Evaluation of Antioxidant, Anti-Tyrosinase, and Anti-Melanoma Activities of Phlomis Rigida
International Pharmacy Acta,
Vol. 6 No. 1 (2023),
1 January 2023
,
Page e7: 1-6
https://doi.org/10.22037/ipa.v6i1.42686
Abstract
The aim of this study is to evaluate in vitro cytotoxicity of Phlomis rigida (P. rigida) aerial parts on the malignant melanoma cells as well as assess its antioxidant and anti-tyrosinase activities. The total phenolic and flavonoid contents along with in vitro antioxidant and anti-tyrosinase activities of P. rigida MeOH (80%) extract were determined using Folin-Ciocalteu, aluminum chloride, DPPH radical scavenging, and mushroom tyrosinase assays, respectively. The cytotoxicity of the extract was investigated by determination of the cell viability using MTT assay on the normal fibroblast (NIH3T3) and malignant melanoma (SKMEL-3) cells. The extract showed a weak scavenging activity with SC50 value higher than 5 mg/mL and the tyrosinase inhibitory activity with an IC50 value of 1.092 mg/mL. Interestingly, the extract was not toxic on NIH3T3 cells at all tested concentrations (0.0001-0.1 mg/mL), however, it could significantly reduce cell viability on SKMEL-3 cells, particularly at higher concentrations than 0.01 mg/mL (the IC50 ≈ 0.148 mg/mL). Based on our results, a selective cytotoxic effect against SKMEL-3 cells was found for the extract of P. rigida compared with NIH3T3 cells. Therefore, it is recommended as a good candidate for further investigation to discover bioactive natural agents in melanoma treatment
- Antioxidant
- Anti-tyrosinase
- Cell viability
- NIH3T3
- Phlomis rigida
- SKMEL-3
- 2023-11-14 (2)
- 2023-11-01 (1)
How to Cite
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