Sedative-Hypnotic Effects of Different Extracts and Fractions of Capparis Spinosa L. in Mice
International Pharmacy Acta,
Vol. 4 No. 1 (2021),
2 June 2021
Introduction: Capparis spinosa L. (Caper) is an aromatic plant growing in dry regions around the Mediterranean area. C. spinosa is shown to have several compounds such as tannins, sterols, alkaloids, polyphenols and flavonoids. Previous studies showed that flavonoids have sedative and hypnotic effects. The aim of this study was evaluating the sedative-hypnotic effects of various extracts and fractions of C. spinosa in different doses.
Methods and Results: Maceration method was used for extraction of the aerial part of the plant. All experiments conducted on male NMRI mice (18-25g weight). For evaluation the hypnotic and sedative effects of plant, open field and pentobarbital induced sleep test were used. In this study, animals were treated with different doses of aqueous extract, methanolic extract, methanolic fraction and dichloromethane fraction (i.p.). In pentobarbital induced sleep test, the duration of lack of righting reflex was reported. In open field test, the locomotor activities of mice were calculated by measuring total distance moved in the open field arena. According to the results, the dichloromethane fraction decreased the total distance moved in the open field test (ED50 = 66.6 mg/kg) and also increased the sleep duration in pentobarbital induced sleep test (ED50 =18.7 mg/kg) compared with other extract and fractions groups.
Conclusion: In conclusion, the dichloromethane fraction of C. spinosa has hypnotic and sedative effects compared to the other extract and fraction groups. Further studies are necessary to find the active components responsible for the several effects of the dichloromethane fraction and the exact mechanism of action of these effects.
- Capparis spinosa L.
- Sedative; Hypnotic
- Open field test
- Pentobarbital-induced sleep test
How to Cite
Moufid A, Farid O, M Eddouks. Pharmacological Properties of Capparis spinosa Linn. Int J Diabetol Vasc Dis Res. 2015 Jun 19;99–104.
Schmidt B, Ribnicky DM, Poulev A, Logendra S, Cefalu WT, Raskin I. A natural history of botanical therapeutics. Metabolism. 2008 Jul;57:S3–9.
Rishton GM. Natural Products as a Robust Source of New Drugs and Drug Leads: Past Successes and Present Day Issues. Am J Cardiol. 2008 May;101(10):S43–9.
Barnes PM, Bloom B, Nahin RL. Complementary and alternative medicine use among adults and children: United States, 2007. Natl Health Stat Report. 2008 Dec 10;(12):1–23.
Fici S. A taxonomic revision of the Capparis spinosa group (Capparaceae) from the Mediterranean to Central Asia. Phytotaxa. 2014 Jul 4;174(1):1.
Ahmadi M, Saeidi H. Genetic diversity and structure of Capparis spinosa L. in Iran as revealed by ISSR markers. Physiol Mol Biol Plants. 2018 May 10;24(3):483–91.
Mahla HR, Rathore VS, Singh D, Singh JP. Capparis decidua (Forsk.) Edgew.: an underutilized multipurpose shrub of hot arid region—distribution, diversity and utilization. Genet Resour Crop Evol. 2013 Jan 21;60(1):385–94.
Germanò MP, De Pasquale R, D’Angelo V, Catania S, Silvari V, Costa C. Evaluation of Extracts and Isolated Fraction from Capparis spinosa L. Buds as an Antioxidant Source. J Agric Food Chem. 2002 Feb;50(5):1168–71.
Aytaç Z, Kinaci G, Ceylan A. Yield and some morphological characteristics of caper (Capparis spinosa L.) population cultivated at various slopes in aegean ecological conditions. Pakistan J Bot. 2009;41(2):591–6.
Tlili N, Elfalleh W, Saadaoui E, Khaldi A, Triki S, Nasri N. The caper (Capparis L.): Ethnopharmacology, phytochemical and pharmacological properties. Fitoterapia. 2011 Mar;82(2):93–101.
Çaliş İ, Kuruüzüm A, Rüedi P. 1H-Indole-3 acetonitrile glycosides from Capparis spinosa fruits. Phytochemistry. 1999 Apr;50(7):1205–8.
Tlili N, Khaldi A, Triki S, Munné-Bosch S. Phenolic Compounds and Vitamin Antioxidants of Caper (Capparis spinosa). Plant Foods Hum Nutr. 2010 Sep 29;65(3):260–5.
Moutia M, El Azhary K, Elouaddari A, Al Jahid A, Jamal Eddine J, Seghrouchni F, et al. Capparis spinosa L. promotes anti-inflammatory response in vitro through the control of cytokine gene expression in human peripheral blood mononuclear cells. BMC Immunol. 2016 Dec 2;17(1):26.
Zhang H, Ma Z. Phytochemical and Pharmacological Properties of Capparis spinosa as a Medicinal Plant. Nutrients. 2018 Jan 24;10(2):116.
