Analysis of the Molecular Docking of Piperine with Cyclooxygenase-2, Followed by an In-Vivo Study of Its Anti-Inflammatory and Analgesic Properties
International Pharmacy Acta,
Vol. 8 No. 1 (2025),
6 April 2025
,
Page e2: 1-8
https://doi.org/10.22037/ipa.v8i1.47213
Abstract
Piperine, the primary alkaloid in black pepper (Piper nigrum), is known for its promising biological activities. However, traditional extraction methods often face challenges such as low yield, high costs, and the risk of photodegradation. This study aimed to develop a simple, rapid, and efficient method for isolating piperine. Ethanol was used as the solvent for extraction, followed by isolation and purification through the Soxhlet method, which provided high-purity crystalline piperine, confirmed by physicochemical and spectroscopic analyses. In silico docking studies revealed that piperine forms a more stable complex with cyclooxygenase-2 (COX-2) than the native ligand (arachidonic acid), as evidenced by lower ΔGbind values. In vivo experiments demonstrated that piperine, administered at 50 mg/kg body weight, exhibited significant anti-inflammatory and analgesic effects within 60 to 180 minutes, with inhibition percentages comparable to aspirin at 150 mg/kg. This study successfully developed an efficient extraction method for piperine. It confirmed its significant biological activities, including enhanced COX-2 binding and practical anti-inflammatory and analgesic effects, highlighting its potential as a natural alternative to conventional treatments.
- Black pepper
- Piperine
- Extraction
- In silico
How to Cite
References
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