeling of lactic acid production from batch submerged fermentation of cheese whey. Trans ASAE. 1999;42(6):1791-1780. doi: 10.13031/2013.13343.
Wee Y, Kim J, Ryu H. Biotechnological production of lactic acid and its recent applications. Food Technol Biotechnol. 2006;44(2):163-172.
Gätje G, Gottschalk G. Limitation of growth and lactic acid production in batch and continuous cultures of Lactobacillus helveticus. Appl Microbiol Biotechnol. 1991;34(4):446-449. doi: 10.1007/BF00180568.
Wang X, Wang Y, Zhang X, Feng H, Xu T. In-situ
combination of fermentation and electrodialysis with bipolar membranes for the production of lactic acid: Continuous operation. Bioresour Technol. 2013;(147):442-448.
Joglekar H, Rahman I, Babu S, Kulkarni B, Joshi A.
Comparative assessment of downstream processing options for lactic acid. Sep Purif Technol. 2006;52(1):1-17.
Evangelista RL, Nikolov ZL. Recovery and purification of
lactic acid from fermentation broth by adsorption.
Seventeenth symposium on biotechnology for fuels and
chemicals: Appl Biochem Biotechnol; 1996;471-480. doi:
1007/BF02941727.
Gyo Lee E, Moon S-H, Keun Chang Y, Yoo I-K, Nam Chang H. Lactic acid recovery using two-stage electrodialysis and its modelling. J Membr Sci. 1998; 145(1): 53-66.
Hulse JH. Biotechnologies: past history, present state and future prospects. Trends Food Sci Technol. 2004; 15(1): 3-18.
Pal P, Sikder J, Roy S, Giorno L. Process intensification in
lactic acid production: A review of membrane based
processes. Chemical Engineering and Processing: Process
Intensification. 2009;48:(11-12):1549-1559. doi:
1016/j.cep.2009.09.003.
Połom E, Szaniawska D. Rejection of lactic acid solutions by dynamically formed nanofiltration membranes using a statistical design method. Desalination. 2006;198(1):208-214. doi: 10.1016/j.d-esal.2006.04.002.
Moueddeb H, Sanchez J, Bardot C, Fick M. Membrane
bioreactor for lactic acid production. Journal of membrane
science. 1996;114(1):59-71.
Castillo Martinez FA, Balciunas EM, Salgado JM,
Domínguez González JM, Converti A, Pinheiro de Souza
Oliveira R. Lactic acid properties, applications and
production: A review. Trends Food Sci Technol. 2012;30(1):70-83.
Sikder J, Roy M, Dey P, Pal P. Techno-economic analysis of a membrane-integrated bioreactor system for production of lactic acid from sugarcane juice. Biochem Eng J. 2012;63:81-87.
Boontawan P, Kanchanathawee S, Boontawan A. Extractive fermentation of l-(+)-lactic acid by Pediococcus pentosaceus using electrodeionization (EDI) technique. Biochem Eng J. 2011;54(3):192-1999.
Diosady LL, Puzanov T. Membrane fermentation of lactic acid. International Journal of Applied Science and
Engineering. 2005;3(1):19-25.
Jeantet R, Maubois J, Boyaval P. Semicontinuous production of lactic acid in a bioreactor coupled with nanofiltration membranes. Enzyme Microb Technol. 1996;19(8):614-619.
Koyuncu I, Turan M, Topacik D, Ates A. Application of low pressure nanofiltration membranes for the recovery and reuse of dairy industry effluents. Water Sci Technol. 2000;41(1):213-221.
Li Y, Shahbazi A, Williams K, Wan C. Separate and
concentrate lactic acid using combination of nanofiltration
and reverse osmosis membranes. Biotechnology for Fuels and Chemicals: Appl Biochem Biotechnol.; 2008;147:1-9. doi:10.1007/s12010-007-8047-5.
Mulder M. Basic Principles of Membrane Technology Second Edition: Kluwer Academic Pub; 1996.
Büyükkileci AO, Harsa S. Batch production of L (+) lactic
acid from whey by Lactobacillus casei (NRRL B-441). J
Chem Technol Biotechnol. 2004;79(9):1036-40.
Cox G, MacBean R. Lactic acid production by Lactobacillus bulgaricus in supplemented whey ultrafiltrate [waste utilization]. Australian Journal of Dairy Technology.
;32(1):19-22.
Plessas S, Bosnea L, Psarianos C, Koutinas A, Marchant R, Banat I. Lactic acid production by mixed cultures of
Kluyveromyces marxianus, Lactobacillus delbrueckii ssp
bulgaricus and Lactobacillus helveticus. Bioresour Technol.
;99(13):5951-5955.
Schepers AW, Thibault J, Lacroix C. Continuous lactic acid production in whey permeate yeast extract medium with immobilized Lactobacillus helveticus in a two-stage process: Model and experiments. Enzyme Microb Technol.
;38(3):324-37.
Tejayadi S, Cheryan M. Lactic acid from cheese whey
permeate. Productivity and economics of a continuous
membrane bioreactor. Appl Microbiol Biotechnol.
;43(2):242-248. doi: 10.1007/B-F00172819.
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