Gillani Q, Iqbal S, Arfa F, Khakwani S, Akbar A, Ullah A, et al. Effect of GABAB Receptor Antagonist (CGP35348) on Learning and Memory in Albino Mice. Sci World J. 2014;2014:1–6.
Zhang J, Gao J, Guo G, Li S, Zhan G, Xie Z, et al. Anesthesia and surgery induce delirium-like behavior in susceptible mice: the role of oxidative stress. Am J Transl Res. 2018;10(8):2435–44.
Jahani R, Mojab F, Mahboubi A, Nasiri A, Tahamtani A, Faizi M. An In-Vivo Study on Anticonvulsant, Anxiolytic, and Sedative-Hypnotic Effects of the Polyphenol-Rich Thymus Kotschyanus Extract; Evidence for the Involvement of GABAA Receptors. IJPR. 2019;18(3):1456–65.
Tabatabai SA, Rezaee Zavareh E, Reyhanfard H, Alinezhad B, Shafaghi B, Sheikhha M, et al. Evaluation of Anxiolytic, Sedative-hypnotic and Amnesic Effects of Novel 2-phenoxy phenyl-1,3,4-oxadizole Derivatives Using Experimental Models. IJPR. 2015;14(Suppl):51–7.
Suleyman H, Guvenalp Z, Kizilkaya M, Demirezer Lo. Sedative Effect of Centranthus longiflorus ssp. longiflorus in Rats and the Influence of Adrenalectomy on its Effect. Yakugaku Zasshi. 2007 Aug 1;127(8):1263–5.
Hajiaghaee R, Faizi M, Shahmohammadi Z, Abdollahnejad F, Naghdibadi H, Najafi F, et al. Hydroalcoholic extract of Myrtus communis can alter anxiety and sleep parameters: a behavioural and EEG sleep pattern study in mice and rats. Pharm Biol. 2016 Oct 2;54(10):2141–8.
Korkmaz A, Kolankaya D. Protective Effect of Rutin on the Ischemia/Reperfusion Induced Damage in Rat Kidney. J Surg Res. 2010 Dec;164(2):309–15.
Pérez-Ortega G, Guevara-Fefer P, Chávez M, Herrera J, Martínez A, Martínez AL, et al. Sedative and anxiolytic efficacy of Tilia americana var. mexicana inflorescences used traditionally by communities of State of Michoacan, Mexico. J Ethnopharmacol. 2008 Mar;116(3):461–8.
S PB, R SS, Upendra K, G KP. Current Trends in Herbal Medicines. J Pharm Res. 2010;3(1):109–13.
Mahboubi M, Mahboubi A. Antimicrobial activity of Capparis spinosa as its usages in traditional medicine. Herba Pol. 2014;60(1):39–48.
Miraldi E, Ferri S, Mostaghimi V. Botanical drugs and preparations in the traditional medicine of West Azerbaijan (Iran). J Ethnopharmacol. 2001 May;75(2–3):77–87.
Ali-Shtayeh M., Yaghmour RM-R, Faidi Y., Salem K, Al-Nuri M. Antimicrobial activity of 20 plants used in folkloric medicine in the Palestinian area. J Ethnopharmacol. 1998 Apr;60(3):265–71.
Darwish RM, Aburjai T, Al-Khalil S, Mahafzah A. Screening of antibiotic resistant inhibitors from local plant materials against two different strains of Staphylococcus aureus. J Ethnopharmacol. 2002 Mar;79(3):359–64.
Tesoriere L, Butera D, Gentile C, Livrea MA. Bioactive Components of Caper (Capparis spinosa L.) from Sicily and Antioxidant Effects in a Red Meat Simulated Gastric Digestion. J Agric Food Chem. 2007 Oct;55(21):8465–71.
Mishra S, Tomar P, Lakra N. Medicinal and food value of Capparis—a harsh terrain plant. Indian J Tradit Knowl. 2007;06(1):230–8.
Matthäus B, Özcan M. Glucosinolates and Fatty Acid, Sterol, and Tocopherol Composition of Seed Oils from Capparis spinosa Var. spinosa and Capparis ovata Desf. Var. canescens (Coss.) Heywood. J Agric Food Chem. 2005 Sep;53(18):7136–41.
Khanavi M, Ara L, Khavassi N, Hajimehdipoor H. Capparis spinosa: a comparative study of raw and processed fruits. J Med Plants. 2020 Mar 1;1(73):91–9.
Inocencio C, Rivera D, Alcaraz F, Tomás-Barberán FA. Flavonoid content of commercial capers ( Capparis spinosa, C. sicula and C. orientalis ) produced in mediterranean countries. Eur Food Res Technol. 2000 Dec 5;212(1):70–4.
Fu XP, Wu T, Abdurahim M, Su Z, Hou XL, Aisa HA, et al. New spermidine alkaloids from Capparis spinosa roots. Phytochem Lett. 2008;1(1):59–62.
- Abstract Viewed: 116 times
- PDF Downloaded: 52 times