Optimization of a Modified GS Medium for a Probiotic Strain (L. acidophilus ATCC4356)
Applied Food Biotechnology,
Vol. 1 No. 1 (2014),
25 September 2014
,
Page 25-29
https://doi.org/10.22037/afb.v1i1.7128
Abstract
Probiotics are defined as living microorganisms with beneficial effects on the host. Probiotics, according to the least negative effect on the body, are considered as a good alternative to chemical drugs. Lactobacillus acidophilus is used as a probiotic that is able to reduce cholesterol level in the blood. The effect of various concentrations of carbon, nitrogen and phosphorus for enhancing the biomass production of Lactobacillus acidophilus was examined. The response surface methodology based on Box–Wilson CCD was applied to explore the optimal medium composition. Glucose, yeast extract, K2HPO4 and KH2PO4 were selected as dependent variables. All experiments were run at 37°C for 31h under stationary conditions. By solving the regression equation and analyzing the response surface carton, optimal concentrations of the components were determined as glucose (5-8.75 g.l-1), yeast extract (36.75-39 g.l-1), K2HPO4 (0.1-0.2125 g.l-1) and KH2PO4 (0.3925-0.7075 g.l-1). Validation experiment confirmed that the optimized medium was comparable to the MRS medium (the most common medium for Lactobacillus acidophilus strain) in biomass production, having the advantages of economy andpracticality.- Probiotic
- Lactobacillus acidophilus
- Cholesterol removal
- GS culture medium
- Response surface methodology
How to Cite
References
Meei Y, Tseng W. Reduction of cholesterol by Lactobacillus acidophilus in culture Broth. J Food Drug Anal. 2000; 8: 97-102.
Al-Saleh AA, Metwalli, AAM, Abu-Tarboush HM. Bile salts and acid tolerance and cholesterol removal from media by some lactic acid bacteria and Bifidobacteria. J Saudi Soc Food Nutr. 2006; 1: 1-17.
He GQ, Kong Q, Ding LX. Response surface methodology for optimizing the fermentation medium of Clostridium butyricum. Lett Appl Microbiol; 2004; 39: 363-368.
Liong MT, Shah NP. Bile salt deconjugation ability, bile salt hydrolase activity and cholesterol co-precipitation ability of lactobacilli strains. Int Dairy J. 2005; 15: 391-398.
Ziarno M. In vitro cholesterol uptake by lactobacillus acidophilus isolates. Acta Sci Pol Technol Aliment. 2008; 7: 65-74.
Polak-berecka M, Wasko A, Kordowsk-wiater M, Podlesny M, Targonski Z, Kubik-Komar A. Optimization of medium composition for enhancing growth of Lactobacillus rhamnosus PEN using response surface methodology. Pol J Microbiol. 2010; 59: 113-118.
Sathyanarayanan J, Kunthala J, Gurumurthy K. Optimization of MRS media components using response surface methodology for the riboflavin production by Lactobacillus fermentum isolated from yoghurt sample. Int Food Res J. 2011; 18: 149-158.
Ataei SA. L(+) lactic acid production and separation from dairy waste (whey): In situ separation of lactic acid using ion exchange resins in automatic control of pH. J Kerman University Medical Sci. 2000; 7: 200-205.
Ataei SA, Farahani EV, Shojaosadat SA, Tehrani HA. Isolation of PHA-producing bacteria from a date syrup waste. Macromol Symp. 2008; 269: 11-16.
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