Use of Green Fluorescent Protein (GFP) Vector in Classical Restriction Enzyme-based Cloning Methods of Gateway Cloning System
International Pharmacy Acta,
Vol. 2 No. 1 (2019),
16 December 2019
,
Page 2e6:1-5
https://doi.org/10.22037/ipa.v2i1.22719
Abstract
Generating of an expression clone that can produce the pharmaceutical proteins in an efficient and soluble form at high levels is considered as an important step in pharmaceutical industry. Recombination-based cloning could be a quick and efficient way for generating expression vectors. Thus, both efficient and robust subcloning is vital for the construction of gene expression vectors. In this study, we used the traditional restriction enzyme-based cloning methods for generation of expression-ready clones by the most well-known commercial cloning technologies, Gateway.
- GFP
- Gateway cloning technology
- pEGFP-N1
- pTracer-SV40
- plasmid
- PCR
How to Cite
References
References
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